Align actP-like component of L-lactate and L-malate uptake system (characterized)
to candidate WP_068166469.1 HTA01S_RS00855 cation acetate symporter
Query= reanno::PV4:5209923 (572 letters) >NCBI__GCF_001592305.1:WP_068166469.1 Length = 600 Score = 716 bits (1849), Expect = 0.0 Identities = 372/606 (61%), Positives = 456/606 (75%), Gaps = 40/606 (6%) Query: 1 MDVQTLTYLIVGFTFALYIGIAIWSRAGSTKEFYVAGGGVHPVMNGMATAADWMSAASFI 60 MD+QT TYL VG +FALY+GIA W+RAGS EFYVAGGGVHP+ NGMATAADWMSAASFI Sbjct: 1 MDLQTTTYLFVGASFALYLGIAFWARAGSVSEFYVAGGGVHPITNGMATAADWMSAASFI 60 Query: 61 SLAGIVSFVGYDGSVYLMGWTGGYVLLALCMAPYLRKFGKFTVPDFIGDRYYSQAARTVA 120 S+AG++S +GY G+V+LMGWTGGYVLLA+ +APYLRKFGKFTVP FIGDR+YS+ A TVA Sbjct: 61 SMAGLISNMGYGGAVFLMGWTGGYVLLAMLLAPYLRKFGKFTVPQFIGDRFYSKTASTVA 120 Query: 121 VVCAIFICFTYIAGQMRGVGVVFSRFLEVEVDTGVYIGMAVVFFYAVLGGMKGITYTQVA 180 V+C + TYI GQM GVGV FSRFL V DTG+YIG A+VFFYAV GGMKGITYTQVA Sbjct: 121 VICLLTASTTYIIGQMTGVGVAFSRFLNVSSDTGIYIGQAIVFFYAVFGGMKGITYTQVA 180 Query: 181 QYCVLIFAFMVPAIFISVMMTGHILPQLGFGAELVDAAGNNTGVYLLEKLDGLSAQLGFS 240 QY VLIFA+++PAIFIS+ +TG LPQLG GA+L AG +T LL KLD + LGF+ Sbjct: 181 QYIVLIFAYIIPAIFISLNLTGSFLPQLGLGADL---AGTDTA--LLAKLDQVVTDLGFN 235 Query: 241 QYTEGSKG-MIDVFFITGALMFGTAGLPHVIVRFFTVPKVKDARVSAGWALVFIAIMYTT 299 QYT S G M+++FF T +LM GTAGLPHVIVRFFTVPKV DAR SAGWALVFIA++YTT Sbjct: 236 QYTTTSAGSMLNMFFYTVSLMIGTAGLPHVIVRFFTVPKVADARASAGWALVFIALLYTT 295 Query: 300 IPALAAFSRVNMIETIN-GPESTGVAY--------ETAPDWIKNWEKTGLIKWDDKNNDG 350 PA+ A +++N+ T+N S G Y E PDW+K WEKTGL+KW+DKN DG Sbjct: 296 APAVGAMAKLNLHSTVNTAVMSNGDLYATESSLKNEDRPDWMKRWEKTGLLKWEDKNADG 355 Query: 351 KIYYAKGET----------------NEMKIDRDIMVLATPEIANLPAWVIALVAAGGLAA 394 +I Y T NE+ ++ DI+VLA PEIA LP WVIALVAAGG+AA Sbjct: 356 RIQYYNDATKNEIAKSKATAAGWKGNELTVNADIIVLANPEIALLPNWVIALVAAGGIAA 415 Query: 395 ALSTSAGLLLVISTSVSHDLLKKNFMPDISDKQELLYARIAAALGIVMAGYFGINPPGFV 454 ALST+AGLL+ IS++VSHDL+K F P+IS+K ELL +IA A+ IV+AG+ G+NPPGF Sbjct: 416 ALSTAAGLLMAISSAVSHDLIKNVFNPNISEKGELLAGKIAMAVAIVLAGWLGLNPPGFA 475 Query: 455 AAVVAIAFGLAASSLFPAIIMGIFSRTMNKEGAIAGMVIGLLFSASYIIYFK---FVNPG 511 A VA+AFG+AASS+FPAI+MGIFSRTM+ +GAIAGM+ GLL + Y+ K F+ Sbjct: 476 AGTVALAFGIAASSIFPAIMMGIFSRTMSDKGAIAGMLSGLLVTLFYVFAHKGLFFIKGT 535 Query: 512 D-----NNASNWLFGISPEGIGMLGMIINFAVAFIVSKVTAAVPQNVVDMVESIRFPKGA 566 D + W FGI+PE +G +G I+NF VAF+V+K+TA P+++ ++VESIR PKGA Sbjct: 536 DFVDMVGGVNGW-FGITPEAVGTVGAIVNFTVAFLVNKLTAPAPKHIQELVESIRVPKGA 594 Query: 567 GEAHDH 572 G A H Sbjct: 595 GAATGH 600 Lambda K H 0.326 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1095 Number of extensions: 50 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 572 Length of database: 600 Length adjustment: 37 Effective length of query: 535 Effective length of database: 563 Effective search space: 301205 Effective search space used: 301205 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory