Align 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (EC 4.2.1.117) (characterized)
to candidate WP_090439551.1 BLS63_RS00675 Fe/S-dependent 2-methylisocitrate dehydratase AcnD
Query= BRENDA::Q8EJW3 (867 letters) >NCBI__GCF_900100495.1:WP_090439551.1 Length = 869 Score = 1406 bits (3640), Expect = 0.0 Identities = 694/866 (80%), Positives = 769/866 (88%), Gaps = 8/866 (0%) Query: 5 MNTQYRKPLPGTALDYFDTREAIEAIAPGAYAKLPYTSRVLAENLVRRCEPEMLTASLKQ 64 MN+QYRKPL GT LDYFDTREA++AIAPGAYAKLPYTSRVLAE LVRRCEP+ LT SLKQ Sbjct: 1 MNSQYRKPLAGTVLDYFDTREAVDAIAPGAYAKLPYTSRVLAEQLVRRCEPDALTDSLKQ 60 Query: 65 IIESKQELDFPWFPARVVCHDILGQTALVDLAGLRDAIAAKGGDPAQVNPVVPTQLIVDH 124 +IE K++LDFPW+PARVVCHDILGQTALVDLAGLRDAIA KGGDP++VNPVVPTQLIVDH Sbjct: 61 LIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEKGGDPSKVNPVVPTQLIVDH 120 Query: 125 SLAVEYGGFDKDAFAKNRAIEDRRNEDRFHFINWTQKAFKNIDVIPQGNGIMHQINLERM 184 SLAVE GFD DAFAKNRAIEDRRNEDRFHFI WT+ AFKN+DVIP GNGIMHQINLE+M Sbjct: 121 SLAVEAPGFDPDAFAKNRAIEDRRNEDRFHFIEWTKTAFKNVDVIPAGNGIMHQINLEKM 180 Query: 185 SPVIHARNGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAESVMLGRASYMRLPDIIGV 244 SPVI AR GVAFPDT VGTDSHTPHVDALGVIAIGVGGLEAE+VMLG S MRLPDI+GV Sbjct: 181 SPVIQARGGVAFPDTCVGTDSHTPHVDALGVIAIGVGGLEAETVMLGLPSMMRLPDIVGV 240 Query: 245 ELTGKPQPGITATDIVLALTEFLRAQKVVSSYLEFFGEGAEALTLGDRATISNMTPEFGA 304 +LTGK QPGITATDIVLA+TEFLR ++VV +Y+EFFGEGA++L++GDRATISNM PE+GA Sbjct: 241 KLTGKRQPGITATDIVLAITEFLRKERVVGAYVEFFGEGADSLSIGDRATISNMCPEYGA 300 Query: 305 TAAMFYIDQQTLDYLTLTGREAEQVKLVETYAKTAGLWSDDLKQAVYPRTLHFDLSSVVR 364 TA+MFYIDQQT+DYL LTGRE EQV LVE YAK GLW+D L A Y R L FDLS+VVR Sbjct: 301 TASMFYIDQQTIDYLKLTGREPEQVALVENYAKLTGLWADALVTAEYERVLEFDLSTVVR 360 Query: 365 TIAGPSNPHARVPTSELAARGI-------SGEVENEPGLMPDGAVIIAAITSCTNTSNPR 417 +AGPSNPH R+PTS L RGI SG+VE GL+PDGAVIIAAITSCTNTSNPR Sbjct: 361 NMAGPSNPHKRLPTSALHERGIADEAKLASGKVEEAEGLLPDGAVIIAAITSCTNTSNPR 420 Query: 418 NVIAAGLLARNANAKGLTRKPWVKTSLAPGSKAVQLYLEEANLLPELESLGFGIVGFACT 477 NV+AAGLLA+ ANA GL RKPWVKTS APGSK +LYLE+A LL ELE LGFGIVG+ACT Sbjct: 421 NVVAAGLLAKKANALGLLRKPWVKTSFAPGSKVAKLYLEDAGLLSELEQLGFGIVGYACT 480 Query: 478 TCNGMSGALDPVIQQEVIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG 537 TCNGMSGALDPVIQQE+IDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG Sbjct: 481 TCNGMSGALDPVIQQEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG 540 Query: 538 TIRFDIEKDVLGLDKDGKPVRLINIWPSDAEIDAVIAASVKPEQFRKVYEPMFDLSVDYG 597 T+RFDIE+DVLG DK+G + L ++WPSD EIDA++AASVKPEQF+++Y PMFDL Sbjct: 541 TVRFDIEQDVLGTDKNGNLITLKDLWPSDEEIDAIVAASVKPEQFKQIYIPMFDLGT-IQ 599 Query: 598 DKVSPLYDWRPQSTYIRRPPYWEGALAGERTLKGMRPLAVLGDNITTDHLSPSNAIMMDS 657 + SPLYDWRP STYIRRPPYWEGALAGERTLKGMRPLA+L DNITTDHLSPSNAI++DS Sbjct: 600 EAESPLYDWRPMSTYIRRPPYWEGALAGERTLKGMRPLAILPDNITTDHLSPSNAILLDS 659 Query: 658 AAGEYLHKMGLPEEDFNSYATHRGDHLTAQRATFANPKLKNEMAIVDGKVKQGSLARIEP 717 AAGEYLHKMGLPEEDFNSYATHRGDHLTAQRATFANP+L NEMA+VDG VK+GSLAR+EP Sbjct: 660 AAGEYLHKMGLPEEDFNSYATHRGDHLTAQRATFANPQLVNEMAVVDGVVKKGSLARVEP 719 Query: 718 EGIVTRMWEAIETYMDRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHR 777 EG V RMWEAIETYM+RKQ LII+AGADYGQGSSRDWAAKGVRLAGVE IVAEGFERIHR Sbjct: 720 EGKVMRMWEAIETYMNRKQNLIIVAGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIHR 779 Query: 778 TNLVGMGVLPLEFKAGENRATYGIDGTEVFDVIGSIAPRADLTVIITRKNGERVEVPVTC 837 TNLVGMGVLP+EFK G R T G+DG+E +D+ G ++PR +L ++I R+NGE +VPVTC Sbjct: 780 TNLVGMGVLPVEFKPGTTRLTLGLDGSETYDIEGDVSPRCELILVIHRRNGEVTQVPVTC 839 Query: 838 RLDTAEEVSIYEAGGVLQRFAQDFLE 863 RLDTA EV +Y+AGGVLQRFAQDFLE Sbjct: 840 RLDTAAEVHVYKAGGVLQRFAQDFLE 865 Lambda K H 0.318 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2246 Number of extensions: 81 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 867 Length of database: 869 Length adjustment: 42 Effective length of query: 825 Effective length of database: 827 Effective search space: 682275 Effective search space used: 682275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory