Protein WP_084217447.1 in Xenophilus azovorans DSM 13620
Annotation: NCBI__GCF_000745855.1:WP_084217447.1
Length: 380 amino acids
Source: GCF_000745855.1 in NCBI
Candidate for 35 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-asparagine catabolism | aapM | hi | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 42% | 93% | 291.6 | ABC transporter for D-Alanine, permease component 1 | 43% | 272.3 |
| L-aspartate catabolism | aapM | hi | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 42% | 93% | 291.6 | ABC transporter for D-Alanine, permease component 1 | 43% | 272.3 |
| L-glutamate catabolism | aapM | hi | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 42% | 93% | 291.6 | ABC transporter for D-Alanine, permease component 1 | 43% | 272.3 |
| L-histidine catabolism | aapM | hi | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 42% | 93% | 291.6 | ABC transporter for D-Alanine, permease component 1 | 43% | 272.3 |
| L-leucine catabolism | aapM | hi | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 42% | 93% | 291.6 | ABC transporter for D-Alanine, permease component 1 | 43% | 272.3 |
| L-proline catabolism | aapM | hi | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 42% | 93% | 291.6 | ABC transporter for D-Alanine, permease component 1 | 43% | 272.3 |
| D-alanine catabolism | Pf6N2E2_5404 | med | ABC transporter for D-Alanine, permease component 1 (characterized) | 43% | 96% | 272.3 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | natH | lo | Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (characterized, see rationale) | 37% | 98% | 228.4 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | natH | lo | Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (characterized, see rationale) | 37% | 98% | 228.4 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | bztC | lo | BztC, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 39% | 80% | 204.5 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | bztC | lo | BztC, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 39% | 80% | 204.5 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-glutamate catabolism | bztC | lo | BztC, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 39% | 80% | 204.5 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| D-glucosamine (chitosamine) catabolism | AO353_21715 | lo | ABC transporter for D-glucosamine, permease component 1 (characterized) | 35% | 90% | 119 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | aatM | lo | ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 1 (characterized) | 35% | 96% | 115.9 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | aatM | lo | ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 1 (characterized) | 35% | 96% | 115.9 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-glutamate catabolism | gltK | lo | ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 1 (characterized) | 35% | 96% | 115.9 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-arginine catabolism | artM | lo | ABC transporter for L-Arginine, permease component 2 (characterized) | 36% | 89% | 114.8 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-glutamate catabolism | gltJ | lo | Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized) | 34% | 87% | 112.5 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | aatQ | lo | PP1070, component of Acidic amino acid uptake porter, AatJMQP (characterized) | 33% | 85% | 111.7 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | aatQ | lo | PP1070, component of Acidic amino acid uptake porter, AatJMQP (characterized) | 33% | 85% | 111.7 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| D-glucosamine (chitosamine) catabolism | AO353_21720 | lo | ABC transporter for D-glucosamine, permease component 2 (characterized) | 32% | 94% | 109.8 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-citrulline catabolism | AO353_03045 | lo | ABC transporter for L-Arginine and L-Citrulline, permease component 1 (characterized) | 34% | 89% | 109 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | peb1D | lo | Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) | 32% | 91% | 107.1 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | peb1D | lo | Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) | 32% | 91% | 107.1 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-lysine catabolism | hisQ | lo | ABC transporter for L-Lysine, permease component 1 (characterized) | 34% | 94% | 101.3 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-arginine catabolism | artQ | lo | Histidine transport system permease protein HisQ (characterized) | 31% | 95% | 94 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-histidine catabolism | hisQ | lo | Histidine transport system permease protein HisQ (characterized) | 31% | 95% | 94 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | bgtB' | lo | ABC-type permease for basic amino acids and glutamine (characterized, see rationale) | 33% | 60% | 90.5 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | bgtB' | lo | ABC-type permease for basic amino acids and glutamine (characterized, see rationale) | 33% | 60% | 90.5 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-glutamate catabolism | gluD | lo | GluD aka CGL1953, component of Glutamate porter (characterized) | 30% | 73% | 87.8 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-histidine catabolism | BPHYT_RS24005 | lo | Polar amino acid ABC transporter, inner membrane subunit; Flags: Precursor (characterized, see rationale) | 31% | 85% | 86.3 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-asparagine catabolism | bztB | lo | glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) | 31% | 54% | 83.6 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-aspartate catabolism | bztB | lo | glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) | 31% | 54% | 83.6 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-glutamate catabolism | bztB | lo | glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) | 31% | 54% | 83.6 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
| L-glutamate catabolism | gluC | lo | GluC aka CGL1952, component of Glutamate porter (characterized) | 32% | 76% | 77.4 | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) | 42% | 291.6 |
Sequence Analysis Tools
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MPCHRDGGVPAAVAADLGRDGHLQRARGAAGVAVSATPWRTELFGSPLRALVSLALAALL
GWALWHALDWALLRAVFQPDAQACRASAHGACWGVVAEKWRPLLFGRYPYAEQWRPALAV
TLLSAATLASAWPSCWRWWLAPLWAAALAACIALMRGGVAGLSVVPTSQWGGLPLTIALA
VVGLALAFPLALVLALARRSTWPAARALAAGYVELVRGVPLITVLFMASYLVPLLWPTGH
RPDVLLRVLAGLALFVAAYLAEIIRGGLQAVPRGQVEAATALGFGRWAVQRDIVLPQALR
MVVPALTNSVVGTLKDTSLVTVVGLFELTGALGLALGGDPVWRPFYLEGYLFIALIYWCL
CFGLSRYSRWLERRLRADAG
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory