Align Acetate/butyrate--CoA ligase AAE7, peroxisomal; AMP-binding protein 7; AtAMPBP7; Acetyl-CoA synthetase; Acyl-activating enzyme 7; Butyryl-CoA synthetase; Protein ACETATE NON-UTILIZING 1; EC 6.2.1.1; EC 6.2.1.2 (characterized)
to candidate WP_086511178.1 BZY95_RS17500 acyl-CoA synthetase
Query= SwissProt::Q8VZF1 (569 letters) >NCBI__GCF_002151265.1:WP_086511178.1 Length = 543 Score = 614 bits (1583), Expect = e-180 Identities = 300/549 (54%), Positives = 386/549 (70%), Gaps = 16/549 (2%) Query: 11 DLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRSIG 70 DLPK AN+ AL+PL F++R+A ++P +VIHG +WR+T+ RCRRLASAL R I Sbjct: 9 DLPKTQANHVALSPLTFIERSASIYPDYPAVIHGDIRRSWRETWARCRRLASALEKRGIK 68 Query: 71 PGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQEFF 130 PG TVA + PN+PAM+EAHFGVP+ G VLN +NIRL+A +A++L H ++ ++VD EF Sbjct: 69 PGETVAAMLPNVPAMFEAHFGVPLAGCVLNTLNIRLDAEAIAYMLEHGEAQAVLVDPEFA 128 Query: 131 TLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNYPW 190 + ED++ + K PL+I + D E+ + G +EYE LA GDP Y + Sbjct: 129 GVIEDAVSRLAIK-------PLVIDVDDALYEGETRHI----GELEYEALLAEGDPEYAY 177 Query: 191 QPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMFHC 250 Q P DEWQ+I+L YTSGTT PKGVV HHRGAY+ A+SN L W M VYLWTLPMFHC Sbjct: 178 QLPEDEWQAISLNYTSGTTGKPKGVVYHHRGAYLNAVSNILEWAMPHHPVYLWTLPMFHC 237 Query: 251 NGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTILP 310 NGWCFPW++A +G S+CLR+V K++ +I KVTHF AP+VLN +VN P E Sbjct: 238 NGWCFPWTIAANAGVSVCLRRVDPKKIMDLIVDEKVTHFSGAPIVLNGLVNLPAEQK-RA 296 Query: 311 LPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPETQA 370 H V V TAGAAPP SV+ + + G V H YGL+E YGP TVCAW+ WD LP E +A Sbjct: 297 FDHPVKVTTAGAAPPASVIAGVEKLGIEVTHVYGLTEVYGPVTVCAWREAWDELPLEERA 356 Query: 371 KLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKETFA 430 ++ ARQGVRY +E L V D T +PVP DG+T GEI+ RGN VMKGYLKN A ++ Sbjct: 357 RIKARQGVRYHMLEALCVADPNTLEPVPKDGETIGEILMRGNNVMKGYLKNEAATEQALE 416 Query: 431 GGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARPD 490 GGW+H+GD+AV HPD YIEIKDRSKD+IISGGENIS++EVE+ +Y HPAV EA+VVA+PD Sbjct: 417 GGWYHTGDLAVWHPDGYIEIKDRSKDIIISGGENISTIEVEDAIYSHPAVEEAAVVAKPD 476 Query: 491 ERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGKIQ 550 E+W E+PCAFV LK Y + + DI++ CR+ L + VPK+V+F LPKT+TGKIQ Sbjct: 477 EKWGETPCAFVKLKVGYGEVTE----ADIIEHCRQHLARFKVPKTVIFTELPKTSTGKIQ 532 Query: 551 KHILRTKAK 559 K +LR +A+ Sbjct: 533 KFVLREEAR 541 Lambda K H 0.319 0.134 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 914 Number of extensions: 43 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 569 Length of database: 543 Length adjustment: 36 Effective length of query: 533 Effective length of database: 507 Effective search space: 270231 Effective search space used: 270231 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory