Alignments for a candidate for pimF in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Halomonas desiderata SP1

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_086510728.1 BZY95_RS15080 fatty acid oxidation complex subunit alpha FadB

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_002151265.1:WP_086510728.1
          Length = 725

 Score =  348 bits (893), Expect = e-100
 Identities = 240/709 (33%), Positives = 363/709 (51%), Gaps = 30/709 (4%)

Query: 6   TRATQDQVAIVTVD--SPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGA 63
           T+   D VA++T D     +N LS+AV   + E V A  A+  ++ +V+  A  +FI GA
Sbjct: 14  TKNGGDDVAMLTFDLKDESINKLSSAVIAELDEAVQAIRAESGLKGLVIGSAKESFIVGA 73

Query: 64  DITEF----GKPPQ-----PPALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRV 114
           DITEF    GK  +        ++D+  A+E+ P PT+ AI+G ALGGG EV L   FRV
Sbjct: 74  DITEFQTMFGKGAEFLESMLMKVHDIFNAIEDLPFPTVTAINGLALGGGCEVTLTTDFRV 133

Query: 115 AVKEAKLGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVV 174
              +AK+GLPE KLG+LPG GG  RLPR +G + AV+ I GG+   A  ALK G V+ VV
Sbjct: 134 MGDKAKIGLPETKLGILPGWGGCVRLPRMIGSDNAVEWIAGGTENRADAALKVGAVDAVV 193

Query: 175 EN--LVAGAVAFAKKVLAEKRPLRRLRDDDS---KLAAAKADRSIFTNAVAAMTKKARGL 229
            N  L A A+    +  A +   +  R + +   KL A +   +  T       K     
Sbjct: 194 PNDQLEAAALDILARANAGELDYQARRAEKTGPLKLNAIEQMMAFETAKGYVAGKAGPHY 253

Query: 230 EAPFACADAIGAAIDLPFEEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDG 289
            AP     AI              E + F KL ++D +      F  ++   K  G  + 
Sbjct: 254 PAPIEAIKAIQKGAGEERARAQAIEAKAFAKLAMTDVAFNLVGLFMNDQVVKKKAGKFE- 312

Query: 290 TKPRPVSRVAIIGAGTMGGGIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARG 349
            + + V++ A++GAG MGGGIA   A+ G P+ + +  EE ++ GL   +K +     RG
Sbjct: 313 KQAKAVNQTAVLGAGIMGGGIAYQSASKGTPILMKDIKEEAVELGLKESRKLFAKQVERG 372

Query: 350 GLPPDAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASN 409
            L  +  A+++ LI   +   +    DL++EAV E   VK  V T V+       +L SN
Sbjct: 373 KLTTEQMAEKLTLIRPTLSYGDFGHVDLVVEAVVENPKVKDAVLTEVEGLVSEDTILTSN 432

Query: 410 TSYLSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKV 469
           TS +SI  +A   KRP++  GMHFF+P + M L E++RG KT   A+   V+ A+ + K 
Sbjct: 433 TSTISITRLAQNLKRPENFCGMHFFNPVHRMPLVEVIRGEKTGDAAVAATVAYARAMGKT 492

Query: 470 PVVVGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDI 529
           P+VV  C GF+ NR+L         L+ +GA  Q+VD V+ KFG PMGP  + D+ G+D 
Sbjct: 493 PIVVNDCPGFLVNRVLFPYFGGFSLLVEQGADFQRVDKVMEKFGWPMGPAYLLDVVGMDT 552

Query: 530 ----------GWRSRKDRGIKSEIADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVET 579
                     G+  R  R  K+ I   + E  R GQK  KG+Y YE+  +   P    + 
Sbjct: 553 AVHANEVMAEGFPERMARDGKTAI-QVMYENERLGQKNDKGFYAYEEDKKG-KPKKVSDD 610

Query: 580 LINDTLAKLGLKRRDITDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPI 639
              + + ++ +  ++ +DEEI+ RM+ P+  E  R LE+ I   P++ D+  +YG G+P 
Sbjct: 611 KAYELVKQVVVSEKEFSDEEIIARMMVPLCLETVRCLEDDIVETPAEADMALIYGIGFPP 670

Query: 640 YRGGPMHYADSVGLKHIAERLSAYAKATNDPSLEPAPLLARLAAEGKTF 688
           +RGG + Y D++G+    +     A+    P   P   L ++A  G+ F
Sbjct: 671 FRGGALRYIDAMGVAEFVKLADGLAEELG-PLYAPTDKLRKMAESGERF 718


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1060
Number of extensions: 51
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 725
Length adjustment: 39
Effective length of query: 660
Effective length of database: 686
Effective search space:   452760
Effective search space used:   452760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources