Align ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized)
to candidate WP_090447186.1 BLS63_RS18125 ABC transporter ATP-binding protein
Query= reanno::WCS417:GFF4321 (386 letters) >NCBI__GCF_900100495.1:WP_090447186.1 Length = 386 Score = 631 bits (1628), Expect = 0.0 Identities = 321/386 (83%), Positives = 345/386 (89%) Query: 1 MATLELRNVNKTYGAGLPDTLKNIELSIKEGEFLILVGPSGCGKSTLMNCIAGLETITGG 60 MATLELRNVNKTYG+GLPDTLKNIE++I GEFLILVGPSGCGKSTLMNCIAGLE I+GG Sbjct: 1 MATLELRNVNKTYGSGLPDTLKNIEIAIDSGEFLILVGPSGCGKSTLMNCIAGLEDISGG 60 Query: 61 AIMIGDQDVSGMSPKDRDIAMVFQSYALYPTMSVRENIEFGLKIRKMPQADIDAEVARVA 120 AI++ QD+SGMSPKDRDIAMVFQSYALYPTM+VR+NI FGLKIRKM ADID EVARVA Sbjct: 61 AILVEGQDISGMSPKDRDIAMVFQSYALYPTMTVRDNIAFGLKIRKMAPADIDTEVARVA 120 Query: 121 KLLQIEHLLNRKPGQLSGGQQQRVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTEMKL 180 KLLQIEHLL RKPGQLSGGQQQRVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTE+KL Sbjct: 121 KLLQIEHLLARKPGQLSGGQQQRVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTEIKL 180 Query: 181 MHQRLKTTTVYVTHDQIEAMTLGDKVAVMKDGIIQQFGTPKEIYNNPANQFVASFIGSPP 240 MHQRLKTTTVYVTHDQIEAMTLGDKVAVMKDGIIQQFGTP++IYN+PAN FVASFIGSPP Sbjct: 181 MHQRLKTTTVYVTHDQIEAMTLGDKVAVMKDGIIQQFGTPQQIYNDPANLFVASFIGSPP 240 Query: 241 MNFVPLRLQRKDGRLVALLDSGQARCELALNTTEAGLEDRDVILGLRPEQIMLAAGEGDS 300 MNF+PLRLQR+DG+L ALLDSGQARCEL L E GLE R+VILG+RPEQI +A G S Sbjct: 241 MNFIPLRLQRRDGQLWALLDSGQARCELPLGAMEEGLESREVILGIRPEQIGIAQGGDGS 300 Query: 301 ASSIRAEVQVTEPTGPDTLVFVQLNDTKVCCRLAPDVAPQVGETLTLQFDPSKVLLFDAN 360 SIRAEVQVTEPTGPDTLVFV+LN TKVCCRLAPD APQVG +L LQF+PSKVLLFDA Sbjct: 301 LPSIRAEVQVTEPTGPDTLVFVELNQTKVCCRLAPDAAPQVGASLELQFEPSKVLLFDAQ 360 Query: 361 TGERLGTASSLPAQGHADNVAQFKGR 386 +GERLG + Q A VAQ KGR Sbjct: 361 SGERLGLLGAARPQERAAKVAQLKGR 386 Lambda K H 0.318 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 565 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 386 Length of database: 386 Length adjustment: 30 Effective length of query: 356 Effective length of database: 356 Effective search space: 126736 Effective search space used: 126736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory