Align Methylisocitrate lyase (EC 4.1.3.30) (characterized)
to candidate WP_090447773.1 BLS63_RS21130 methylisocitrate lyase
Query= reanno::pseudo6_N2E2:Pf6N2E2_6061 (294 letters) >NCBI__GCF_900100495.1:WP_090447773.1 Length = 298 Score = 531 bits (1369), Expect = e-156 Identities = 271/291 (93%), Positives = 279/291 (95%) Query: 4 STPGQRFRDAVANEHPLQVVGTINANHALLAKRAGFKAIYLSGGGVAAGSLGVPDLGITG 63 STPGQRFRDAVA EHPLQVVG INANHALLA+RAGFKAIYLSGGGVAAGSLG+PDLGITG Sbjct: 8 STPGQRFRDAVAEEHPLQVVGAINANHALLAQRAGFKAIYLSGGGVAAGSLGLPDLGITG 67 Query: 64 LDDVLTDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVKSMIKFGAAAIHIEDQVGAKRC 123 LDDVLTDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVKSMIKFGAAAIHIEDQVGAKRC Sbjct: 68 LDDVLTDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVKSMIKFGAAAIHIEDQVGAKRC 127 Query: 124 GHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALDRAAACIEAGAD 183 GHRPNKEIV+QQEMVDRIKAAVDARTD SFVIMARTDALAVEGL SALDRA ACIEAGAD Sbjct: 128 GHRPNKEIVTQQEMVDRIKAAVDARTDPSFVIMARTDALAVEGLNSALDRAEACIEAGAD 187 Query: 184 MIFPEAITELEMYKLFASRVKAPILANITEFGATPLYTTEQLAGADVSLVLYPLSAFRAM 243 MIFPEAITEL MYK FA R+KAPILANITEFGATPLYTTE+LA DVSLVLYPLSAFRAM Sbjct: 188 MIFPEAITELAMYKTFAERIKAPILANITEFGATPLYTTEELASVDVSLVLYPLSAFRAM 247 Query: 244 NKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK 294 NKAAENVYTA+RRDGTQ+NVIDTMQTRMELYDRI+YH FEQ LDALFAAKK Sbjct: 248 NKAAENVYTALRRDGTQKNVIDTMQTRMELYDRINYHAFEQHLDALFAAKK 298 Lambda K H 0.320 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 349 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 294 Length of database: 298 Length adjustment: 26 Effective length of query: 268 Effective length of database: 272 Effective search space: 72896 Effective search space used: 72896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate WP_090447773.1 BLS63_RS21130 (methylisocitrate lyase)
to HMM TIGR02317 (prpB: methylisocitrate lyase (EC 4.1.3.30))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02317.hmm # target sequence database: /tmp/gapView.24608.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02317 [M=285] Accession: TIGR02317 Description: prpB: methylisocitrate lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-136 440.0 1.4 1.9e-136 439.8 1.4 1.0 1 lcl|NCBI__GCF_900100495.1:WP_090447773.1 BLS63_RS21130 methylisocitrate l Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900100495.1:WP_090447773.1 BLS63_RS21130 methylisocitrate lyase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 439.8 1.4 1.9e-136 1.9e-136 2 284 .. 11 295 .. 10 296 .. 0.99 Alignments for each domain: == domain 1 score: 439.8 bits; conditional E-value: 1.9e-136 TIGR02317 2 gkalrellkkedilqipGainalvallaekaGfeavYlsGaalaa.slglPDlglttleevaeearrit 69 g+++r+++++e++lq++Gaina++alla++aGf+a+YlsG+++aa slglPDlg+t l++v++++rrit lcl|NCBI__GCF_900100495.1:WP_090447773.1 11 GQRFRDAVAEEHPLQVVGAINANHALLAQRAGFKAIYLSGGGVAAgSLGLPDLGITGLDDVLTDVRRIT 79 789****************************************999*********************** PP TIGR02317 70 rvtklpllvDaDtGfGe.alnvartvkeleeagvaavhieDqvapkkCGhldgkelvskeemvkkikaa 137 +v++lpllvD+DtGfG+ a+nvartvk++++ g+aa+hieDqv +k+CGh+++ke+v+++emv++ikaa lcl|NCBI__GCF_900100495.1:WP_090447773.1 80 DVCDLPLLVDVDTGFGSsAFNVARTVKSMIKFGAAAIHIEDQVGAKRCGHRPNKEIVTQQEMVDRIKAA 148 ****************889************************************************** PP TIGR02317 138 vkakkdedfvliaRtDaraveGldaaieRakaYveaGadaiftealeseeefrefakavkvpllanmte 206 v+a++d++fv++aRtDa aveGl++a++Ra+a +eaGad+if+ea+++++++++fa+++k+p+lan+te lcl|NCBI__GCF_900100495.1:WP_090447773.1 149 VDARTDPSFVIMARTDALAVEGLNSALDRAEACIEAGADMIFPEAITELAMYKTFAERIKAPILANITE 217 ********************************************************************* PP TIGR02317 207 fGktplltadeleelgykiviyPvtalRaalkaaekvyeelkkkGtqkelldklqtRkelYellgyedy 275 fG tpl+t++el+++ +++v+yP++a+Ra++kaae+vy+ l+++Gtqk+++d++qtR elY+ ++y+++ lcl|NCBI__GCF_900100495.1:WP_090447773.1 218 FGATPLYTTEELASVDVSLVLYPLSAFRAMNKAAENVYTALRRDGTQKNVIDTMQTRMELYDRINYHAF 286 ********************************************************************* PP TIGR02317 276 ekkdkelfk 284 e++++ lf+ lcl|NCBI__GCF_900100495.1:WP_090447773.1 287 EQHLDALFA 295 ***999985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (285 nodes) Target sequences: 1 (298 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 8.66 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory