Align Glycine betaine/proline/choline/ectoine transporter VP1456 (characterized)
to candidate WP_086509110.1 BZY95_RS06250 BCCT family transporter
Query= SwissProt::Q87PP5 (562 letters) >NCBI__GCF_002151265.1:WP_086509110.1 Length = 524 Score = 613 bits (1582), Expect = e-180 Identities = 304/513 (59%), Positives = 386/513 (75%), Gaps = 5/513 (0%) Query: 47 DTTDYQVGQDNVQKWGFDIHNPVFGISAGLVVFCLISLLLVEPVTARDALNGIKNGIIEQ 106 D + GQDN Q G D H+PVF +SA L + I L+ P A L + IE Sbjct: 5 DAPILEPGQDNAQVLGMDFHHPVFPLSA-LAILAFILYALIYPDAANSHLTAARGFSIEH 63 Query: 107 FDAFFMWSTNFFLLFAVGLLFSPLGKIRLGGKEATPDHSTVSWLSMLFAAGMGIGLLFWS 166 FD FM + N F+L + L+ PLG+IRLGG +A P++ST SW +MLFAAGMGIGL+FWS Sbjct: 64 FDWLFMIAGNLFVLLCLTLIVLPLGRIRLGGSQARPEYSTTSWFAMLFAAGMGIGLMFWS 123 Query: 167 VAEPTAYFTDWWGTPLNAEAYSADAKSLAMGATMFHWGVHGWSIYALVALALAFFAFNKG 226 VAEP AY+TDW+GTPLNAEA++ S A+GATMFHWG+H W+IY +V L+LAFFA+N+G Sbjct: 124 VAEPVAYYTDWYGTPLNAEAHTPAGASAAIGATMFHWGLHPWAIYGVVGLSLAFFAYNRG 183 Query: 227 LPLSLRAAFYPIFGDRAWGWLGHVIDILAVLSTLFGLATSLGLGAQQATSGINHVFGLNG 286 LPL+LR+AF PI G+R GW GH+IDI+AVL+T+FGLATSLG GA QA G+N+++G+ Sbjct: 184 LPLTLRSAFTPILGERVRGWFGHIIDIVAVLATIFGLATSLGFGASQAAGGLNYLWGVPN 243 Query: 287 GIGTQMVVIAFVTFIAVLSVVRGIDGGVKLLSNVNMIVAFALLIFITFITFDTAM--GSL 344 IGTQ+ +I VT +A+ SV RGIDGGVKL S +NM++A LL F+ +T + +L Sbjct: 244 TIGTQLAIIVVVTAVALFSVWRGIDGGVKLFSKINMMLALGLLTFV-LVTGGVLLFVNNL 302 Query: 345 VDTTMAYIQNIIPLSNPHGREDETWMHGWTVFYWAWWVSWSPFVGMFIARVSKGRTVREF 404 TT+AY+ +I+PLSN GR+D TW HGWTVFYWAWW+SWSPFVGMFIARVS+GRTVREF Sbjct: 303 WHTTLAYVSHIVPLSNWVGRDDTTWYHGWTVFYWAWWISWSPFVGMFIARVSRGRTVREF 362 Query: 405 LFAVIVIPTLVTLVWMSVFGGIALDQVVNKVGELGANGLTDISLTLFHVYDVLPYSSVIS 464 L AV+++PTLVTLVWMS FGG AL+Q VG L A+G++D+SL +F + + LP +++ S Sbjct: 363 LIAVLLVPTLVTLVWMSAFGGTALNQSAAGVGAL-ADGISDVSLAMFQMLEHLPLTTLTS 421 Query: 465 ILSIVLILVFFITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVMLWVGGKEA 524 L+I+L+L+FFITSSDSGSLVID+ITAGGK DAP QR+FWA +EG IA ++L+ GG A Sbjct: 422 SLAILLVLIFFITSSDSGSLVIDNITAGGKTDAPRGQRVFWAVLEGVIAGILLYGGGSTA 481 Query: 525 LQALQSGVVATGLPFTFVLLLMCVSLVKGLRTE 557 L ALQ+G VATGLPFT VLL M LVKGLR E Sbjct: 482 LGALQAGAVATGLPFTLVLLGMAYCLVKGLREE 514 Lambda K H 0.327 0.141 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1026 Number of extensions: 65 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 562 Length of database: 524 Length adjustment: 35 Effective length of query: 527 Effective length of database: 489 Effective search space: 257703 Effective search space used: 257703 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory