Alignments for a candidate for gguA in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for gguA in Pseudarthrobacter sulfonivorans Ar51

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate WP_058930469.1 AU252_RS09320 sugar ABC transporter ATP-binding protein

Query= TCDB::O05176
         (512 letters)



>NCBI__GCF_001484605.1:WP_058930469.1
          Length = 509

 Score =  590 bits (1520), Expect = e-173
 Identities = 300/509 (58%), Positives = 386/509 (75%)

Query: 1   MANTILEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTY 60
           M   IL+MR ITKTFPGVKAL++V L V  GE+HA+ GENGAGKSTLMKVLSGVYP  ++
Sbjct: 1   MNTPILQMRGITKTFPGVKALQDVTLDVNRGEVHAICGENGAGKSTLMKVLSGVYPHNSF 60

Query: 61  EGEIHYEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASNGVISWQQT 120
           +G+I +E    NF +INDSE  GI+IIHQELAL P LSIAENI+LGNE A NG + W++T
Sbjct: 61  DGDILFENEPCNFSSINDSEKRGIVIIHQELALSPYLSIAENIYLGNEQAKNGWVDWRKT 120

Query: 121 FNRTRELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDSE 180
                +LL +VGL E+P T +  I VGKQQLVEIAKALSK VKLLILDEPTA+LN+ DS 
Sbjct: 121 NLEAAKLLARVGLSENPITPVQHISVGKQQLVEIAKALSKEVKLLILDEPTAALNDEDSG 180

Query: 181 ALLNLLMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIRN 240
            LL+L++  + QG+TSIII+HKLNE+R+VAD +T++RDG T++TL   + +I+++ IIR 
Sbjct: 181 HLLDLILHLKGQGITSIIISHKLNEIRRVADAVTIIRDGKTIETLRLDEGQITQERIIRG 240

Query: 241 MVGRDLEDRYPPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLMG 300
           MVGRDLE  YP RD  IGE +L +++W+  H Q   R V+H+ ++ VRKGEVVG+AGLMG
Sbjct: 241 MVGRDLESLYPDRDPKIGEEVLRIEDWSVRHPQDHTRMVVHNASLNVRKGEVVGLAGLMG 300

Query: 301 AGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAGLAYVTEDRKHLGLVLNDN 360
           AGRTE AMSVFG++YG  ++G V   GK ++ STV +AI  G+AY TEDRKH GL L ++
Sbjct: 301 AGRTELAMSVFGRTYGTAVSGKVYKYGKEINTSTVSQAIGHGIAYATEDRKHYGLNLIED 360

Query: 361 ILHNTTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGGNQQKVVLS 420
           I  N ++A L  + K   +D  +E  VA+ +RT + I++  +   T  LSGGNQQKVVLS
Sbjct: 361 IQRNISMAALRKLVKGGWVDKNRETVVANGYRTSMNIKAPSVAAITGKLSGGNQQKVVLS 420

Query: 421 KWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELLGNCDRIYV 480
           KW+FS+PDVLILDEPTRGIDVGAK+EIYTII +LAA+GK V++ISSE+PELLG CDRIY 
Sbjct: 421 KWMFSDPDVLILDEPTRGIDVGAKFEIYTIIARLAAEGKAVIVISSELPELLGICDRIYT 480

Query: 481 MNEGRIVAELPKGEASQESIMRAIMRSGE 509
           ++ G I  E+P  EASQE++M  + +  E
Sbjct: 481 LSAGHITGEVPIAEASQETLMHYMTKEKE 509


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 724
Number of extensions: 26
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 512
Length of database: 509
Length adjustment: 35
Effective length of query: 477
Effective length of database: 474
Effective search space:   226098
Effective search space used:   226098
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources