Alignments for a candidate for acs in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Halomonas desiderata SP1

Align Acyl-CoA synthetase short-chain family member 3, mitochondrial; Acetate--CoA ligase 3; Acyl-CoA synthetase short-chain family member 3; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 (characterized)
to candidate WP_086509797.1 BZY95_RS10010 propionyl-CoA synthetase

Query= SwissProt::Q9H6R3
         (686 letters)



>NCBI__GCF_002151265.1:WP_086509797.1
          Length = 636

 Score =  638 bits (1645), Expect = 0.0
 Identities = 309/626 (49%), Positives = 420/626 (67%), Gaps = 9/626 (1%)

Query: 62  YKTHFAASVTDPERFWGKAAEQISWYKPWTKTLENKHSPSTRWFVEGMLNICYNAVDRHI 121
           YK  F  S+  P+ FW   A +I W+ P    LE       RW+ +G LN+C+ A+D H+
Sbjct: 4   YKNEFQRSIEQPQSFWADQARRIPWFTPPKTILEYDEQGHARWYTDGELNLCHAALDHHV 63

Query: 122 ENGKGDKIAIIYDSPVTNTKATFTYKEVLEQVSKLAGVLVKHGIKKGDTVVIYMPMIPQA 181
           + G+GD+ AI +DSPVT  K T TY+E+ ++V+  AG L   G++KGD VVIYMPM+P+A
Sbjct: 64  DQGRGDQPAIYWDSPVTGGKRTLTYREMRDEVALFAGALEGLGVEKGDRVVIYMPMVPEA 123

Query: 182 MYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKVVVTASFGIEPGRRVEYVPLVEEA 241
           +  M ACAR+GA+HS++FGGFA  EL+ RID  KPKVVV AS G+E  R + Y P +  A
Sbjct: 124 LVAMYACARLGAVHSVVFGGFAPHELAVRIDDAKPKVVVAASCGVEIDRVIAYQPNIAAA 183

Query: 242 LKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSG 301
           +++ +HKPD   IY +   +   L P RDLDW E +  A+  DCVPV    PLY+LYTSG
Sbjct: 184 IELSEHKPDAC-IYLQRQQQLAELGP-RDLDWSELLESAEPADCVPVKGSDPLYVLYTSG 241

Query: 302 TTGLPKGVIRPTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNT 361
           TTG PKGV+R T GYAV LH+SM +IY + PG+V+++ASD+GWVVGHSYI Y PLL G T
Sbjct: 242 TTGKPKGVVRDTAGYAVALHYSMETIYDVAPGDVFFSASDVGWVVGHSYIVYAPLLRGCT 301

Query: 362 TVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTL 421
           TV+YEGKPV TPDAGA++R+++++ V + FTAPTA RAI+++DP   L +QY ++  K L
Sbjct: 302 TVVYEGKPVKTPDAGAFWRLISQYRVKSFFTAPTAFRAIKKEDPDGILLQQYDISCLKAL 361

Query: 422 FVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKS--- 478
           ++AGER D  T  W  ++  VPV+DHWWQTETG PI A+  GL     P P +AG +   
Sbjct: 362 YLAGERLDPPTFHWLDDLLDVPVIDHWWQTETGWPIAANLQGL----EPMPTKAGSATVP 417

Query: 479 VPGYNVMILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTM 538
           VPG+NV IL+   ++      G++V+K P+PPG   G+W + + F   Y   FPGYY T 
Sbjct: 418 VPGFNVQILNREGEQAAPMEQGSVVIKQPMPPGCLIGVWGDPQRFHSAYMAAFPGYYLTG 477

Query: 539 DAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVP 598
           D GY DE+GYL++M R DDVINVAGHR+S G +EE + +H  VA+CAV+G  D LKG +P
Sbjct: 478 DGGYFDEQGYLFIMGRTDDVINVAGHRLSTGEMEEVVGAHRAVAECAVIGIHDALKGQIP 537

Query: 599 LALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAI 658
           + L + +   +  E  +  E++  VR+ IGP+A F+  + V +LPKTRSGKI R  L  I
Sbjct: 538 VGLVIPKDGFDGDEVALENELIALVREKIGPIACFKQVLVVNRLPKTRSGKILRKLLRNI 597

Query: 659 VNGKPYKITSTIEDPSIFGHVEEMLK 684
            +GK Y + STI+DP+    V E +K
Sbjct: 598 ADGKEYGVPSTIDDPASLQDVHEAMK 623


Lambda     K      H
   0.318    0.136    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1205
Number of extensions: 52
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 686
Length of database: 636
Length adjustment: 38
Effective length of query: 648
Effective length of database: 598
Effective search space:   387504
Effective search space used:   387504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources