Alignments for a candidate for mcm-large in Malonomonas rubra DSM 5091

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Malonomonas rubra DSM 5091

Align methylmalonyl-CoA mutase (EC 5.4.99.2) (characterized)
to candidate WP_072907423.1 BUB13_RS07410 methylmalonyl-CoA mutase

Query= BRENDA::P11653
         (728 letters)



>NCBI__GCF_900142125.1:WP_072907423.1
          Length = 712

 Score =  854 bits (2207), Expect = 0.0
 Identities = 435/678 (64%), Positives = 521/678 (76%), Gaps = 5/678 (0%)

Query: 38  WETAEQIPVGTLFNEDVYKDMDWLDTYAGIPPFVHGPYATMYAFRPWTIRQYAGFSTAKE 97
           W+T E I V  L+     + ++  DT  G+ PFV GP ATMYA RPWT+RQYAGFSTA+E
Sbjct: 30  WDTPEGISVKPLYTAADIEGLETADTMPGLAPFVRGPMATMYAGRPWTVRQYAGFSTAEE 89

Query: 98  SNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVAGDVGMAGVAIDSIYDMRELFAGIP 157
           SNAFY+RNLAAGQ+GLSVAFDL THRGYDSD+PRV GDVG AGVAIDS+ DM+ LF+ IP
Sbjct: 90  SNAFYKRNLAAGQQGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVEDMKILFSDIP 149

Query: 158 LDQMSVSMTMNGAVLPILALYVVTAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPQPS 217
           LD++SVSMTMNGAVLPI+A Y++ AEEQGV  ++LAGTIQNDILKEFMVRNTYIYPP PS
Sbjct: 150 LDKVSVSMTMNGAVLPIMANYIIAAEEQGVSEDKLAGTIQNDILKEFMVRNTYIYPPAPS 209

Query: 218 MRIISEIFAYTSANMPKWNSISISGYHMQEAGATADIEMAYTLADGVDYIRAGESVGLNV 277
           M+II +I  YTS  MPK+NSISISGYH+QEAGA   +E+A+TLADG++Y+R   + GL+V
Sbjct: 210 MKIIGDIIEYTSQKMPKFNSISISGYHIQEAGANNALELAFTLADGLEYVRQAVAKGLDV 269

Query: 278 DQFAPRLSFFWGIGMNFFMEVAKLRAARMLWAKLVHQFGPKNPKSMSLRTHSQTSGWSLT 337
           D FAPRLSFF+ IGMNFFME +KLRAAR LWAK++ QF PKNPKS  LRTH QTSGWSLT
Sbjct: 270 DAFAPRLSFFFAIGMNFFMEASKLRAARFLWAKMMKQFNPKNPKSSMLRTHCQTSGWSLT 329

Query: 338 AQDVYNNVVRTCIEAMAATQGHTQSLHTNSLDEAIALPTDFSARIARNTQLFLQQESGTT 397
            QD YNNV+RT +EA+AA  G TQSLHTN+LDEAIALPTD SARIARNTQL +Q+ESG  
Sbjct: 330 EQDPYNNVIRTTLEALAAVLGGTQSLHTNALDEAIALPTDHSARIARNTQLVIQEESGVC 389

Query: 398 RVIDPWSGSAYVEELTWDLARKAWGHIQEVEKVGGMAKAIEKGIPKMRIEEAAARTQARI 457
            V+DP  GS YVE LT  L  +A   + E+E++GGM KAIE G+PK+RIEE+AA+ QA I
Sbjct: 390 NVVDPLGGSYYVESLTNSLIEEASKILDEIEELGGMTKAIESGMPKLRIEESAAKKQASI 449

Query: 458 DSGRQPLIGVNKYRLEHEPPLDVLKVDNSTVLAEQKAKLVKLRAERDPEKVKAALDKITW 517
           DSGR  ++GVNKY+L  E  ++VL VDN+ V   Q A+L K+RA+RD    + ALD IT 
Sbjct: 450 DSGRDVIVGVNKYKLAEEDEIEVLDVDNAAVRESQIARLNKMRADRDEAACQQALDAITA 509

Query: 518 AAGNPDDKDPDRNLLKLCIDAGRAMATVGEMSDALEKVFGRYTAQIRTISGVYSKEVKNT 577
           A      +    NLL L + A R  A+VGE+SDA+EKVFGR+ A+I+ +SG Y   V   
Sbjct: 510 AC-----ESGAGNLLDLSVKAARLRASVGEISDAMEKVFGRHKAEIKLVSGAYGSVVNED 564

Query: 578 PEVEEARELVEEFEQAEGRRPRILLAKMGQDGHDRGQKVIATAYADLGFDVDVGPLFQTP 637
            +  E ++ ++ F   EGRRPRIL+AKMGQDGHDRG KV+A+AYAD GFDVDVGPLFQTP
Sbjct: 565 NDFNEVKKRIDAFAAKEGRRPRILVAKMGQDGHDRGAKVVASAYADAGFDVDVGPLFQTP 624

Query: 638 EETARQAVEADVHVVGVSSLAGGHLTLVPALRKELDKLGRPDILITVGGVIPEQDFDELR 697
           EE A+ AVE DVHVVGVSSLA GH TLVP L  EL KLG  DI +  GGVIP QD+D L 
Sbjct: 625 EEAAKMAVENDVHVVGVSSLAAGHKTLVPQLVAELKKLGADDIAVVCGGVIPRQDYDALY 684

Query: 698 KDGAVEIYTPGTVIPESA 715
           + GA +I+ PGT I  SA
Sbjct: 685 EAGAAKIFGPGTPITVSA 702


Lambda     K      H
   0.317    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1307
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 728
Length of database: 712
Length adjustment: 40
Effective length of query: 688
Effective length of database: 672
Effective search space:   462336
Effective search space used:   462336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources