Align acetyl-CoA C-acetyltransferase (EC 2.3.1.9) (characterized)
to candidate WP_073037060.1 BUB04_RS03720 acetyl-CoA C-acetyltransferase
Query= BRENDA::P45359 (392 letters) >NCBI__GCF_900129305.1:WP_073037060.1 Length = 392 Score = 551 bits (1421), Expect = e-162 Identities = 275/392 (70%), Positives = 330/392 (84%), Gaps = 1/392 (0%) Query: 1 MKEVVIASAVRTAIGSYGKSLKDVPAVDLGATAIKEAVKKAGIKPEDVNEVILGNVLQAG 60 M+ VI SAVRT +GS+GKSL +PAV LG TA++EA+K+ + P+ V+EVILGNVLQA Sbjct: 1 MQRAVIVSAVRTPVGSFGKSLAAIPAVALGVTAVREALKRIALSPDQVDEVILGNVLQAA 60 Query: 61 LGQNPARQASFKAGLPVEIPAMTINKVCGSGLRTVSLAAQIIKAGDADVIIAGGMENMSR 120 GQNPARQ + AG+P E+PA TINKVC SGL++V LAAQ + AGDA+V++AGG+ENMS Sbjct: 61 QGQNPARQVAVHAGIPHEVPAFTINKVCASGLKSVVLAAQAVLAGDAEVVVAGGIENMSA 120 Query: 121 APYLANNARWGYRMGNAKFVDEMITDGLWDAFNDYHMGITAENIAERWNISREEQDEFAL 180 APY ARWG RMG+ + VD MI DGLWD FN YHMGITAEN+AER+ ISREEQD FAL Sbjct: 121 APYALPKARWGQRMGHGELVDLMILDGLWDIFNGYHMGITAENVAERFGISREEQDAFAL 180 Query: 181 ASQKKAEEAIKSGQFKDEIVPVVIKGRKGETVV-DTDEHPRFGSTIEGLAKLKPAFKKDG 239 ASQ+KAE AIK G+FKDEIVPV I RKG+ V+ DTDEHPRFG+T+E LAKLKPAFKKDG Sbjct: 181 ASQQKAETAIKEGRFKDEIVPVEIPQRKGDPVLFDTDEHPRFGTTLEALAKLKPAFKKDG 240 Query: 240 TVTAGNASGLNDCAAVLVIMSAEKAKELGVKPLAKIVSYGSAGVDPAIMGYGPFYATKAA 299 TVTAGNASG+ND AA+LV+MS +KA+ELG+ PLA I SY SAGVDPAIMG GP A++ A Sbjct: 241 TVTAGNASGINDGAAILVVMSEKKAQELGLTPLAAIRSYASAGVDPAIMGTGPIPASRKA 300 Query: 300 IEKAGWTVDELDLIESNEAFAAQSLAVAKDLKFDMNKVNVNGGAIALGHPIGASGARILV 359 +EKAGWTVD+LDL+E+NEAFAAQ++AV K+L +D KVNVNGGAIA+GHPIGASGARIL Sbjct: 301 LEKAGWTVDDLDLVEANEAFAAQAIAVNKELGWDTAKVNVNGGAIAIGHPIGASGARILT 360 Query: 360 TLVHAMQKRDAKKGLATLCIGGGQGTAILLEK 391 TL++ M++RDAKKGLATLCIGGGQGTA+ +E+ Sbjct: 361 TLLYEMKRRDAKKGLATLCIGGGQGTALTVER 392 Lambda K H 0.315 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 568 Number of extensions: 20 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 392 Length of database: 392 Length adjustment: 31 Effective length of query: 361 Effective length of database: 361 Effective search space: 130321 Effective search space used: 130321 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate WP_073037060.1 BUB04_RS03720 (acetyl-CoA C-acetyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.30061.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-160 519.3 3.3 3.4e-160 519.1 3.3 1.0 1 lcl|NCBI__GCF_900129305.1:WP_073037060.1 BUB04_RS03720 acetyl-CoA C-acety Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900129305.1:WP_073037060.1 BUB04_RS03720 acetyl-CoA C-acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 519.1 3.3 3.4e-160 3.4e-160 1 385 [] 6 391 .. 6 391 .. 0.99 Alignments for each domain: == domain 1 score: 519.1 bits; conditional E-value: 3.4e-160 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaag 69 iv+avRtp+g++g+sl+ ++a+ L++++++e+l+r l+p+++devilGnvlqa++++n+aR++a+ ag lcl|NCBI__GCF_900129305.1:WP_073037060.1 6 IVSAVRTPVGSFGKSLAAIPAVALGVTAVREALKRIALSPDQVDEVILGNVLQAAQGQNPARQVAVHAG 74 8******************************************************************** PP TIGR01930 70 lpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakled 138 +p++vpa+t+n+vCaSgl++v laaq++ aG+a+vvvaGG+E+mS +p+ l+++ r++ ++g+ +l d lcl|NCBI__GCF_900129305.1:WP_073037060.1 75 IPHEVPAFTINKVCASGLKSVVLAAQAVLAGDAEVVVAGGIENMSAAPYALPKA--RWGQRMGHGELVD 141 ****************************************************98..8************ PP TIGR01930 139 qllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk... 201 ++ d+ ++++mg+tAen+a+++gisReeqD++al+S+qka++Ai+eg+fkdeivpve++++ lcl|NCBI__GCF_900129305.1:WP_073037060.1 142 LMILDGlwdIFNGYHMGITAENVAERFGISREEQDAFALASQQKAETAIKEGRFKDEIVPVEIPQRkgd 210 **9999***99*****************************************************999*9 PP TIGR01930 202 kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplariv 270 ++ +++De++r +ttle+LakLkpafk+ +g tvtAgN+s++nDGAa+l++mse++a+elgltpla i+ lcl|NCBI__GCF_900129305.1:WP_073037060.1 211 PVLFDTDEHPRFGTTLEALAKLKPAFKK-DG-TVTAGNASGINDGAAILVVMSEKKAQELGLTPLAAIR 277 9*************************95.9*.6************************************ PP TIGR01930 271 saavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAi 339 s+a agvdp++mg+gp+pA++kaL+kag++++d+dlvE nEAFAaq++av+kelg d++kvNvnGGAi lcl|NCBI__GCF_900129305.1:WP_073037060.1 278 SYASAGVDPAIMGTGPIPASRKALEKAGWTVDDLDLVEANEAFAAQAIAVNKELG-WDTAKVNVNGGAI 345 *******************************************************.88*********** PP TIGR01930 340 AlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 A+GHP+GasGari++tll+e+k+r++k GlatlC+ggGqG+A+ +e lcl|NCBI__GCF_900129305.1:WP_073037060.1 346 AIGHPIGASGARILTTLLYEMKRRDAKKGLATLCIGGGQGTALTVE 391 ******************************************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (392 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 8.85 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory