Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_068168254.1 HTA01S_RS06610 acetate--CoA ligase
Query= SwissProt::P39062 (572 letters) >NCBI__GCF_001592305.1:WP_068168254.1 Length = 587 Score = 525 bits (1351), Expect = e-153 Identities = 270/569 (47%), Positives = 362/569 (63%), Gaps = 14/569 (2%) Query: 14 NLKNYEETYRHFDWAEAEKHFSWHETGK-LNAAYEAIDRHAESFRKNKVALYY--KDAKR 70 NL +Y+ T F W EAE+ G LN A+EA+DRH R +K A+ + K +R Sbjct: 17 NLSDYDATVAGFSWQEAERQLDGLPGGAGLNIAHEAVDRHVLHGRGHKTAIRWLGKSGER 76 Query: 71 DEKYTFKEMKEESNRAGNVLRRYGNVEKGDRVFIFMPRSPELYFIMLGAIKIGAIAGPLF 130 E +++ ++ +N+ + L G V G+RVFI M R PELY +LGA+K + PLF Sbjct: 77 RE-FSYLDLARATNQFAHALEHLG-VRPGERVFILMGRLPELYVALLGALKARCVVTPLF 134 Query: 131 EAFMEGAVKDRLENSEAKVVVTTPELLERIPVD---KLPHLQHVFVVGGEAESGTNIINY 187 AF + R E +A+V+VTTPEL +R D +LP L+HV V G + + Sbjct: 135 SAFGPEPIATRAEMGDARVLVTTPELYQRKVQDLRERLPGLRHVIVAGAVPSDEGGVHPW 194 Query: 188 DEAAKQESTRLDIEWMDKKDGFLLHYTSGSTGTPKGVLHVHEAMIQQYQTGKWVLDLKEE 247 ST I D + LLH+TSG+TG PKG +HVH A++ TG++ LDL ++ Sbjct: 195 GALVSPASTTYTIAPTDPESMALLHFTSGTTGRPKGAVHVHAAVLAHMVTGRYALDLHDD 254 Query: 248 DIYWCTADPGWVTGTVYGIFAPWLNGATNVIVGGRFSPESWYGTIEQLGVNVWYSAPTAF 307 D++WCTADPGWVTGT YGI AP + G TNV+V F ++WYG +E+ V++WY+APTA Sbjct: 255 DVFWCTADPGWVTGTSYGIIAPLVCGVTNVVVEAEFDAQTWYGVLERERVSIWYTAPTAI 314 Query: 308 RMLMGAGDEMAAKYDLTSLRHVLSVGEPLNPEVIRWGHKVFNKRIHDTWWMTETGSQLIC 367 RM+M G E DL++LR + SVGEPLNPE + WG + F HD WW TETG ++ Sbjct: 315 RMMMKLGAEALKGVDLSALRFMASVGEPLNPEAVVWGLEAFGLPFHDNWWQTETGGIMVS 374 Query: 368 NYPCMDIKPGSMGKPIPGVEAAIVDNQGNE-----LPPYRMGNLAIKKGWPSMMHTIWNN 422 NY MDI+PGSMGKP+PG+ AA+V G+ P G LA++ WPSMM + Sbjct: 375 NYLAMDIRPGSMGKPLPGIVAAVVRRTGSGHVEAITAPDVDGELALRAPWPSMMRGYLHE 434 Query: 423 PEKYESYFMPGGWYVSGDSAYMDEEGYFWFQGRVDDVIMTSGERVGPFEVESKLVEHPAI 482 E+Y F+ GWY++GD D EGY+WF GR DDVI +SG +GPFEVES L+EHPA+ Sbjct: 435 DERYRKCFV-DGWYLTGDLVRRDAEGYYWFVGRADDVIKSSGHLIGPFEVESALMEHPAV 493 Query: 483 AEAGVIGKPDPVRGEIIKAFIALREGFEPSDKLKEEIRLFVKQGLAAHAAPREIEFKDKL 542 AEAGVIG PDP+ GE++KAF+AL+ G E S+ L+ E+ ++ L A AP++I+F+D L Sbjct: 494 AEAGVIGIPDPMAGEVVKAFVALKPGHEASEALRRELLGHARKRLGAAVAPKQIDFRDNL 553 Query: 543 PKTRSGKIMRRVLKAWELNLPAGDLSTME 571 PKTRSGKIMRR+LKA EL LP GDLST+E Sbjct: 554 PKTRSGKIMRRLLKARELGLPEGDLSTLE 582 Lambda K H 0.318 0.136 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1064 Number of extensions: 44 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 572 Length of database: 587 Length adjustment: 36 Effective length of query: 536 Effective length of database: 551 Effective search space: 295336 Effective search space used: 295336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory