Alignments for a candidate for treEIIA in Desulfobulbus mediterraneus DSM 13871

GapMind for catabolism of small carbon sources

Alignments for a candidate for treEIIA in Desulfobulbus mediterraneus DSM 13871

Align D-trehalose PTS system, I, HPr, and IIA components (characterized)
to candidate WP_051305559.1 G494_RS22685 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::WCS417:GFF4500
         (838 letters)



>NCBI__GCF_000429965.1:WP_051305559.1
          Length = 600

 Score =  305 bits (782), Expect = 4e-87
 Identities = 207/597 (34%), Positives = 316/597 (52%), Gaps = 48/597 (8%)

Query: 268 ATLLRGVCASPGSAFGQVVQVT-----------DPELVITEQGTGGATERAALTRGLLAA 316
           +TLL+G+  S G   G+VV +             PE +          E A   + +  A
Sbjct: 15  STLLQGIGVSAGIVVGRVVVIKRQTCRAGWYQLPPEFI--------QEEVARFQQAVTTA 66

Query: 317 NEALQVLQDKAAGSA--QAEIFRAHQELLEDPTLLEHAHRLLG-EGKSAAFAWNSATLAT 373
            + L+ L++  A        I  +H  +L+D  + +    ++  +  +A +A   A    
Sbjct: 67  EQELRQLRENLADDLADSLSIIDSHILMLKDRMIYDQTVEIIRLKNVNAEWALAQALTKV 126

Query: 374 VTLFQGLGNALIAERAADLADVGQRVLKLILGIQDSAWDL-PERAILIAEQLTPSQTASL 432
              F  + +  I ER AD+  V  RV  ++ G +        E  +++A   +P  T  L
Sbjct: 127 KQRFDTIADPYIKERYADVKHVADRVFGILFGREPYQLVTGQEPVVVVANDFSPEDTMQL 186

Query: 433 DTRKVLGFVTVAGGATSHVAILARALGLPAICGVPAQVLALANGKQVLLDADKGELHLEP 492
            T  +LG +   GG TSH +I+AR+L +PA+ G+     ++A G  V++D D G + + P
Sbjct: 187 HTENILGLLAERGGMTSHTSIVARSLNIPAVIGLTHITGSIATGDLVIIDGDAGTVIVAP 246

Query: 493 NLAEIEQLEAARKH--QVLRHQRDVAQ-ASLPATTRDGHHVEVTANVASLQEVEHALTLG 549
           +    EQ++A R+   ++     ++AQ   L ATT DG+ V+++ANV   +E+   L  G
Sbjct: 247 SP---EQIKAYRRQALELNAGAGELAQYIPLSATTFDGYQVQLSANVEMTEELASVLRFG 303

Query: 550 GEGVGLLRSEFLYLDRNRAPSPEEQAGTYTAIARALGT--ERNLVVRTLDVGGDKPLAYV 607
            +G+GL RSEF +  ++  P   ++A  YT   + L T     + VRTLDVGGDK L ++
Sbjct: 304 SQGIGLFRSEFDFFHQSCHP---DEAALYTTYRQLLETLAPHPVTVRTLDVGGDKFLDHL 360

Query: 608 P-----MDAETNPFLGLRGIRLCLERPQLLREQFRAILASAGFARLHIMLPMVSLLSELH 662
           P     +D E NP LGLR IR  L   +L   Q RA+L ++   RL I+ P+VS L+ELH
Sbjct: 361 PGHLSQLDPERNPALGLRSIRFSLYESELFSMQLRALLRASVHGRLRILFPLVSSLAELH 420

Query: 663 LARKILEE-----EALALGLTELPKLGIMIEVPSAALMADVFAPHVDFFSIGTNDLTQYT 717
             + IL       E   L      +LG+M+EVPSA ++ADVFA  VDF ++GTNDL QY+
Sbjct: 421 AVKAILRTIIGQLEEDGLDYEPQVELGVMVEVPSAVILADVFAKEVDFLAVGTNDLIQYS 480

Query: 718 LAMDRDHPRLANQADSFHPAVLRLIATTVKAAHAHGKWVGVCGALASEALAVPVLIGLGV 777
           LA+DR +  +A+  D FHPAVLR+I  T+ A HA G  V VCG +A + L   VL GLGV
Sbjct: 481 LAIDRSNQHVAHMYDPFHPAVLRMIKQTIDAGHAQGIPVSVCGEMAGDILCATVLFGLGV 540

Query: 778 DELSVSVPLIPTIKATVRELDLADCQIIARQVLGLEEAAEVREA----LRQYHAATV 830
           DELS+   +IP IK  +R L   + Q++ +++L   +  +V+      LRQY+   V
Sbjct: 541 DELSMRPAVIPYIKRLLRHLRYDELQLLGQEILHCVDGRQVQNQLMAFLRQYYPEKV 597


Lambda     K      H
   0.318    0.133    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 996
Number of extensions: 52
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 838
Length of database: 600
Length adjustment: 39
Effective length of query: 799
Effective length of database: 561
Effective search space:   448239
Effective search space used:   448239
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources