Align aconitate hydratase (EC 4.2.1.3) (characterized)
to candidate WP_090438471.1 BLS63_RS00850 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase
Query= BRENDA::P36683 (865 letters) >NCBI__GCF_900100495.1:WP_090438471.1 Length = 869 Score = 1373 bits (3554), Expect = 0.0 Identities = 674/859 (78%), Positives = 755/859 (87%), Gaps = 3/859 (0%) Query: 1 MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEFLLDLLTNRVPPGVDEA 60 MLE YRKHVAERAA+GI P+PL+A Q A LVELLKNPPAGEE FL+DL+TNRVP GVDEA Sbjct: 1 MLEAYRKHVAERAAQGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPAGVDEA 60 Query: 61 AYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPLIDALDDAKLAPIAAKALS 120 AYVKAGFL+A+AKGE SPLL+ +A+ELLGTMQGGYNI L++ LD+A+LA +AA+ L Sbjct: 61 AYVKAGFLSALAKGEVSSPLLSKTRAVELLGTMQGGYNITTLVELLDNAELAAVAAEQLK 120 Query: 121 HTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRPALAEKLTVTVFKVTGETNTD 180 HTLLMFD F+DV EKAKAGN AK V+ SWA+ EWF NR A+ EK+T+TVFKVTGETNTD Sbjct: 121 HTLLMFDAFHDVAEKAKAGNANAKAVLASWAEGEWFQNRAAVPEKVTLTVFKVTGETNTD 180 Query: 181 DLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVGPIKQIEALQQKGFPLAYVGDVV 240 DLSPAPDAWSRPDIPLHALAMLK AR+GIEPDQPG VGPIKQ+EAL+ KGFP+AYVGDVV Sbjct: 181 DLSPAPDAWSRPDIPLHALAMLKMARDGIEPDQPGSVGPIKQMEALKAKGFPVAYVGDVV 240 Query: 241 GTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGGKIAPIFFNTMEDAGALPIEVDVSN 300 GTGSSRKSATNSVLWF GDDIP+VPNKR GG C G KIAPIF+NTMEDAGALPIE D +N Sbjct: 241 GTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGTKIAPIFYNTMEDAGALPIEFDCTN 300 Query: 301 LNMGDVIDVYPYKGEVRNHETGELLATFELKTDVLIDEVRAGGRIPLIIGRGLTTKAREA 360 L MGDVIDVYP KGEVR H + EL+ +F+LKTDVL+DEVRAGGRIPLI+GRGLT KAR Sbjct: 301 LAMGDVIDVYPIKGEVRRHGSDELVTSFQLKTDVLLDEVRAGGRIPLIVGRGLTEKARAE 360 Query: 361 LGLPHSDVFRQAKDVAESDRGFSLAQKMVGRACGV---KGIRPGAYCEPKMTSVGSQDTT 417 LGL S +F + + A+S +GF+LAQKMVGRACG+ +G+RPG YCEPKMT+VGSQDTT Sbjct: 361 LGLAPSTLFNKPESPADSGKGFTLAQKMVGRACGLAEGQGVRPGTYCEPKMTTVGSQDTT 420 Query: 418 GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDG 477 GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKP+DV THHTLPDFI RGGVSLRPGDG Sbjct: 421 GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIRTRGGVSLRPGDG 480 Query: 478 VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK 537 +IHSWLNRMLLPDTVGTGGDSHTRFP+GISFPAGSGLVAFAAATGVMPLDMPESVLVRFK Sbjct: 481 IIHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK 540 Query: 538 GKMQPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTD 597 GK+QPGITLRDLVHAIP YAIK GLLTVEKKGK N FSGRILEIEGL +L VEQAFEL+D Sbjct: 541 GKLQPGITLRDLVHAIPYYAIKAGLLTVEKKGKINAFSGRILEIEGLDELTVEQAFELSD 600 Query: 598 ASAERSAAGCTIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPEL 657 ASAERSAAGCTIKL ++ I EYL SNI LL+WMI EGYGD RTLERR + ME WLANP+L Sbjct: 601 ASAERSAAGCTIKLPEKAIAEYLQSNITLLRWMIGEGYGDARTLERRAKAMEAWLANPQL 660 Query: 658 LEADADAEYAAVIDIDLADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHF 717 LEAD DAEYA +I+IDLA+I EP+LCAPNDPDDAR LS+V GEKIDEVFIGSCMTNIGHF Sbjct: 661 LEADKDAEYAEIIEIDLAEINEPVLCAPNDPDDARLLSSVAGEKIDEVFIGSCMTNIGHF 720 Query: 718 RAAGKLLDAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQA 777 RAAGKLLD KG +PTRLW+APPT+MDA QLTEEGYY ++GK+GAR+E+PGCSLCMGNQA Sbjct: 721 RAAGKLLDKVKGGIPTRLWLAPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQA 780 Query: 778 RVADGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTA 837 RV G+TVVSTSTRNFPNRLG NV+LASAELAAVA++IGKLP+ EEY Y +D A Sbjct: 781 RVQTGSTVVSTSTRNFPNRLGDATNVYLASAELAAVASIIGKLPSVEEYMQYAKDIDSMA 840 Query: 838 VDTYRYLNFNQLSQYTEKA 856 D YRYL+F+Q++++ E A Sbjct: 841 ADVYRYLSFDQIAEFREAA 859 Lambda K H 0.317 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2177 Number of extensions: 78 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 865 Length of database: 869 Length adjustment: 42 Effective length of query: 823 Effective length of database: 827 Effective search space: 680621 Effective search space used: 680621 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory