Alignments for a candidate for aldA in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for aldA in Pseudarthrobacter sulfonivorans Ar51

Align Lactaldehyde dehydrogenase (EC 1.2.1.22); Rhamnulose-1-phosphate aldolase (EC 4.1.2.19) (characterized)
to candidate WP_058931373.1 AU252_RS14785 bifunctional rhamnulose-1-phosphate aldolase/short-chain dehydrogenase

Query= reanno::Cola:Echvi_1572
         (702 letters)



>NCBI__GCF_001484605.1:WP_058931373.1
          Length = 688

 Score =  650 bits (1676), Expect = 0.0
 Identities = 334/676 (49%), Positives = 448/676 (66%), Gaps = 10/676 (1%)

Query: 31  LIYRSNILGADLRITNYGGGNTSCKTTEVDPLTKEETEVMWVKGSGGDIGTLKRSGLAGL 90
           LI RSN LGAD R TN+ GGNTS K  E DP+T E+ +++WVKGSGGD+GTL    LA L
Sbjct: 19  LIARSNRLGADKRNTNFAGGNTSAKGAEKDPVTGEDVQLLWVKGSGGDLGTLTAGNLAVL 78

Query: 91  YVEKLHSLKNVYRGLEFEDEMVGLFNHCIYDLDSKAPSIDTPLHAFLPFKHIDHLHPDAA 150
            +++L +LK VY G+E EDEMV  F++C++     APSIDT +H  +   H+DHLHPD+ 
Sbjct: 79  RLDRLQALKAVYPGVEREDEMVAAFDYCLHGKGGAAPSIDTAMHGLVDAAHVDHLHPDSG 138

Query: 151 IAIAASKDGEKITEELFEGQIAWVPWQRPGFDLALQLEKALNENPGIRGIMLGGHGLFTW 210
           IAIA + DGE +T ++F  ++ WVPW+RPGF L L +    + NP   G +LGGHG+  W
Sbjct: 139 IAIATAVDGEALTSKIFGDKVLWVPWRRPGFQLGLDIAAIKDANPQAIGTILGGHGITAW 198

Query: 211 GDTAYECYINSLEVIDKASEYLEQNYGKDRPVFGGQKIESLAP---EQRQEQASIIAPVL 267
           G T+ E   NSL +I++A +++E+N GK  P   G ++    P    +R+ +A+ +APV+
Sbjct: 199 GATSEEAEQNSLWIIEQAEKFIEEN-GKAEPF--GPRLAGYGPLPEAERRAKAAALAPVI 255

Query: 268 RGLASGYNRMVGHFTDDERVLQFANSHDLEKLAPLGTSCPDHFLRTKIRPLVLDFPADVD 327
           RGLAS     +GHF+DD  VL F  + +  +L  LGTSCPDHFLRTK++PL+LD PAD  
Sbjct: 256 RGLASTDKPQLGHFSDDAVVLDFLAAAEHPRLGALGTSCPDHFLRTKVKPLILDLPADAS 315

Query: 328 LSKADEIKEKLDKDFEEYRAYYKKYYEDHKRDNSPAMRDPNPVVIIWPGVGMFSYAKNKQ 387
           +   ++   +L +    YR  Y+ YY+ H   +SPA+R  +P +++ PGVGMFSY  NKQ
Sbjct: 316 I---EDSIARLKELHTAYREDYQAYYDRHAVPDSPALRGADPAIVLVPGVGMFSYGANKQ 372

Query: 388 TARVASEFYINAINVMRGAEAVSEYVALPLQEAFDIEYWLLEEAKLQRMPKEQPLSRKVA 447
           TARVA EFY+NAINVMRGAEA+S Y  +   E F IEYW LEEAKL R+PK +  + ++A
Sbjct: 373 TARVAGEFYLNAINVMRGAEAISTYAPIEESEKFRIEYWSLEEAKLARLPKPKSHASRIA 432

Query: 448 LVTGGAGGIGKAIADKLASEGACVFITDINQERLDGAVATY-SKDVGGGAVMDVTKGDDI 506
           LVTG A GIGKAIA +LAS+GACV I D+N E      A     DV  G   DVT    I
Sbjct: 433 LVTGAASGIGKAIATRLASDGACVVIADLNLENAQAVAAELGGADVAIGVQADVTDEAQI 492

Query: 507 IKAYKAAALKFGGVDIIVNCAGLAISKPIEQTSEQDWDLLQDILVKGQFAVSKAGVETLR 566
             A + A L FGG+D++VN AGL+ISKP+ +T+E+DWDL  +++ KG F VSKA  + + 
Sbjct: 493 AAAIQEAVLAFGGLDLVVNNAGLSISKPLLETTEKDWDLQHNVMAKGSFLVSKAAAKVMI 552

Query: 567 AQNLGGDIINIASKNALVSGPNNVGYGTAKAAQVHMSRLLAAELGKDKIRVNVVNPDAVI 626
           AQ++GGDII I+SKN++ +GPNN+ Y   KA Q H  RLLAAELG+  IRVN +NPD V+
Sbjct: 553 AQDMGGDIIYISSKNSVFAGPNNIAYSATKADQAHQVRLLAAELGEYGIRVNGINPDGVV 612

Query: 627 EGSKIWEGEWAKGRAKAYGITVEELPAFYAKRTILNEIIGVDDIANGVFAFVGGHLSKCT 686
            GS I+ G W   RA  YG+  +EL  +YA+RT+L   +  + +AN         LS  T
Sbjct: 613 RGSGIFAGGWGAKRAAVYGVDEQELGKYYAQRTLLKREVLPEHVANAAAVLTSAELSHTT 672

Query: 687 GNILNVDGGVAAAFVR 702
           G  + VD GVAAAF+R
Sbjct: 673 GLHIPVDAGVAAAFLR 688


Lambda     K      H
   0.317    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1166
Number of extensions: 47
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 702
Length of database: 688
Length adjustment: 39
Effective length of query: 663
Effective length of database: 649
Effective search space:   430287
Effective search space used:   430287
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources