Align α,α-trehalase (MSMEG_4535;MSMEG4528) (EC 3.2.1.28) (characterized)
to candidate WP_090441135.1 BLS63_RS05730 glycoside hydrolase family 15 protein
Query= CAZy::ABK72415.1 (668 letters) >NCBI__GCF_900100495.1:WP_090441135.1 Length = 598 Score = 238 bits (606), Expect = 8e-67 Identities = 180/617 (29%), Positives = 280/617 (45%), Gaps = 40/617 (6%) Query: 44 DYGFLSDCETTCLISSAGSVEWLCVPRPDSPSVFGAIL--DRGAGHFRLGPYGVSVPAAR 101 + G + +C L+++ G + W C P D F +L D+ G G+S + Sbjct: 9 ELGLIGNCRVAALVNTLGRLVWWCYPNFDGDPAFSRLLAGDQEKGFSDCLLDGMS-SSRS 67 Query: 102 RYLPGSLILETTWQTHTGWLIVRDALVMGPWHDIDTRSRTHRRTPMDWDAEHILLRTVRC 161 YL + I+ TT Q G + D R + R L R + Sbjct: 68 EYLRNTAIISTTLQDAAGNAVRIT--------DFAPRFLQYGRNFRPAQ----LCRLIEP 115 Query: 162 VSGTVELVMSCEPAFDYHRVSATWEYSGPAYGEAIARASRNPDSHPTLRLTTNLRIGIEG 221 + G + + P T Y P + A + R T+RLTT+ + Sbjct: 116 LQGLPRVTLRMRP---------THGYGKPCHAVAGSSHIRYLGGADTIRLTTDAPLSYIL 166 Query: 222 REARARTRLTEGDNVFVALSWSKHPAPQTYEEAADKMWKTSEAWRQWINVGDFPDHPWRA 281 RE LT ++ + + AP+ + K +TS W +W+ P W+ Sbjct: 167 RETPFA--LTRPISLVMGVDEPLDDAPEELVKTFLK--RTSACWHEWVRALAIP-FEWQE 221 Query: 282 YLQRSALTLKGLTYSPTGALLAAPTTSLPETPQGERNWDYRYSWIRDSTFALWGLYTLGL 341 + R+A+TLK + TGA++AA TTS+PE P +RNWDYRY W+RD+ F + L LG Sbjct: 222 VVIRAAITLKLCNFEETGAIIAAVTTSIPEAPGSQRNWDYRYCWLRDAYFVIQALNRLGA 281 Query: 342 DREADDFFSFIADVSGANNGERHPLQVMYGVGGERSLVEEELHHLSGYDNSRPVRIGNGA 401 R + + FI V A G L+ +YG+ + L E L+G+ PVR+GN A Sbjct: 282 TRTMERYVDFITTV--ATGGAE--LKPLYGIVPDLDLSERIEPDLAGFLGHGPVRVGNQA 337 Query: 402 YNQRQHDIWGTMLDSV---YLHAKSREQIPDALWPVLKNQVEEAIKHWKEPDRGIWEVRG 458 Q QHD++G+++ +V ++ + + + + +L++ A EPD GIWE RG Sbjct: 338 VEQIQHDVFGSVVLAVAQSFVDERLPKPGDEGMLRLLESLAARAACFAFEPDAGIWEYRG 397 Query: 459 EPQHFTSSKIMCWVALDRGSKLAELQGEKSYAQQWRAIAEEIKADVLARG-VDKRGVLTQ 517 + T S ++CWVA DR ++A G A+ W A ++ +L ++R T Sbjct: 398 RQRVHTHSALLCWVACDRVGRIAARLGLAQEARTWHEEARRLRERILHDAWSERRQSFTG 457 Query: 518 RYGDDALDASLLLAVLTRFLPADDPRIRATVLAIADELTEDGLVLRYRVEETDDGLAGEE 577 +G D LDAS+LL + A DPR ATV + EL +G +LRY T D E Sbjct: 458 TFGHDDLDASVLLMNELGIIDAGDPRFIATVECVGRELNVNGHLLRY---ATADDFGLPE 514 Query: 578 GTFTICSFWLVSALVEIGEISRAKHLCERLLSFASPLHLYAEEIEPRTGRHLGNFPQAFT 637 F + FW + AL IG A+ L++ + L AE++ P TG GN PQ ++ Sbjct: 515 TAFLVVKFWYLDALAAIGRRDEARERYAELVAARNTYGLLAEDLHPHTGEMWGNIPQTYS 574 Query: 638 HLALINAVVHVIRAEEE 654 L+N + + + EE Sbjct: 575 MAGLVNTAMRLSISWEE 591 Lambda K H 0.319 0.135 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1033 Number of extensions: 56 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 668 Length of database: 598 Length adjustment: 38 Effective length of query: 630 Effective length of database: 560 Effective search space: 352800 Effective search space used: 352800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory