Align actP-like component of L-lactate and L-malate uptake system (characterized)
to candidate WP_086508553.1 BZY95_RS03245 cation acetate symporter
Query= reanno::PV4:5209923 (572 letters) >NCBI__GCF_002151265.1:WP_086508553.1 Length = 598 Score = 654 bits (1687), Expect = 0.0 Identities = 338/599 (56%), Positives = 422/599 (70%), Gaps = 38/599 (6%) Query: 6 LTYLIVGFTFALYIGIAIWSRAGSTKEFYVAGGGVHPVMNGMATAADWMSAASFISLAGI 65 + L VG +FALYIGIAIW+RAGSTK+FYVAGGGVHPV NGMATAADWMSAASFIS+AG+ Sbjct: 6 INLLFVGGSFALYIGIAIWARAGSTKDFYVAGGGVHPVTNGMATAADWMSAASFISMAGL 65 Query: 66 VSFVGYDGSVYLMGWTGGYVLLALCMAPYLRKFGKFTVPDFIGDRYYSQAARTVAVVCAI 125 ++ GY S +LMGWTGGYV+LA+ +APYLRKFGKFTVPDFIGDR+YS+ AR VAV+C I Sbjct: 66 LASGGYANSTFLMGWTGGYVILAMLLAPYLRKFGKFTVPDFIGDRFYSKTARVVAVICLI 125 Query: 126 FICFTYIAGQMRGVGVVFSRFLEVEVDTGVYIGMAVVFFYAVLGGMKGITYTQVAQYCVL 185 TY+ GQM G GV FSRFLEV TG++I +VF YAV GGMKGITYTQVAQY VL Sbjct: 126 VASVTYVIGQMTGAGVAFSRFLEVPSSTGIWIAAGIVFLYAVFGGMKGITYTQVAQYIVL 185 Query: 186 IFAFMVPAIFISVMMTGHILPQLGFGAELVDAAGNNTGVYLLEKLDGLSAQLGFSQYTEG 245 I A+ +PA+FI++ +TG+ +P G + + +GV LL KLD + LGF YT Sbjct: 186 IIAYTIPAVFIAMQLTGNPIPMFG-----MFSTHTESGVPLLAKLDEVVTALGFRDYTAD 240 Query: 246 SKGMIDVFFITGALMFGTAGLPHVIVRFFTVPKVKDARVSAGWALVFIAIMYTTIPALAA 305 +++ T +LM GTAGLPHVI+RFFTVPKV DAR SAGWALVFIA++Y T PA+ + Sbjct: 241 VDNKLNMVLFTLSLMIGTAGLPHVIIRFFTVPKVADARWSAGWALVFIALLYLTAPAVGS 300 Query: 306 FSRVNMIETINGPESTG------------VAYETAPDWIKNWEKTGLIKWDDKNNDGKIY 353 +R+N+ TI PE G + YE PDWI+ WE TGLI + D+NNDG+I Sbjct: 301 MARLNLATTI-FPEMAGQVEDYQEALANPILYEERPDWIRTWEDTGLITFTDRNNDGRIQ 359 Query: 354 YAKG--------------ETNEMKIDRDIMVLATPEIANLPAWVIALVAAGGLAAALSTS 399 E NE+ ++ DI+VLA PEIANLP WVI L+AAGG+AAALST+ Sbjct: 360 MYNDANPGFQPIASELGWEGNELTVNNDILVLANPEIANLPGWVIGLIAAGGIAAALSTA 419 Query: 400 AGLLLVISTSVSHDLLKKNFMPDISDKQELLYARIAAALGIVMAGYFGINPPGFVAAVVA 459 AGLLL IS+++SHDL+K P IS+K E+L ARI+ A I++A Y G+NPPGF A VVA Sbjct: 420 AGLLLAISSAISHDLIKNTINPSISEKGEMLAARISMAGAILLATYLGLNPPGFAAQVVA 479 Query: 460 IAFGLAASSLFPAIIMGIFSRTMNKEGAIAGMVIGLLFSASYIIYFK--FVNPGDNN--- 514 +AFG+AA+SLFP ++MGIFS MN +GAI GM+ GL+ + YI + F PG N Sbjct: 480 LAFGIAAASLFPVLMMGIFSTRMNSKGAICGMLAGLISTLLYIFTYLGWFFIPGTNMLAN 539 Query: 515 -ASNWLFGISPEGIGMLGMIINFAVAFIVSKVTAAVPQNVVDMVESIRFPKGAGEAHDH 572 +NWL GISP G +G +INFAVA+ VS T PQ + ++VES+R+PKGAG A H Sbjct: 540 VEANWLLGISPLSFGAVGAMINFAVAYAVSSATEEPPQEIQELVESVRYPKGAGAATGH 598 Lambda K H 0.326 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1122 Number of extensions: 41 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 572 Length of database: 598 Length adjustment: 37 Effective length of query: 535 Effective length of database: 561 Effective search space: 300135 Effective search space used: 300135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory