Align Propionyl-CoA carboxylase alpha chain; EC 6.4.1.3 (characterized)
to candidate WP_086510401.1 BZY95_RS13285 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha
Query= SwissProt::Q5LUF3 (681 letters) >NCBI__GCF_002151265.1:WP_086510401.1 Length = 681 Score = 501 bits (1291), Expect = e-146 Identities = 296/697 (42%), Positives = 408/697 (58%), Gaps = 48/697 (6%) Query: 2 FNKILIANRGEIACRVIKTARKMGISTVAIYSDADKQALHVQMADEAVHIGPPPANQSYI 61 F+ +L+ANRGEIACRV++TAR MG+ TVA+YSDAD A HV+ ADEAV +GP A +SY+ Sbjct: 11 FDTLLVANRGEIACRVMRTARAMGLRTVAVYSDADANARHVREADEAVRLGPAAARESYL 70 Query: 62 VIDKVMAAIRATGAQAVHPGYGFLSENSKFAEALEAEGVIFVGPPKGAIEAMGDKITSKK 121 ++ V+ A + TGA A+HPGYGFLSEN F +AL+A G+ FVGPP AI AMGDK +K Sbjct: 71 KVEAVVEAAKRTGAGAIHPGYGFLSENGAFVQALDAAGITFVGPPASAIAAMGDKSAAKA 130 Query: 122 IAQEANVSTVPGYMGLIEDADEAVKIS--NQIGYPVMIKASAGGGGKGMRIAWNDQEARE 179 A V VPGY G +D D+A+ S ++IGYPV++KASAGGGGKGMR+ ++ + Sbjct: 131 RMANAGVPLVPGYHG--DDQDDALLKSEADKIGYPVLLKASAGGGGKGMRVVEAPEQFQA 188 Query: 180 GFQSSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLCDSHGNGIYLGERECSIQRRNQKVV 239 + E+ +FGD R+ IEK++TQPRH+E+QV CDSHGNG+YL ER+CS+QRR+QKV+ Sbjct: 189 ALDGCRRESQAAFGDTRMLIEKYLTQPRHVEVQVFCDSHGNGVYLFERDCSVQRRHQKVL 248 Query: 240 EEAPSPFLDEATRRAMGEQAVALAKAVGYASAGTVEFIVDGQKNFYFLEMNTRLQVEHPV 299 EEAP+P + RR MGE AV AK +GY AGTVEF++D +FYF+EMNTRLQVEHPV Sbjct: 249 EEAPAPGMTAELRREMGEAAVRAAKEIGYVGAGTVEFLLDKDGSFYFMEMNTRLQVEHPV 308 Query: 300 TELITGVDLVEQMIRVAAGEPLSITQGDVKLTGWAIENRLYAEDPYRGFLPSIGRLTRYR 359 TE+ITG DLVE +RVA GE L + Q ++ +TG + E RLYAEDP + FLP+ GRL R+ Sbjct: 309 TEMITGQDLVEWQLRVAMGETLPLQQEELTITGHSFEARLYAEDPEQDFLPATGRLERF- 367 Query: 360 PPAETAAGPLLVNGKWQGDAPSGEAAVRNDTGVYEGGEISMYYDPMIAKLCTWAPTRAAA 419 ++ K G P VR D+GV G +SM+YDPM+AKL R A Sbjct: 368 ----------AMDLKGAGLDP---RQVRLDSGVETGDVVSMHYDPMLAKLIVHGDDRDQA 414 Query: 420 IEAMRIALDSFEVEGIGHNLPFLSAVMDHPKFISGDMTTAFIAEEYPEGFEGVNLPETDL 479 + + AL + +V+G+ N FL + HP F + ++ T FI + F P+ Sbjct: 415 LSTLNRALAALDVQGVVTNRAFLQRLATHPAFRNAELDTRFIEKNEATLF----APKQYS 470 Query: 480 RRVAAAAAAMHRVAEIRRTRVSGRMDNHER-RVGTEWVVTLQGADFPVTIAADHDGSTVS 538 A AA + R D H+ R+ + + D +IA HD V Sbjct: 471 IEEYAGAALIGLNQLARECESDSPWDRHDGFRLNAPHTIRISLCD--PSIAQVHDAEGVV 528 Query: 539 FDDGSSMRVTSDWTPGDQLANLMVDGAPLVLKVGKISG--------GFRIRTRG-ADLKV 589 +G+ W +L + G L ++ + G G R R + D V Sbjct: 529 VVEGTRASGADPW-------DLTIGGETLTARLQHLEGDAVAVTLNGHRRRLQARRDGNV 581 Query: 590 HVRTPRQAELARLMPEKLPP------DTSKMLLCPMPGLIVKVDVEVGQEVQEGQALCTI 643 V + E RL ++ + L PM G +V + VE GQ+V++G L + Sbjct: 582 VVMVDPRGE-TRLFWRRIDAIDHGHHEAESTLTAPMHGTVVALLVEAGQKVEKGMPLMVM 640 Query: 644 EAMKMENILRAEKKGVVAKINASAGNSLAVDDVIMEF 680 EAMKME+ + A G V + +AG+++ DV++EF Sbjct: 641 EAMKMEHTMTAPADGHVESFHFNAGDTVGQGDVLLEF 677 Lambda K H 0.317 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1139 Number of extensions: 64 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 681 Length of database: 681 Length adjustment: 39 Effective length of query: 642 Effective length of database: 642 Effective search space: 412164 Effective search space used: 412164 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory