Align NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate WP_068173839.1 HTA01S_RS16510 ABC transporter ATP-binding protein
Query= TCDB::Q7A2H0 (260 letters) >NCBI__GCF_001592305.1:WP_068173839.1 Length = 256 Score = 198 bits (504), Expect = 8e-56 Identities = 107/252 (42%), Positives = 160/252 (63%), Gaps = 7/252 (2%) Query: 11 LLAASGLCKSFGGIKAVQEARIEVAQGSITGLIGPNGAGKTTLFNLLSNFIRPDKGRVIF 70 +L + + K FGG++A+ + IE+ +G + GLIGPNGAGKTT FN+L+ PD G Sbjct: 5 ILKVANVSKRFGGLQALSDVGIEIKRGQVYGLIGPNGAGKTTFFNVLTGLYTPDSGSFEL 64 Query: 71 DGEPIQQLQPHQIAQQGMVRTFQVARTLSRLSVLENMLLAAQKQTGENFWQVQLQPQVVV 130 G+P H++A+ G+ RTFQ R + ++ LEN+++ +T L+ Sbjct: 65 AGKPYTPTAVHEVAKAGIARTFQNIRLFAEMTALENVMVGRHVRTHSGLIGAVLRTPGFK 124 Query: 131 KEEKQLQEQAMFLLESVGLAKKAYEYAGGLSGGQRKLLEMGRALMTNPKLILLDEPAAGV 190 +EE ++ E+A LLE VG+AK A + LS G ++ LE+ RAL T+P+LI LDEPAAG+ Sbjct: 125 REEAEITERARELLEYVGIAKFADFKSRTLSYGDQRRLEIARALATDPQLIALDEPAAGM 184 Query: 191 NPR---LIDDICDRILTWNRQDGMTFLIIEHNMDVIMSLCDRVWVLAEGQNLADGTPAEI 247 N L+ ++ DRI R+D T L+IEH++ ++M LCDRV VL G+ L+ GTPAE+ Sbjct: 185 NATEKVLLRELIDRI----RKDNRTILLIEHDVKLVMGLCDRVTVLDYGKQLSSGTPAEV 240 Query: 248 QTNSQVLEAYLG 259 Q++ +V+EAYLG Sbjct: 241 QSDPKVIEAYLG 252 Lambda K H 0.319 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 157 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 260 Length of database: 256 Length adjustment: 24 Effective length of query: 236 Effective length of database: 232 Effective search space: 54752 Effective search space used: 54752 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory