Alignments for a candidate for pccA in Haloechinothrix alba DSM 45207

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccA in Haloechinothrix alba DSM 45207

Align Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 (characterized)
to candidate WP_089302466.1 CHB84_RS16245 pyruvate carboxylase

Query= SwissProt::I3R7G3
         (601 letters)



>NCBI__GCF_900188115.1:WP_089302466.1
          Length = 1124

 Score =  402 bits (1033), Expect = e-116
 Identities = 222/448 (49%), Positives = 293/448 (65%), Gaps = 7/448 (1%)

Query: 1   MFSKVLVANRGEIAVRVMRACEELGVRTVAVYSEADKHGGHVRYADEAYNIG-PARAADS 59
           MF KVLVANRGEIA+R  RA  ELG  TVAV+   D++  H   ADE++ IG P     +
Sbjct: 1   MFRKVLVANRGEIAIRAFRAGYELGAGTVAVFPHEDRNSLHRLKADESHQIGEPGHPVRA 60

Query: 60  YLDHESVIEAARKADADAIHPGYGFLAENAEFARKVEDSEFTWVGPSADAMERLGEKTKA 119
           YL  E V+ AA  ADADA++PGYGFL+EN + AR  E++  T+VGP A+ +E  G K++A
Sbjct: 61  YLSVEEVVRAALSADADAVYPGYGFLSENPDLARACEEAGITFVGPPAEILELTGNKSRA 120

Query: 120 RSLMQDADVPVVPGTTEPADSAEDVKAVADDYGYPVAIKAEGGGGGRGLKVVHSEDEVDG 179
               ++A +PV+ G++ P+D  +++ A A++ G+PV +KA  GGGGRG++ V    ++  
Sbjct: 121 VQAAREAGIPVL-GSSRPSDDVDELVAAAEEIGFPVFVKAIAGGGGRGMRKVTEPAQLRE 179

Query: 180 QFETAKREGEAYFDNASVYVEKYLEAPRHIEVQILADEHGNVRHLGERDCSLQRRHQKVI 239
             E A RE E+ F + SV+VE+ +  PRHIEVQILAD  G V HL ERDCS+QRRHQKVI
Sbjct: 180 SIEAASREAESAFGDPSVFVERAVVNPRHIEVQILADGTGEVVHLYERDCSVQRRHQKVI 239

Query: 240 EEAPSPALSEDLRERIGEAARRGVRAAEYTNAGTVEFLV-EDGEFYFMEVNTRIQVEHTV 298
           E AP+P L+ +LRERI   A R  R   Y NAGTVEFLV E GE  F+E+N RIQVEHTV
Sbjct: 240 ELAPAPNLAPELRERICADAARLARRIGYRNAGTVEFLVDESGEHVFIEMNPRIQVEHTV 299

Query: 299 TEEVTGLDVVKWQLRVAAGE---ELDFSQDDVEIEGHSMEFRINAEAPEKEFAPATGTLS 355
           TEEVT +D+V  QLR+AAGE   +L  +QD + + G +++ RI  E P  EF P  G +S
Sbjct: 300 TEEVTDVDLVAAQLRIAAGESLADLGLTQDVIYLRGAALQCRITTEDPANEFRPDVGMIS 359

Query: 356 TYDPPGGIGIRMDDAVR-QGDEIGGDYDSMIAKLIVTGSDREEVLVRAERALNEFDIEGL 414
            Y  PGG GIR+D      G EI   +DSM+ KL   G D +  + RA RA+ EF I G+
Sbjct: 360 AYRSPGGSGIRLDGGTAFAGTEISAHFDSMLVKLTCRGRDFDTAVARARRAVAEFRIRGV 419

Query: 415 RTVIPFHRLMLTDEAFREGSHTTKYLDE 442
            T IPF R +L D  FR G  TT ++++
Sbjct: 420 ATNIPFLRAVLDDPDFRAGRVTTSFIED 447



 Score = 58.2 bits (139), Expect = 2e-12
 Identities = 31/67 (46%), Positives = 43/67 (64%), Gaps = 1/67 (1%)

Query: 535  VAAEMQGTILAVEVDEGDDVEPGDTVCILEAMKMENDVVAERGGTVSQVLVGEGDSVDMG 594
            VAA   G ++ + V EGD VE G TV  +EAMKME  + A +GG VS++ +     V+ G
Sbjct: 1059 VAAPFAG-VVTLSVSEGDTVEAGATVATIEAMKMEAAITASKGGRVSRLAINSVQQVEGG 1117

Query: 595  DVLLVLE 601
            D+LLV+E
Sbjct: 1118 DLLLVVE 1124


Lambda     K      H
   0.312    0.132    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1434
Number of extensions: 64
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 601
Length of database: 1124
Length adjustment: 42
Effective length of query: 559
Effective length of database: 1082
Effective search space:   604838
Effective search space used:   604838
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources