Align Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (characterized)
to candidate WP_057507601.1 ABB28_RS05090 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha
Query= reanno::Dino:3607308 (681 letters) >NCBI__GCF_001431535.1:WP_057507601.1 Length = 660 Score = 511 bits (1317), Expect = e-149 Identities = 306/691 (44%), Positives = 394/691 (57%), Gaps = 43/691 (6%) Query: 1 MFKKILIANRGEIACRVIKTARKMGIQTVAIYSDADRNALHVRMADEAVHIGPSPANQSY 60 MF KILIANRGEIACRVI T R++GI TVA+YSDADR A HVR+ADEA+ IGP+PA +SY Sbjct: 1 MFTKILIANRGEIACRVIATCRRLGIATVAVYSDADRTARHVRLADEAIAIGPAPAAESY 60 Query: 61 IVIDKVMDAIRQTGAEAVHPGYGFLSENKLFAEALEKEGVAFIGPPANAIEAMGDKITSK 120 + D +++A R++GA+A+HPGYGFLSEN FAEA GV FIGPPA+AI AMGDK +K Sbjct: 61 LRADVILEAARRSGAQAIHPGYGFLSENAAFAEACAAAGVVFIGPPASAIRAMGDKSAAK 120 Query: 121 KIAQEANVSTVPGYMGLIADADEAVKISGEIGYPVMIKASAGGGGKGMRIAWNDAEAREG 180 + Q+A V PGY G D + IGYPV+IKASAGGGGKGMR + A E Sbjct: 121 ALMQQAGVPLTPGYHGDDQDPQFLRGQADAIGYPVLIKASAGGGGKGMRRVDDSAAFIEA 180 Query: 181 FQSSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADTHGNCIYLGERECSIQRRNQKVVE 240 S + EA ++FG+D + +EK+V +PRHIEIQV D HGN ++L ER+CS+QRR+QKV+E Sbjct: 181 LASCQREARSAFGNDHVLVEKYVLRPRHIEIQVFGDGHGNVVHLFERDCSVQRRHQKVLE 240 Query: 241 EAPSPFLDEATRKAMGEQSCALAQAVGYASAGTVEFIVDGDRNFYFLEMNTRLQVEHPVT 300 EAP+P + R AMG+ + A+AVGY AGTVEFI D +FYF+EMNTRLQVEHPVT Sbjct: 241 EAPAPGMTAERRAAMGKAAVDAARAVGYVGAGTVEFIASPDGDFYFMEMNTRLQVEHPVT 300 Query: 301 ELITGVDLVEQMIRVAAGEKLPMTQDDVTLTGWAIENRLYAEDPYRNFLPSIGRLTRYRP 360 E ITG DLV +RVAAG+ LP QD++ + G A+E RLYAED + FLPSIG L R Sbjct: 301 EFITGTDLVAWQLRVAAGQPLPKRQDELAIGGHALEARLYAEDADKGFLPSIGTLRHLRL 360 Query: 361 PVEVAAGPLEANGKWHGDAPSGPTAVRNDTGVYEGGEISMYYDPMIAKLCTWGPDRPAAI 420 P E A VR DTGV +G I+ +YDPMIAKL W DR AA+ Sbjct: 361 PPESA-------------------HVRVDTGVEQGDTITPFYDPMIAKLIVWDVDRDAAL 401 Query: 421 EAMRNALDGFEVEGIGHNLPFVAAVMDHPVFIKGEMTTAFIKEQYPDGFEGVTLGAADLT 480 M+ AL +V G+ N F+ ++ F + ++ TA I E+ D G A L Sbjct: 402 RRMQQALADCQVVGVTTNAGFLRRLVGTDSFAQAKLDTALI-EREQDALAAPAAGEAALW 460 Query: 481 RLAAAAAAMFRVAEIRRTRISGTLDNHERMVGTDWVVTAQDARF----------DVTIDA 530 LAA A V R + W + A +R + + Sbjct: 461 TLAAIGA----VVTTARAAVDARDPRSPWQAQDGWRIGASASRLLPLQQGERQHSLRVQT 516 Query: 531 DPGGSTVRFADGTAHRVTSRWTPGDSLATVEIDGAPMVLKVDKITSGFRMRFRGADVKVH 590 PGG V+ A +V+ R+ G V +DG + V + + + Sbjct: 517 QPGGWQVQRDAEPAQQVSGRFADGQ--LDVALDGQRWRVGVQRDDGQVYLFTAQGQQRFS 574 Query: 591 VRTPRQAELNDLMPEKLPPDTSKMLLCPMPGLVVKIDVEVGDEVQEGQALCTVEAMKMEN 650 V P E+ D LL PMPG +V VE G +V G L +EAMKME+ Sbjct: 575 VHDPVGES------EQATADAGS-LLAPMPGRIVATLVEPGSKVSRGTPLVVLEAMKMEH 627 Query: 651 ILRAEKTATVTKINAGAGDSLAVDDVIMEFE 681 L A TV A AG+ + V+++FE Sbjct: 628 TLAAPADGTVHGYRAKAGEQVGDGAVLVDFE 658 Lambda K H 0.318 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1188 Number of extensions: 60 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 681 Length of database: 660 Length adjustment: 39 Effective length of query: 642 Effective length of database: 621 Effective search space: 398682 Effective search space used: 398682 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory