Alignments for a candidate for mcm-large in Desulfacinum hydrothermale DSM 13146

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Desulfacinum hydrothermale DSM 13146

Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_084058822.1 B9A12_RS14285 methylmalonyl-CoA mutase

Query= BRENDA::O74009
         (563 letters)



>NCBI__GCF_900176285.1:WP_084058822.1
          Length = 562

 Score =  611 bits (1575), Expect = e-179
 Identities = 308/564 (54%), Positives = 415/564 (73%), Gaps = 7/564 (1%)

Query: 4   FDKEVLKKIKEEEKRWEETTVKKFLEKAPERKEKFMTDDGFEIKRIYTPADL-GEDWNYM 62
           F +  L++++ E + W     +K L++ PER E+F T    E+K +YTP D+ G+D  Y 
Sbjct: 2   FSESQLERMEREYQDWLGE-YQKALQRLPERLERFSTVSDMEVKSLYTPLDIKGKD--YF 58

Query: 63  EKLGFPGEYPFTRGVYATMYRGRIWTMRQYAGYATAEESNKRYKYLLSQGQTGLSVAFDL 122
           + LGFPG+YPFTRGV  TMYR R+WTMR +AG  TAE++NKR+ YL+  G+TGLS AFD 
Sbjct: 59  QDLGFPGQYPFTRGVQPTMYRARLWTMRMFAGLGTAEDTNKRFHYLIEHGETGLSTAFDF 118

Query: 123 PTQLGYDSDHPLAEGEVGKVGVAIDSLWDMRILFDGIPLDKVSTSMTINSTAANLLAMYI 182
           PT +GYD++ P+A GE GK GVAID++ DM+ LF GI L++V+TSMTIN  A+ + AMYI
Sbjct: 119 PTLMGYDTEAPVARGECGKCGVAIDTIEDMQRLFAGINLEEVTTSMTINPPASVIWAMYI 178

Query: 183 LVAEEQGVSQEKLRGTVQNDILKEYIARGTYIFPPQPSMRLTTDIIMYCAENVPKWNPIS 242
             AE +G  ++KL GT+QND LKE+IA+ T + PP+PS+RL  D + +    VP+WN IS
Sbjct: 179 ANAENEGYDRKKLGGTIQNDCLKEFIAQKTLMLPPEPSLRLVVDTVEFGTYEVPRWNTIS 238

Query: 243 ISGYHIREAGANAVQEVAFTLADGIEYVKAVIERGMDVDKFAPRLSFFFAAHNNFLEEIA 302
           ISGYHIREAGA AVQE+AFT+ DGI YV+  I+RG+ VD FA RLSFF+ +H +F EEIA
Sbjct: 239 ISGYHIREAGATAVQELAFTIYDGITYVEECIKRGLKVDDFAGRLSFFWNSHIDFFEEIA 298

Query: 303 KFRAARRLWAYIMKEWFNAKNPRSMMLRFHTQTAGSTLTAQQPENNIVRVAIQALAAVLG 362
           K RAARRLWA IMKE F A+ PRSMM RFHTQTAG +LTAQ+P NNI+R   +ALAAVLG
Sbjct: 299 KMRAARRLWARIMKERFKAQKPRSMMCRFHTQTAGCSLTAQEPYNNIIRTTTEALAAVLG 358

Query: 363 GTQSLHTNSYDEALSLPTEKSVRIALRTQQIIAYESGVVDTVDPLGGAYYIEWLTDHIYE 422
           GTQSLHTNS DE   +P+E++VRIALRTQQI+A E+GV + +DPL G+Y++E LTD + E
Sbjct: 359 GTQSLHTNSLDEVYMIPSEEAVRIALRTQQILADETGVANVIDPLAGSYFVEALTDKMEE 418

Query: 423 EALKYIEKIQKMGGMMRAIERGYVQKEIAEAAYKYQKEIEEGKRIIVGVNAFVTDEPIEV 482
           EA+ YI K+ ++GGM+ AIE+ Y Q EIA+AAY++Q+++E G++II+GVN F +D P   
Sbjct: 419 EAMAYIRKLDELGGMVAAIEKDYPQMEIADAAYQFQQQVERGEKIIIGVNKFPSDRPELE 478

Query: 483 EILKVDPSIREKQIERLKKLRSERDNKKVQEALDKLRNAAEKE---DENLMPYIIEAHRH 539
            +L++D  +   QIER ++ + +RD  K+Q+A+D+LR   E     + N+MP +I+A R 
Sbjct: 479 FVLRIDDELENFQIERTRQFKEKRDKVKLQKAMDELRRRCEGPPCWENNVMPALIDAVRA 538

Query: 540 LATLQEVTDVLREIWGEYRAPLIF 563
            AT QE  D+ RE++G Y  P  F
Sbjct: 539 GATEQECCDLYREVFGIYTDPGTF 562


Lambda     K      H
   0.318    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 823
Number of extensions: 37
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 563
Length of database: 562
Length adjustment: 36
Effective length of query: 527
Effective length of database: 526
Effective search space:   277202
Effective search space used:   277202
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources