Alignments for a candidate for kdgD in Pseudomonas benzenivorans DSM 8628

GapMind for catabolism of small carbon sources

Alignments for a candidate for kdgD in Pseudomonas benzenivorans DSM 8628

Align 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) (characterized)
to candidate WP_090439667.1 BLS63_RS03175 5-dehydro-4-deoxyglucarate dehydratase

Query= reanno::pseudo1_N1B4:Pf1N1B4_1110
         (303 letters)



>NCBI__GCF_900100495.1:WP_090439667.1
          Length = 303

 Score =  525 bits (1353), Expect = e-154
 Identities = 265/303 (87%), Positives = 280/303 (92%)

Query: 1   MNPQELKSILSSGLLSFPVTDFNAQGDFHRAGYIKRLEWLAPYGASALFAAGGTGEFFSL 60
           MNPQELKSILSSGLLSFPVTDF+A GDF    Y +RLEWLAPYGASALFAAGGTGEFFSL
Sbjct: 1   MNPQELKSILSSGLLSFPVTDFDAAGDFRADTYARRLEWLAPYGASALFAAGGTGEFFSL 60

Query: 61  AASEYSEIIKTAVDTCATSVPILAGVGGATRQAIEYAQEAERLGAKGLLLLPHYLTEASQ 120
           A  EYS +IKTAVDTCA SVPILAGVGG TR AI+YAQEAERLGAKGLLLLPHYLTEASQ
Sbjct: 61  APDEYSAVIKTAVDTCAGSVPILAGVGGPTRLAIQYAQEAERLGAKGLLLLPHYLTEASQ 120

Query: 121 DGVAAHVEAVCKSVKIGVVVYNRNVCRLTAPLLERLAERCPNLIGYKDGLGDIELMVSIR 180
           +GVAAHVE VCKSVKIGVVVYNRNVCRLTAPLLE+LAERCPNLIGYKDGLGDIELMVSIR
Sbjct: 121 EGVAAHVEQVCKSVKIGVVVYNRNVCRLTAPLLEQLAERCPNLIGYKDGLGDIELMVSIR 180

Query: 181 RRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKTAMDFYHAIAREDHATVGK 240
           RRLG+RFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIP+TAMDFY AIAR+D ATVG+
Sbjct: 181 RRLGERFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPRTAMDFYRAIARDDQATVGR 240

Query: 241 IIDDFFLPYLDIRNRKAGYAVSIVKAGAKIAGYDAGPVRAPLTDLTGEEYEMLAALIDKQ 300
           +ID+FFLPYLDIRNR  GYAVSIVKAGA+I GYDAGPVRAPLTDL  +EYE LA LI+ Q
Sbjct: 241 LIDEFFLPYLDIRNRCKGYAVSIVKAGARIVGYDAGPVRAPLTDLLADEYEALARLIEAQ 300

Query: 301 GAQ 303
           GAQ
Sbjct: 301 GAQ 303


Lambda     K      H
   0.320    0.137    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 451
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 303
Length of database: 303
Length adjustment: 27
Effective length of query: 276
Effective length of database: 276
Effective search space:    76176
Effective search space used:    76176
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate WP_090439667.1 BLS63_RS03175 (5-dehydro-4-deoxyglucarate dehydratase)
to HMM TIGR03249 (kdgD: 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03249.hmm
# target sequence database:        /tmp/gapView.9770.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03249  [M=299]
Accession:   TIGR03249
Description: KdgD: 5-dehydro-4-deoxyglucarate dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.6e-135  435.4   0.0   5.2e-135  435.3   0.0    1.0  1  lcl|NCBI__GCF_900100495.1:WP_090439667.1  BLS63_RS03175 5-dehydro-4-deoxyg


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_900100495.1:WP_090439667.1  BLS63_RS03175 5-dehydro-4-deoxyglucarate dehydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  435.3   0.0  5.2e-135  5.2e-135       1     297 [.       3     299 ..       3     301 .. 0.99

  Alignments for each domain:
  == domain 1  score: 435.3 bits;  conditional E-value: 5.2e-135
                                 TIGR03249   1 pqelkkkigsGllsfPvtpfdadgsldeaalrenieflldedlealfvagGtGeffsltkaeveqvvev 69 
                                               pqelk  ++sGllsfPvt+fda g+++ +++++++e+l++++++alf+agGtGeffsl + e+++v++ 
  lcl|NCBI__GCF_900100495.1:WP_090439667.1   3 PQELKSILSSGLLSFPVTDFDAAGDFRADTYARRLEWLAPYGASALFAAGGTGEFFSLAPDEYSAVIKT 71 
                                               89******************************************************************* PP

                                 TIGR03249  70 aveaakgkvPvlagvGgnlsvaieiaraaekkGadGllllPpylieaeqeGlaayvkaviesvdlgviv 138
                                               av++++g vP+lagvGg ++ ai++a++ae+ Ga+GllllP+yl+ea+qeG+aa+v++v++sv++gv+v
  lcl|NCBI__GCF_900100495.1:WP_090439667.1  72 AVDTCAGSVPILAGVGGPTRLAIQYAQEAERLGAKGLLLLPHYLTEASQEGVAAHVEQVCKSVKIGVVV 140
                                               ********************************************************************* PP

                                 TIGR03249 139 yqrdnavleadtlerlaerlpnlvGfkdGiGdiekvieitqklGdrllylgGlPtaevtalaylalGvt 207
                                               y+r+ + l+a+ le+laer+pnl+G+kdG+Gdie +++i+++lG+r+ ylgGlPtaev+a+ay+alGv 
  lcl|NCBI__GCF_900100495.1:WP_090439667.1 141 YNRNVCRLTAPLLEQLAERCPNLIGYKDGLGDIELMVSIRRRLGERFSYLGGLPTAEVYAAAYKALGVP 209
                                               ********************************************************************* PP

                                 TIGR03249 208 syssaifnfiPkiarkfyealrkgdeatvkeilkevilPiveirnrkkGyavslikaGlevvGrdvgpv 276
                                               +yssa+fnfiP++a++fy+a++++d+atv ++++e++lP++ irnr kGyavs++kaG+++vG d+gpv
  lcl|NCBI__GCF_900100495.1:WP_090439667.1 210 VYSSAVFNFIPRTAMDFYRAIARDDQATVGRLIDEFFLPYLDIRNRCKGYAVSIVKAGARIVGYDAGPV 278
                                               ********************************************************************* PP

                                 TIGR03249 277 raPlvdlekeelaeleellkk 297
                                               raPl+dl  +e++ l++l+++
  lcl|NCBI__GCF_900100495.1:WP_090439667.1 279 RAPLTDLLADEYEALARLIEA 299
                                               ******************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (299 nodes)
Target sequences:                          1  (303 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 11.44
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources