Align Beta-ketothiolase BktB; Acetyl-CoA acetyltransferase; Acetyl-CoA acyltransferase; EC 2.3.1.16; EC 2.3.1.9 (characterized)
to candidate WP_068166893.1 HTA01S_RS02090 acetyl-CoA C-acyltransferase
Query= SwissProt::Q0KBP1 (394 letters) >NCBI__GCF_001592305.1:WP_068166893.1 Length = 394 Score = 592 bits (1526), Expect = e-174 Identities = 293/394 (74%), Positives = 338/394 (85%) Query: 1 MTREVVVVSGVRTAIGTFGGSLKDVAPAELGALVVREALARAQVSGDDVGHVVFGNVIQT 60 M+REVV+VS RTAIGTFGGSLKDV P ELG LVV+E LARAQ+ G DVGHVVFG+V+ T Sbjct: 1 MSREVVIVSAARTAIGTFGGSLKDVPPTELGTLVVKETLARAQLDGKDVGHVVFGHVVNT 60 Query: 61 EPRDMYLGRVAAVNGGVTINAPALTVNRLCGSGLQAIVSAAQTILLGDTDVAIGGGAESM 120 EP+DMYL RVAA+NGG PA VNRLCGSGLQAIVSA+Q+ILLGDTD+AIGGGAE M Sbjct: 61 EPKDMYLSRVAAINGGCAEGTPAFNVNRLCGSGLQAIVSASQSILLGDTDIAIGGGAECM 120 Query: 121 SRAPYLAPAARWGARMGDAGLVDMMLGALHDPFHRIHMGVTAENVAKEYDISRAQQDEAA 180 SRAP+ + RWGARMGD +VDMM+GALHDPFH IHMGVTAEN+A ++ ISR QD A Sbjct: 121 SRAPFASLNMRWGARMGDTKMVDMMVGALHDPFHTIHMGVTAENIAAKWGISREDQDRLA 180 Query: 181 LESHRRASAAIKAGYFKDQIVPVVSKGRKGDVTFDTDEHVRHDATIDDMTKLRPVFVKEN 240 +ESH RA A + G FKDQIVPV+ K RKG+V + TDEH R + ++++ KL+PVFVK+N Sbjct: 181 VESHNRAQRATEEGRFKDQIVPVMLKSRKGEVAYATDEHFRPNCGMEELAKLKPVFVKDN 240 Query: 241 GTVTAGNASGLNDAAAAVVMMERAEAERRGLKPLARLVSYGHAGVDPKAMGIGPVPATKI 300 GTVTAGNASG+NDAAAAVV+ME A A+ RGL PLARLV+Y HAGVDPK MGIGPVPA+K+ Sbjct: 241 GTVTAGNASGINDAAAAVVLMEAATAKARGLAPLARLVAYAHAGVDPKYMGIGPVPASKL 300 Query: 301 ALERAGLQVSDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALIT 360 AL++AGL V+DLDVIEANEAFAAQACAV++ LGLDPAKVNPNGSGISLGHPIGATGALIT Sbjct: 301 ALQKAGLTVNDLDVIEANEAFAAQACAVSRDLGLDPAKVNPNGSGISLGHPIGATGALIT 360 Query: 361 VKALHELNRVQGRYALVTMCIGGGQGIAAIFERI 394 VKAL+EL R+ GRYALVTMCIGGGQGIAA+FER+ Sbjct: 361 VKALYELQRINGRYALVTMCIGGGQGIAAVFERL 394 Lambda K H 0.318 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 544 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 394 Length of database: 394 Length adjustment: 31 Effective length of query: 363 Effective length of database: 363 Effective search space: 131769 Effective search space used: 131769 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_068166893.1 HTA01S_RS02090 (acetyl-CoA C-acyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.20932.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-142 460.0 4.2 3.4e-142 459.9 4.2 1.0 1 lcl|NCBI__GCF_001592305.1:WP_068166893.1 HTA01S_RS02090 acetyl-CoA C-acyl Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001592305.1:WP_068166893.1 HTA01S_RS02090 acetyl-CoA C-acyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 459.9 4.2 3.4e-142 3.4e-142 1 385 [] 7 392 .. 7 392 .. 0.98 Alignments for each domain: == domain 1 score: 459.9 bits; conditional E-value: 3.4e-142 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageq.aniaReaalaa 68 iv+a+Rt+ig++ggslk++++ +L++ v+ke l+ra+ld +++ +v++G+v+++ + + + R aa++ lcl|NCBI__GCF_001592305.1:WP_068166893.1 7 IVSAARTAIGTFGGSLKDVPPTELGTLVVKETLARAQLDGKDVGHVVFGHVVNTEPKdMYLSRVAAING 75 8****************************************************99998*********** PP TIGR01930 69 glpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakle 137 g+ e +pa++vnr+C+SglqA+ +a q+i G+ d+++ GG+E+mSr+p+ + + r+++++g++k+ lcl|NCBI__GCF_001592305.1:WP_068166893.1 76 GCAEGTPAFNVNRLCGSGLQAIVSASQSILLGDTDIAIGGGAECMSRAPFASLNM--RWGARMGDTKMV 142 *************************************************998886..8*********** PP TIGR01930 138 dqllkdl..vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..k 202 d+++ l + + ++mg+tAen+a+k+gisRe+qD++a++Sh++a++A+eeg+fkd+ivpv +k + + lcl|NCBI__GCF_001592305.1:WP_068166893.1 143 DMMVGALhdPFHTIHMGVTAENIAAKWGISREDQDRLAVESHNRAQRATEEGRFKDQIVPVMLKSRkgE 211 *9999889999****************************************************999999 PP TIGR01930 203 kvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivs 271 + +++De+ rpn +e+LakLkp+f + +g tvtAgN+s++nD+Aaa++lm+ ++ak+ gl plar+v+ lcl|NCBI__GCF_001592305.1:WP_068166893.1 212 VAYATDEHFRPNCGMEELAKLKPVFVKDNG-TVTAGNASGINDAAAAVVLMEAATAKARGLAPLARLVA 279 999***************************.6************************************* PP TIGR01930 272 aavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAiA 340 +a+agvdp++mg+gpvpA++ aL+kagl+++d+d++E nEAFAaq+ av ++lg ld++kvN nG+ i+ lcl|NCBI__GCF_001592305.1:WP_068166893.1 280 YAHAGVDPKYMGIGPVPASKLALQKAGLTVNDLDVIEANEAFAAQACAVSRDLG-LDPAKVNPNGSGIS 347 ******************************************************.99************ PP TIGR01930 341 lGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 lGHP+Ga+Ga i+++ l+eL++ +++y+l+t+C+ggGqG+A+++e lcl|NCBI__GCF_001592305.1:WP_068166893.1 348 LGHPIGATGALITVKALYELQRINGRYALVTMCIGGGQGIAAVFE 392 *******************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (394 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 11.40 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory