Align 3-hydroxybenzoate--CoA/4-hydroxybenzoate--CoA ligase; 3-hydroxybenzoyl-CoA synthetase; EC 6.2.1.27; EC 6.2.1.37 (characterized)
to candidate WP_051302719.1 A3GO_RS24835 benzoate-CoA ligase family protein
Query= SwissProt::Q9AJS8 (523 letters) >NCBI__GCF_000428045.1:WP_051302719.1 Length = 536 Score = 522 bits (1344), Expect = e-152 Identities = 253/519 (48%), Positives = 357/519 (68%), Gaps = 4/519 (0%) Query: 3 EQLQPQQSMNAADEIIGRPLAQGLGEQTAMLCAERSITYRELDAATNRHGNALRAHGVGK 62 E+ P +MNA +E+IG L++GL +QTA++ +S+TY ELD NR+ +ALR GV + Sbjct: 6 ERYFPSGAMNAVEELIGPALSKGLEQQTALIGDTKSLTYGELDLLVNRYSDALRTAGVVR 65 Query: 63 GDRVLFLMDDSPELVAAYLGTLRIGAVAVALNVRLAPRDVLYVIQDSACRLLYIDAEFLH 122 +RVLF+++DSP+ VAAYLG + +GAV++A NVR A +D+L+VI+DS C +L IDA +L Sbjct: 66 ENRVLFMLNDSPDFVAAYLGAMHMGAVSIAFNVRSAAKDLLFVIEDSNCTMLVIDAAYLP 125 Query: 123 LYQQIAGELEQPPQVVVRGD-EAPAPAIIAFKHFLDGQAATLESVQVAPDDVAYWLYSSG 181 +Y QIA ++ P +VVV GD E P + +F ++ A +++V+PDD+A+W+Y+SG Sbjct: 126 IYLQIADRIKHPLKVVVDGDYEGDLPTLGSF---VESGATRSPALKVSPDDMAFWVYTSG 182 Query: 182 TTGRPKAVMHAHRSVLIADRLEREYFGIKPGDRVFTTSKMFFGWSLGHSLMGGLQCGATV 241 TTG PKA +H H V+IAD RE G++PGDR+F+TSK+FF ++LGHSL+GGL+ AT+ Sbjct: 183 TTGTPKAAVHLHHDVIIADLHMRENLGVEPGDRIFSTSKLFFAYALGHSLLGGLRSCATI 242 Query: 242 IVAPGWPDAERVMATAARHRPTILFSTPVMYRNLLREGAGESAAMRDIRHFVSAGEKLPE 301 I+ WPDA + RH+P ++FS P +YRNLLR+G ++A + +R +VSAGEKLP Sbjct: 243 ILTKQWPDAAAIAELIRRHQPDLVFSVPTLYRNLLRDGVADTAPFKSVRVYVSAGEKLPV 302 Query: 302 NIGQQWLDTFGIPITEGIGASETVFLFLCARPDAYRIGSCGKRVPWAEVRLLDELGNEIT 361 + QW G PI EGIG SET+FL++ +R GSCGK VPWAE++L+DE I+ Sbjct: 303 KLFDQWRLATGRPILEGIGTSETLFLYVTNSIKQFRSGSCGKPVPWAEIKLMDESEQAIS 362 Query: 362 TPDTPGLIAIRMASQFVGYWKLPETTEKALRDGWYYPGDMFSFDADGFWYHNGRADDMLK 421 T +TPG + +RM S YW + + K+ WY GDMF D DG+WYH GR D+MLK Sbjct: 363 TTETPGQLWVRMPSVADRYWNRQDQSRKSFHGHWYKTGDMFRVDEDGWWYHLGRGDEMLK 422 Query: 422 ISGQWVSPGEIESCASAVPGIAEAVVVAVPNDDGLTRLTLFIVPEDPSASQQKLSEAWMT 481 ISGQWVSP EIE+CA VPG+ A V+ N+D L R LF+VP+ L++ Sbjct: 423 ISGQWVSPAEIEACALTVPGVEAAAVIGYSNEDSLVRSALFVVPQQRGTDDDMLADKIRQ 482 Query: 482 TLRGTLSIYKCPRTIQFLEELPRTATGKVQKYRLRDMLQ 520 L TLSIYKCPR I+ ++E+P TA+GK+QKY+LR++L+ Sbjct: 483 ALIQTLSIYKCPRIIRLVDEMPCTASGKMQKYKLRELLR 521 Lambda K H 0.321 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 822 Number of extensions: 43 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 523 Length of database: 536 Length adjustment: 35 Effective length of query: 488 Effective length of database: 501 Effective search space: 244488 Effective search space used: 244488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory