Align Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate WP_073041507.1 BUB04_RS16430 3-hydroxyacyl-CoA dehydrogenase
Query= SwissProt::P28793 (715 letters) >NCBI__GCF_900129305.1:WP_073041507.1 Length = 681 Score = 307 bits (786), Expect = 1e-87 Identities = 206/661 (31%), Positives = 335/661 (50%), Gaps = 36/661 (5%) Query: 30 NKFNRLTLNELRQAVDAIKADASVKGVIVSSGKDVFIVGADITE--FVENFKLPDAELIA 87 N F+ L L +A++ + A+ VKG++++ +F GAD+T+ FV F+ A Sbjct: 35 NVFSEAALQSLNEAINKVLAEGDVKGLMLTGKPYIFAAGADLTQVPFVTTFEQGYQIGKA 94 Query: 88 GNLEANKIFSDFEDLNVPTVAAINGIALGGGLEMCLAADFRVMADSAK-IGLPEVKLGIY 146 G+ ++ DL PTVAA NG+ALGGGLE+ L D+R +A S +G PE +G+ Sbjct: 95 GHTIMKRLM----DLPFPTVAAYNGVALGGGLEIGLYCDYRTVAKSVPAMGFPECFIGLI 150 Query: 147 PGFGGTVRLPRLIGVDNAVEWIASGKENR-----AEDALKVSAVDAVVTADKLGAAALDL 201 PG+GG +L+G + A+E I N+ A ++ D + + +L Sbjct: 151 PGWGGCTLATKLLGPEKALELIVFNALNQNRMINGPQAFEIGIADRLFDGAEFFDESLQF 210 Query: 202 IKRAISGELDYKAKRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPVEAIKTIQK 261 + ISG+ + + PK LK A AK FV + PAP AI+ I+ Sbjct: 211 LVNIISGK-EKVERPAPKTANLKA-------AVSKAKAFVDSKVHGAAPAPYRAIELIEG 262 Query: 262 AANFGRDKALEVEAAGFAKLAKTSASNCLIGLF-LNDQELKKKAKVYDKIAKDVKQAAVL 320 A + D+ E E L K+ C I F L ++ KK + D K VK+ ++ Sbjct: 263 AVKWDIDQGFEEENKALGDLVKSRQCKCSIYAFDLVNRYAKKVKGIPDVKPKPVKKVGII 322 Query: 321 GAGIMGGGIAYQSASK-GTPILMKDINEHGIEQGLAEAAKLLVGRVDKGRMTPAKMAEVL 379 GAG+M +A + G P++MKDI + +++G+A V+KGRM A+ + Sbjct: 323 GAGLMASQLAQVFIYRLGVPVVMKDIKQEFVDKGVAYVRGEFQKMVEKGRMPEARARYLS 382 Query: 380 NGIRPTLSYGDFGNVDLVVEAVVENPKVKQAVLAEVENHVREDAILASNTSTISISLLAK 439 + + TLSY DF + D V+EAV E +K+ V AEVE V + ILA+NTS++S++ + Sbjct: 383 SLVHGTLSYDDFADCDFVIEAVFERMDIKKQVFAEVEAVVSPECILATNTSSLSVTEMGS 442 Query: 440 ALKRPENFVGMHFFNPVHMMPLVEVIRGEKSSDLAVATTVAYAKKMGKNPIVVNDCPGFL 499 LK P+ +G HFFNPV ++PLVEVI+ ++ D +AT AKK+ K ++V D P FL Sbjct: 443 DLKHPQRVIGFHFFNPVAVLPLVEVIKTPQTDDQTLATAFDLAKKLKKTGVLVKDAPAFL 502 Query: 500 VNRVLFPYFGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYLMDVVGIDTGHHGRDVMAEG 559 VNR+L +V G DF+++D+ + G PM P L+ +VG +H + + Sbjct: 503 VNRLLTKMLSDCLTMVDEGADFIQVDEALLALGMPMAPFDLLALVGFPVAYHVCETLNRA 562 Query: 560 FPDRMKDDRRSAIDALYEAKRLGQKNGKGFYAYEADKKGKQKKLVDSSVLEVLKPIVYEQ 619 F R ++A + K+L Y G + K V+ V ++ + Sbjct: 563 F-----GPERFPLNANF--KKLIDAGINSIYV-----PGSKVKKVNPEVEKLWEK--KGD 608 Query: 620 RDVTDEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVYGIGFPLFRGGALRYIDSIG 679 ++ ++I + ++ L E L++ +V+++ + D+ ++ G G+P F GG Y+D G Sbjct: 609 KEFHPDEIRDRVLSNLAKEIDLILKEKVVDSSRDVDLAMIMGAGWPFFMGGITMYLDMAG 668 Query: 680 V 680 + Sbjct: 669 I 669 Lambda K H 0.318 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1140 Number of extensions: 59 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 715 Length of database: 681 Length adjustment: 39 Effective length of query: 676 Effective length of database: 642 Effective search space: 433992 Effective search space used: 433992 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory