Alignments for a candidate for mcm-large in Desulfacinum infernum DSM 9756

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Desulfacinum infernum DSM 9756

Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_073040518.1 BUB04_RS13745 methylmalonyl-CoA mutase

Query= BRENDA::O74009
         (563 letters)



>NCBI__GCF_900129305.1:WP_073040518.1
          Length = 562

 Score =  613 bits (1581), Expect = e-180
 Identities = 307/563 (54%), Positives = 414/563 (73%), Gaps = 5/563 (0%)

Query: 4   FDKEVLKKIKEEEKRWEETTVKKFLEKAPERKEKFMTDDGFEIKRIYTPADLGEDWNYME 63
           F +  L++++ E + W     +K L++ PER E+F T    E+K +YTP D+ E  +Y E
Sbjct: 2   FSESQLERMEREYQDWLGE-YQKALQRLPERLERFSTVSDMEVKSLYTPLDIKEK-DYFE 59

Query: 64  KLGFPGEYPFTRGVYATMYRGRIWTMRQYAGYATAEESNKRYKYLLSQGQTGLSVAFDLP 123
            LGFPG+YPFTRGV  TMYR R+WTMR +AG  TAE++NKR+ YL+  G+TGLS AFD P
Sbjct: 60  DLGFPGQYPFTRGVQPTMYRARLWTMRMFAGLGTAEDTNKRFHYLIEHGETGLSTAFDFP 119

Query: 124 TQLGYDSDHPLAEGEVGKVGVAIDSLWDMRILFDGIPLDKVSTSMTINSTAANLLAMYIL 183
           T +GYD+D P+A GE GK GVAID++ DMR LF GI L++V+TSMTIN  A+ + AMYI 
Sbjct: 120 TLMGYDTDAPVARGECGKCGVAIDTIDDMRRLFAGINLEEVTTSMTINPPASVIWAMYIA 179

Query: 184 VAEEQGVSQEKLRGTVQNDILKEYIARGTYIFPPQPSMRLTTDIIMYCAENVPKWNPISI 243
            AE +G  ++KL GT+QND LKE+IA+ T + PP+PS+RL  D + +    VP+WN ISI
Sbjct: 180 NAENEGYDRKKLGGTIQNDCLKEFIAQKTLMLPPEPSLRLVVDTVEFGTYEVPRWNTISI 239

Query: 244 SGYHIREAGANAVQEVAFTLADGIEYVKAVIERGMDVDKFAPRLSFFFAAHNNFLEEIAK 303
           SGYHIREAGA AVQE+AFT+ DGI YV+  I+RG+ VD FA RLSFF+ +H +F EEIAK
Sbjct: 240 SGYHIREAGATAVQELAFTIYDGITYVEECIKRGLKVDDFAGRLSFFWNSHIDFFEEIAK 299

Query: 304 FRAARRLWAYIMKEWFNAKNPRSMMLRFHTQTAGSTLTAQQPENNIVRVAIQALAAVLGG 363
            RAARR+WA IMKE F A+ PRSM+ RFHTQTAG +LTAQ+P NNI+R   +ALAAVLGG
Sbjct: 300 MRAARRMWARIMKERFKAQKPRSMLCRFHTQTAGCSLTAQEPYNNIIRTTTEALAAVLGG 359

Query: 364 TQSLHTNSYDEALSLPTEKSVRIALRTQQIIAYESGVVDTVDPLGGAYYIEWLTDHIYEE 423
           TQSLHTNS DE   +P+E++VRIALRTQQI+A E+GV + +DPL G+Y++E LTD + EE
Sbjct: 360 TQSLHTNSLDEVYMIPSEEAVRIALRTQQILADETGVANVIDPLAGSYFVEALTDKMEEE 419

Query: 424 ALKYIEKIQKMGGMMRAIERGYVQKEIAEAAYKYQKEIEEGKRIIVGVNAFVTDEPIEVE 483
           A+ YI K+ ++GGM+ AIE+ Y Q EIA+AAY++Q+++E G++II+GVN F ++ P    
Sbjct: 420 AMAYIRKLDELGGMVAAIEKDYPQMEIADAAYQFQQQVERGEKIIIGVNKFPSERPELEF 479

Query: 484 ILKVDPSIREKQIERLKKLRSERDNKKVQEALDKLRNAAEKE---DENLMPYIIEAHRHL 540
           +L++D  +   QIER ++ + +RD  K+Q+A+D+LR   E     + N+MP +I+A R  
Sbjct: 480 VLRIDDELENFQIERTRQFKEKRDKVKLQKAMDELRRRCEGPPCWENNVMPALIDAVRAG 539

Query: 541 ATLQEVTDVLREIWGEYRAPLIF 563
           AT QE  D+ RE++G Y  P  F
Sbjct: 540 ATEQECCDLYREVFGTYTDPGTF 562


Lambda     K      H
   0.318    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 838
Number of extensions: 38
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 563
Length of database: 562
Length adjustment: 36
Effective length of query: 527
Effective length of database: 526
Effective search space:   277202
Effective search space used:   277202
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources