Alignments for a candidate for ech in Hydrogenophaga taeniospiralis NBRC 102512

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Hydrogenophaga taeniospiralis NBRC 102512

Align 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized)
to candidate WP_068172592.1 HTA01S_RS13945 3-hydroxyacyl-CoA dehydrogenase

Query= BRENDA::A4YDS4
         (651 letters)



>NCBI__GCF_001592305.1:WP_068172592.1
          Length = 702

 Score =  176 bits (447), Expect = 2e-48
 Identities = 128/406 (31%), Positives = 211/406 (51%), Gaps = 40/406 (9%)

Query: 3   VTVIGSGVMGHGIAELAAIAGNEVWMNDISTEILQQAMERIKWSLSKLRESGSLKEGVEQ 62
           V VIG+G MG GI      AG  V + ++  + L + +  I+ +     + G LK    +
Sbjct: 304 VAVIGAGTMGGGITMNFLNAGIPVKVLEMKQDALDRGVATIRKNYEAQVKKGKLKADKYE 363

Query: 63  VLARIHPETDQAQALKGSDFVIEAVKEDLELKRTIFRNAEAHASPSAVLATNTSSLPISE 122
               +   T     +  +D VIEAV E++ +K  +F+  +    P A+LA+NTS+L +++
Sbjct: 364 QRMSLLSTTLSYDDIGLADLVIEAVFEEIGVKEAVFKELDRVMKPGAILASNTSTLDVNK 423

Query: 123 IASVLKSPQRVVGMHFFNPPVLMPLVEIVRGKDTSDEVVKTTAEMAKSMNKETIVVKDVP 182
           IAS  + PQ VVG+HFF+P  +M L+E+VRG+ T+ +V+ T   +AK + K  +V     
Sbjct: 424 IASFTQRPQDVVGLHFFSPANVMKLLEVVRGEKTAKDVMATVMTVAKKIKKTAVVSGVCD 483

Query: 183 GFFVNRVLLRIMEAGCYLVEKGIASIQEVDSSAIEELGFPMGVFLLADYTGLDIGYSVWK 242
           GF  NR++ +    G +L+++G  + ++VD  A+E+ G  MG F + D  G DIG+++ K
Sbjct: 484 GFIGNRMIEQYGRQGGFLLDEG-CTPEQVD-KAMEKFGMAMGPFRMGDLAGNDIGWAIRK 541

Query: 243 AVTARGFKAFP----CSSTEKLVSQGKLGVKSGSGYYQYPSPGKFVRPTLPSTS------ 292
               R ++  P      + + L  +G+ G K+G+G+Y Y  PGK  R  +P+        
Sbjct: 542 ----RRYQEKPDMKYSKTADLLCEKGRFGQKTGAGWYDY-VPGK--RDAIPNAEVVQMIE 594

Query: 293 ---KKLG------------RYLISPAVNEVSYLLREGIVGK-DDAEKGCVLGLGLP---K 333
              K LG            + L+   VNE +++L EGI  K  D +   + G G P    
Sbjct: 595 DHRKALGITPRKISDEEIVQRLVFSLVNEAAHILEEGIANKASDIDVVYIFGYGFPVYRG 654

Query: 334 GILSYADEIGIDVVVNTLEEMRQTSGMD--HYSPDPLLLSMVKEGK 377
           G L+YA+E+G+  VV  ++   +    D   + P PLL  + +EGK
Sbjct: 655 GPLNYANEVGLFNVVQAMKRFAKNPLDDAKFWQPAPLLARLAEEGK 700



 Score =  114 bits (285), Expect = 1e-29
 Identities = 71/201 (35%), Positives = 104/201 (51%), Gaps = 10/201 (4%)

Query: 398 TIVVRVEPPLAWIVLNRPTRYNAINGDMIREINQALDSLEEREDVRVIAITGQGRVFSAG 457
           T   +V   +A I LN P   N +     + I   L+  E    V+ I +TG G+ FS G
Sbjct: 2   TAEYQVHGDVAVITLNNPP-VNGLGLSTRQAITAGLEKAEADPAVKAIVLTGAGKAFSGG 60

Query: 458 ADVTEFGSLTPVKAMIASRKFHEVFMKIQFLTKPVIAVINGLALGGGMELALSADFRVAS 517
           AD+ EFGS       I       V + ++  +KPV+A ++ + +GGG+ELAL A +R+A+
Sbjct: 61  ADIREFGS----PKAIQEPNLLSVILAVENTSKPVVAAVHSVCMGGGLELALGAHYRIAA 116

Query: 518 KTAEMGQPEINLGLIPGGGGTQRLSRLSG-RKGLELVLTGRRVKAEEAYRLG----IVEF 572
               +  PE+ LGLIPG GGTQRL R+ G    L ++++G  VK+E   +L       + 
Sbjct: 117 PGCNVALPEVKLGLIPGAGGTQRLPRVLGVEAALNMIVSGEPVKSEMLAQLPGQKLFDKL 176

Query: 573 LAEPEELESEVRKLANAIAEK 593
            A PE L  E    A  +A K
Sbjct: 177 AASPESLAEEALAFAREVAAK 197


Lambda     K      H
   0.316    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 932
Number of extensions: 62
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 651
Length of database: 702
Length adjustment: 39
Effective length of query: 612
Effective length of database: 663
Effective search space:   405756
Effective search space used:   405756
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources