Alignments for a candidate for liuA in Pseudomonas benzenivorans DSM 8628

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuA in Pseudomonas benzenivorans DSM 8628

Align Putative acyl-CoA dehydrogenase AidB; EC 1.3.99.- (characterized)
to candidate WP_090445290.1 BLS63_RS13640 DNA alkylation response protein

Query= SwissProt::P33224
         (541 letters)



>NCBI__GCF_900100495.1:WP_090445290.1
          Length = 549

 Score =  405 bits (1040), Expect = e-117
 Identities = 236/524 (45%), Positives = 308/524 (58%), Gaps = 10/524 (1%)

Query: 4   QTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGRLAN 63
           +TH VFNQ  PL++ NLY  D  L E   R G  W    + + G   G  E +  G LAN
Sbjct: 8   ETHEVFNQVPPLDDVNLYRIDLPLQEWTRRFGGDWAEARIDAYGALAG-GELMAAGFLAN 66

Query: 64  VNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAARFML 123
            N P    +D  G R+D V FHPA+H LM A   + + ++ W    R GA VARAA   L
Sbjct: 67  ENKPVFKSHDRFGHRIDLVEFHPAYHQLMSAAIEHGIPSMPWTAP-RPGAQVARAAMSYL 125

Query: 124 HAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLIGMG 183
           H+Q EAGS CP+TMT+A+ P L ++ P     W   +LS  YD   LP  QK G  IGM 
Sbjct: 126 HSQAEAGSGCPLTMTYASVPAL-KLQPDIAALWLPKILSREYDPRNLPIEQKNGATIGMA 184

Query: 184 MTEKQGGSDVMSNTTRAERL----EDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFF 239
           MTEKQGG+DV +NTTRA  +       +Y LVGHKWF S P  DA L LA T  GL+CF 
Sbjct: 185 MTEKQGGTDVRANTTRAYPVGAPGPGQAYELVGHKWFCSAPMCDAFLTLAYTDRGLTCFL 244

Query: 240 VPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTR 299
           +PR  PDG RN   ++RLK+KLGN SNAS EVE++ A+ W++G EG G+  I++M  +TR
Sbjct: 245 LPRHRPDGTRNQFYIQRLKNKLGNWSNASSEVEYRGALAWMIGEEGRGVPTIIEMVALTR 304

Query: 300 FDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLA 359
           FDC +GS A+MR+A + A +H   R V G  L +QPLM++VL+ +AL+ E   AL  RL 
Sbjct: 305 FDCMVGSSALMRQALTQAAHHCAYRQVGGRVLSEQPLMQNVLADLALESEAALALTMRLG 364

Query: 360 RAWDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMP 419
           +A D   + +E  +ARL T   K+ ICKR    + EA E +GG GY E+S LPRLYRE P
Sbjct: 365 QALDNAHEQQEDKFARLVTAVGKYWICKRAPAMINEAAECMGGAGYVEDSILPRLYREAP 424

Query: 420 VNSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKPAE 479
           VNS WEGSGN+ CLDVLR L+K+ GV D L     +  G D      ++RLQ   R  AE
Sbjct: 425 VNSTWEGSGNVQCLDVLRALSKEPGVLDALFNELGDGHG-DARLKAHIQRLQADFRDSAE 483

Query: 480 --ELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGV 521
                R++T  + L      +L+  +  ++ A+    L   G V
Sbjct: 484 IQYRARQLTEDVALALQAKLLLEAGNATVSDAFIASRLGEGGRV 527


Lambda     K      H
   0.324    0.138    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 676
Number of extensions: 23
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 541
Length of database: 549
Length adjustment: 35
Effective length of query: 506
Effective length of database: 514
Effective search space:   260084
Effective search space used:   260084
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources