Alignments for a candidate for sglS in Stenotrophomonas chelatiphaga DSM 21508

GapMind for catabolism of small carbon sources

Alignments for a candidate for sglS in Stenotrophomonas chelatiphaga DSM 21508

Align SSS sodium solute transporter (characterized, see rationale)
to candidate WP_057509052.1 ABB28_RS13160 sodium transporter

Query= uniprot:L0FZF3
         (547 letters)



>NCBI__GCF_001431535.1:WP_057509052.1
          Length = 520

 Score =  544 bits (1402), Expect = e-159
 Identities = 282/549 (51%), Positives = 375/549 (68%), Gaps = 31/549 (5%)

Query: 1   MTFNTLDLVVFVAYCLLIITMGIVVSREKKGHVKDSKDYFLASKALPWWAVGASLIASNI 60
           M  + LD  + + Y   +  +   VSREK GH KD+KDYFLASK+LPWWAVGASLIA+NI
Sbjct: 1   MQLSVLDTSIVLVYLAGVFVLAQWVSREKAGHAKDAKDYFLASKSLPWWAVGASLIAANI 60

Query: 61  SAEQFIGMSGSGFALGLAISTYEWMAAATLLVVAIFFLPIYLKEGIYTMPQFLNRRYDGR 120
           SAEQ IGMSGSG+A+GLAI++YEWMAA TLL+V  +FLP++L+ GIYTMPQFL +RY  R
Sbjct: 61  SAEQIIGMSGSGYAIGLAIASYEWMAALTLLIVGKWFLPVFLRNGIYTMPQFLEQRYGTR 120

Query: 121 VRTVMAIFWLLIYVFVNLTSVLYLGALSLETIMGVPLTYGIIGLALFAMVYSIYGGLKAV 180
           +RT+MA+FWL +YVFVN+TS+L+LGA+++  + G+     ++ + +FA++Y +YGGLKAV
Sbjct: 121 IRTLMAVFWLGLYVFVNVTSILWLGAIAVSQVTGMDQMLAVVLIGVFALLYQLYGGLKAV 180

Query: 181 AWTDVVQVVFLVAGGLATTYLALSLVGDG-DVWEGIGILRKAAPSHFSMIIEKGEMMIPD 239
           A TD+VQV  LV GGL  T L LS +G+G  +  G+  L +A P HF MI+ K  +    
Sbjct: 181 ALTDIVQVSLLVLGGLLVTGLTLSQLGEGAGIVAGMQRLWEAHPGHFEMILSKDNLF--- 237

Query: 240 GSGGSRDAYLDLPGLSVLIGGMWIVNLNYWGCNQYITQRALAAKSLGEAQTGMVFAGFLK 299
                   Y DLPG+SVL+GG+WI+NL+YWG NQYI QRALAAK +GEAQ G++FA FLK
Sbjct: 238 --------YKDLPGISVLVGGLWIMNLSYWGFNQYIIQRALAAKDIGEAQKGVLFAAFLK 289

Query: 300 LLMPLIVVIPGIAAYVIVQKGADASFIESMTDPVTGLAKSDRAYPTLLHLLPPGLKGLAF 359
           LLMPLIVV+PGIAA ++                  GL   D+AYPT++ LLP G+ G+ F
Sbjct: 290 LLMPLIVVVPGIAAVML----------------APGLDAPDQAYPTMMRLLPTGILGMVF 333

Query: 360 AALTAAIVSSLASMANSTSTIFTIDIYKEFFNKNVSEGKQVTIGRITAVVAFIIAAIVA- 418
           AAL AAIV+SLAS  NS +TIFT+D Y +    +  E   V +GRI A V  ++  + A 
Sbjct: 334 AALVAAIVASLASKINSVATIFTLDFYAQRKGPH-DEATLVRVGRIAAAVTVVLGILAAK 392

Query: 419 PQLRQLDQAFQYIQEYTGFVSPGVFAIFIFGFFWKKTTSNAALTAAVLTIPLSAAFKVIT 478
           P L   DQAFQYIQEYTGF +PG+  IF+ G FWK+     AL AAV +  LS   K+  
Sbjct: 393 PLLGSFDQAFQYIQEYTGFFTPGIVVIFLLGLFWKRANEAGALAAAVGSFVLSVVLKLAW 452

Query: 479 PNLPFIDRMGVVFLVLSVLIIAISLYEGKGKDSKKAIEVDAELFSTSTKFKVGAVLICGI 538
           P+LPFIDR+G+VFL+  +L + +SL       +K  +  D  +++T   F VGA+ +  I
Sbjct: 453 PSLPFIDRVGLVFLLALLLAVGVSL-ATPAAPAKDVVRTDDVVYATRPSFNVGAIAVVAI 511

Query: 539 LVALYSVFW 547
           L+ALY++FW
Sbjct: 512 LIALYTLFW 520


Lambda     K      H
   0.326    0.140    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 853
Number of extensions: 31
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 547
Length of database: 520
Length adjustment: 35
Effective length of query: 512
Effective length of database: 485
Effective search space:   248320
Effective search space used:   248320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources