Alignments for a candidate for HPD in Haloechinothrix alba DSM 45207

GapMind for catabolism of small carbon sources

Alignments for a candidate for HPD in Haloechinothrix alba DSM 45207

Align 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (characterized)
to candidate WP_089299872.1 CHB84_RS02105 4-hydroxyphenylpyruvate dioxygenase

Query= BRENDA::Q53586
         (381 letters)



>NCBI__GCF_900188115.1:WP_089299872.1
          Length = 399

 Score =  491 bits (1265), Expect = e-143
 Identities = 249/377 (66%), Positives = 292/377 (77%), Gaps = 9/377 (2%)

Query: 10  DTARQADPFPVKGMDAVVFAVGNAKQAAHYYSTAFGMQLVAYSGPENGSRETASYVLTNG 69
           D     DPFPV+ MDAVVF VGNA QAA +Y  AFGM LVAYSGPE G+ +  ++VLT+G
Sbjct: 27  DHDETTDPFPVRAMDAVVFVVGNATQAAWFYQAAFGMTLVAYSGPETGNSDHKAFVLTSG 86

Query: 70  SARFVLTSVIKPATPWGHFLADHVAEHGDGVVDLAIEVPDARAAHAYAIEHGARSVAEPY 129
           SARFV+   ++P++P    L DH   HGDGVVDLA+EV D      +A   GA  + EP+
Sbjct: 87  SARFVVKGGVQPSSP----LLDHHRRHGDGVVDLALEVADVDRCIEHARAMGATVLDEPH 142

Query: 130 ELKDEHGTVVLAAIATYGKTRHTLVDRTGYDGPYLPGYVA---AAPIVEPPAHRTFQAID 186
           +  DEHGTV   AIATYG+TRHTLVDR+ Y GPYLPGYVA   A    E    R FQA+D
Sbjct: 143 DEHDEHGTVRTGAIATYGETRHTLVDRSRYAGPYLPGYVARHGAIERAEGAPRRLFQAVD 202

Query: 187 HCVGNVELGRMNEWVGFYNKVMGFTNMKEFVGDDIATEYSALMSKVVADGTLKVKFPINE 246
           HCVGNVELG M+ WV FY +VMGF NM EFVGDDIATEYSALMSKVVA+G  +VKFP+NE
Sbjct: 203 HCVGNVELGNMDYWVDFYRRVMGFVNMAEFVGDDIATEYSALMSKVVANGNHRVKFPLNE 262

Query: 247 PALAKKKSQIDEYLEFYGGAGVQHIALNTGDIVETVRTMRAAGVQFLDTPDSYYD--TLG 304
           PA+AKK+SQIDEYL++YGGAG QHIAL T DI+ TVR MR AGV+FL+ PDSYYD   L 
Sbjct: 263 PAVAKKRSQIDEYLDYYGGAGCQHIALATNDIISTVRHMRKAGVEFLNVPDSYYDDPELR 322

Query: 305 EWVGDTRVPVDTLRELKILADRDEDGYLLQIFTKPVQDRPTVFFEIIERHGSMGFGKGNF 364
             +G+ RVP++TL+E  IL DRDEDGYLLQ+FTKP+ DRPTVFFE+IERHGS+GFGKGNF
Sbjct: 323 ARIGEVRVPIETLQEHGILVDRDEDGYLLQLFTKPIGDRPTVFFELIERHGSLGFGKGNF 382

Query: 365 KALFEAIEREQEKRGNL 381
           KALFEAIEREQ++RGNL
Sbjct: 383 KALFEAIEREQQRRGNL 399


Lambda     K      H
   0.318    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 657
Number of extensions: 27
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 381
Length of database: 399
Length adjustment: 30
Effective length of query: 351
Effective length of database: 369
Effective search space:   129519
Effective search space used:   129519
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_089299872.1 CHB84_RS02105 (4-hydroxyphenylpyruvate dioxygenase)
to HMM TIGR01263 (hppD: 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01263.hmm
# target sequence database:        /tmp/gapView.16242.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01263  [M=353]
Accession:   TIGR01263
Description: 4HPPD: 4-hydroxyphenylpyruvate dioxygenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   6.3e-131  423.0   0.0   8.2e-131  422.6   0.0    1.1  1  lcl|NCBI__GCF_900188115.1:WP_089299872.1  CHB84_RS02105 4-hydroxyphenylpyr


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_900188115.1:WP_089299872.1  CHB84_RS02105 4-hydroxyphenylpyruvate dioxygenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  422.6   0.0  8.2e-131  8.2e-131       2     353 .]      39     399 .]      38     399 .] 0.98

  Alignments for each domain:
  == domain 1  score: 422.6 bits;  conditional E-value: 8.2e-131
                                 TIGR01263   2 gfdfvefavgdakqaakalveklGfeavak...etgsrekastvlrqgeitlvltaelsssseaaafla 67 
                                               ++d v f+vg+a+qaa  ++ ++G+++va+   etg++++++ vl++g++++v++   ++ss++ ++ +
  lcl|NCBI__GCF_900188115.1:WP_089299872.1  39 AMDAVVFVVGNATQAAWFYQAAFGMTLVAYsgpETGNSDHKAFVLTSGSARFVVKGGVQPSSPLLDHHR 107
                                               5899***************************************************************** PP

                                 TIGR01263  68 kHGdgvkdvafevedveaafeaavergaeavsapee.ed.ekevklaaikgiGdvvltlveregekgsi 134
                                               +HGdgv d+a+ev+dv+ + e+a + ga+++++p++ +d +++v++ ai ++G++++tlv+r++++g +
  lcl|NCBI__GCF_900188115.1:WP_089299872.1 108 RHGDGVVDLALEVADVDRCIEHARAMGATVLDEPHDeHDeHGTVRTGAIATYGETRHTLVDRSRYAGPY 176
                                               **********************************997668***************************** PP

                                 TIGR01263 135 lpgfeevsekaalkekledvgleaiDHvvgnvergelekvaefyekilgfkeiksfd...ikteasaLk 200
                                               lpg+++ + + + +e  +   ++a+DH+vgnve g+++++++fy++++gf ++++f    i te+saL+
  lcl|NCBI__GCF_900188115.1:WP_089299872.1 177 LPGYVARHGAIERAEGAPRRLFQAVDHCVGNVELGNMDYWVDFYRRVMGFVNMAEFVgddIATEYSALM 245
                                               ********************************************************8899********* PP

                                 TIGR01263 201 SkvlasaegkvklplnepaskkkksQIeeyleeyeGaGvQHlAlntedivktveelrargveflkipet 269
                                               Skv+a+ +++vk+plnepa  kk+sQI+eyl++y GaG QH+Al t+di++tv+++r+ gvefl++p++
  lcl|NCBI__GCF_900188115.1:WP_089299872.1 246 SKVVANGNHRVKFPLNEPAVAKKRSQIDEYLDYYGGAGCQHIALATNDIISTVRHMRKAGVEFLNVPDS 314
                                               ********************************************************************* PP

                                 TIGR01263 270 YYdn..lkervkklvkedleelkelkiLvDrdeeGlLLQiFtkpvvdrgtlFfEiIqRkgakGFGegNf 336
                                               YYd+  l+ r+++ v+ ++e+l+e++iLvDrde+G+LLQ+Ftkp+ dr+t+FfE+I+R+g+ GFG+gNf
  lcl|NCBI__GCF_900188115.1:WP_089299872.1 315 YYDDpeLRARIGE-VRVPIETLQEHGILVDRDEDGYLLQLFTKPIGDRPTVFFELIERHGSLGFGKGNF 382
                                               ***6337788886.******************************************************* PP

                                 TIGR01263 337 kaLfeaiEreqekrgvl 353
                                               kaLfeaiEreq++rg+l
  lcl|NCBI__GCF_900188115.1:WP_089299872.1 383 KALFEAIEREQQRRGNL 399
                                               ***************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (353 nodes)
Target sequences:                          1  (399 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 11.84
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources