Alignments for a candidate for pccB in Oleispira antarctica

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccB in Oleispira antarctica

Align acyl CoA carboxylase carboxyltransferase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate WP_046008372.1 OLEAN_RS05310 acetyl-CoA carboxylase

Query= metacyc::MONOMER-13598
         (524 letters)



>NCBI__GCF_000967895.1:WP_046008372.1
          Length = 555

 Score =  309 bits (792), Expect = 2e-88
 Identities = 185/497 (37%), Positives = 276/497 (55%), Gaps = 32/497 (6%)

Query: 26  QGGGEDKIKAQHDKGKLTARERLALLFDEGSFNEIMPLATTRATEFGLDKNKFYGDGVVT 85
           +  G + I  QH K ++T  ER+++L DE       P    +     LD     G  +VT
Sbjct: 55  KAAGINAITRQHAKKRMTIWERISVLTDES------PTVLFQNWGKNLD-----GASLVT 103

Query: 86  GWGKIEGRTVFAFSQDFTELGGTLGETHANKIGKVYELALKVGAPVIGINDSGGARIQEG 145
              KI GR V  +  DFT   G++  T+  K+ +++ELA K   P++G+NDS GA +  G
Sbjct: 104 AIVKINGRDVALYGHDFTVRAGSMDATNGKKLARLFELAGKRKIPLVGLNDSAGAYVPAG 163

Query: 146 AIALEGYATVFKMNTLASGVIPQITIMAGPAAGGAVYSPALTDFIIMIKGDAYYMFVTGP 205
              L+GYA  F      SGV+P I  M G  AGG  Y P    F  MI+ +  +  +TGP
Sbjct: 164 VGGLDGYAEAFTALRKISGVVPSIMCMFGFNAGGGSYLPRQGSF--MIQPNNTFFGLTGP 221

Query: 206 EITKVVLGEEVTFQDLGGAVVHATKSGVVHFLAENEQDAISIAKRLLSYLPSNNMEDPPY 265
            + K VLGE+VT  +LGG  VH+ +SGV  F+ ++E  A+   KRLL+Y+P+NN E  PY
Sbjct: 222 GVVKSVLGEDVTPDELGGPSVHS-QSGVTDFVVKDEVSALKKVKRLLNYIPNNNSEFAPY 280

Query: 266 MDTGDPSDRETKDVESIV------PTDSAKPFDMREVIYRIVDNGEFMEVHRHWAQNIVV 319
            +T DP DR T D++ ++      P+    P D+  +I ++ D+G+FME+    A+N + 
Sbjct: 281 QETSDPVDRRTWDIDVLLKKAFNSPSGFNTPMDVTIMIQQLCDHGDFMEIQPDRARNTIT 340

Query: 320 GFARVAGNVIGIVANNSNTLGAAIDIDAADKAARFIRFCDAFNIPLLSLVDTPGYIPGTE 379
              R+ GNVIG VANNS      IDI AA K ARFIRFC+ +NIP++ + DT G++PG +
Sbjct: 341 ALGRMGGNVIGFVANNSAVSSGQIDIAAAYKNARFIRFCNLYNIPVIFMEDTTGFLPGKD 400

Query: 380 QEYKGIIRHGAKMLYAFSEATVPKISVIIRKSYGGAHIAMSIKNLGADLVYAWPTAEIAV 439
           QE +GI++ G  ML A  +   P+  +IIR ++GGA+ + +    GAD V A P+   AV
Sbjct: 401 QEAQGIVQAGRAMLDAIIDLRTPRFLLIIRNAFGGAYASYNNYPTGADFVIALPSTRAAV 460

Query: 440 TGPEGAVRILYKRD-------IQNSQNPDEFLKQKIA----EYKKLFANPYWAAEKGLID 488
            GP G V  +YK +       ++N Q   ++ K++ A     Y++   NP  A   G I 
Sbjct: 461 MGPAG-VEFVYKNELRAIRSAVKNKQQTAQWAKEQEALLAKRYEEELMNPNEALSLGSIS 519

Query: 489 DVIEPKDTRRIIVNALS 505
            ++ P D R+++   +S
Sbjct: 520 QIVMPSDLRKVLSENMS 536


Lambda     K      H
   0.318    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 715
Number of extensions: 34
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 524
Length of database: 555
Length adjustment: 35
Effective length of query: 489
Effective length of database: 520
Effective search space:   254280
Effective search space used:   254280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources