Alignments for a candidate for betS in Stenotrophomonas chelatiphaga DSM 21508

GapMind for catabolism of small carbon sources

Alignments for a candidate for betS in Stenotrophomonas chelatiphaga DSM 21508

Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate WP_057687578.1 ABB28_RS17180 high-affinity choline transporter BetT

Query= SwissProt::G3XCN6
         (706 letters)



>NCBI__GCF_001431535.1:WP_057687578.1
          Length = 659

 Score =  511 bits (1317), Expect = e-149
 Identities = 270/647 (41%), Positives = 395/647 (61%), Gaps = 18/647 (2%)

Query: 56  PVF-VGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFSMLFLAF 114
           PVF V SV V+A  V + +  P  A  +    Q    S  GW YLL++ ++L  ++  A 
Sbjct: 16  PVFAVASVIVVAFAVFVSLF-PLGAGRMLMQAQDWASSNVGWYYLLAMTLYLVFVVVAAL 74

Query: 115 SRYGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASPPEAEPLT-IA 173
           S YG +KLG D  EPEF YLSW  MLFAAG+ I L +F V EP+THF  PP+ +P    A
Sbjct: 75  SPYGSIKLGADHDEPEFSYLSWAGMLFAAGISITLFFFCVSEPLTHFMQPPQGDPAAGEA 134

Query: 174 AQREAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKEGIHGPIGHV 233
             R+AM + F HWG+H W ++++  +++AYF YR+NLPL +RS LYPL+   I+GPIG+ 
Sbjct: 135 GARQAMQLLFLHWGLHGWGVFALAAMAMAYFAYRHNLPLALRSALYPLIGNRINGPIGYT 194

Query: 234 VDIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAIATISVVTGV 293
           VD   I  T+FGL   +GFG+L +N+GL +L  +P +  VQ+ L+ ++   A    VTGV
Sbjct: 195 VDALGIVATVFGLGADMGFGVLHLNAGLAHLFDVPHTHGVQITLIVLMMGAAVAVAVTGV 254

Query: 294 EKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIYAYEPRP 353
           EKGVR +S  N+ LA+ L+LF+L  GPT  L+   +QN G YL S+V ++F++YAY  +P
Sbjct: 255 EKGVRWMSNINMLLAIALVLFMLFAGPTQYLLGTLMQNAGDYLGSVVGKSFDVYAYGGKP 314

Query: 354 -WIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFLWMTVFGNTA 412
            W+ SWT+FYWAWWI WSPFVG+FIARISRGRT+R+FV  VL +P  FT  W+++FGN+A
Sbjct: 315 GWMGSWTVFYWAWWIGWSPFVGLFIARISRGRTIRQFVFGVLLIPLGFTLAWLSIFGNSA 374

Query: 413 IYVDTTIANGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVTSSDSGSLVI 472
           +         +LA+    D    L+   E  PW      + VL+  +FFVTS+DSG++V+
Sbjct: 375 LDQVLHHGQSQLAQLAIDDPPTVLYALLESYPWSRAVIAVTVLVSFVFFVTSADSGAVVL 434

Query: 473 DTIASGG---ETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTALPFSLVMLIL 529
            T++S G   E   P   R+FW +++ +V A LL  G + AL+SA +  +LPFS V+L++
Sbjct: 435 STLSSHGGAPEDDGPRWLRVFWGTVTAVVTAGLLLAGNMDALKSAVVLASLPFSAVLLLM 494

Query: 530 VWSLFVGMRADLARTQSPGSLGPRAYPASGVP-----WQRRLAMTLSTPDRRAVEKFLQA 584
            W L   MR+    +    +L  RA P  G       W++RL   ++ P R  V +F+ A
Sbjct: 495 AWGL---MRSFADESHRKRALQYRASPLVGASRHHQGWRQRLGQAVNFPVRDQVYRFMDA 551

Query: 585 SVLPALEAVARELTRRSRPASVGRDAETGALTLTVPAEGHRDFVYGVQMSEHKLPAFTAY 644
            V PA+EAVA +L  R     V    E G + L+V     +DF+Y V ++ +++P+F ++
Sbjct: 552 DVKPAMEAVAEQL--RGDGWEVSTRLEAGDMELSVNHGDQQDFLYRVILTGYRVPSFASH 609

Query: 645 DATVADVRYEARTFFSDGSRGYDIMGMADNQIINDVLFQFERYTGFV 691
            A  +   Y A     +GS+ YD++  +  QIIND++ Q+ER+  F+
Sbjct: 610 QARNSRY-YRAEVHLYEGSQDYDLVDYSREQIINDIISQYERHVQFL 655


Lambda     K      H
   0.326    0.139    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1141
Number of extensions: 69
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 706
Length of database: 659
Length adjustment: 39
Effective length of query: 667
Effective length of database: 620
Effective search space:   413540
Effective search space used:   413540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources