Align aconitate hydratase (EC 4.2.1.3) (characterized)
to candidate WP_090447774.1 BLS63_RS21135 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase
Query= BRENDA::P36683 (865 letters) >NCBI__GCF_900100495.1:WP_090447774.1 Length = 866 Score = 1369 bits (3544), Expect = 0.0 Identities = 667/856 (77%), Positives = 753/856 (87%) Query: 1 MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEFLLDLLTNRVPPGVDEA 60 MLE YR HVA RA +G+ P L+A Q A LVELLKNPPAGEEE LLDL+TNRVPPGVDEA Sbjct: 1 MLEAYRNHVAARAEQGLPPLALNAEQTAGLVELLKNPPAGEEEVLLDLITNRVPPGVDEA 60 Query: 61 AYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPLIDALDDAKLAPIAAKALS 120 AYVKAGFL+A+AKG A SPLL+ +A+ELLGTMQGGYNI L++ LDDA+L +AA+ L Sbjct: 61 AYVKAGFLSAVAKGAAGSPLLSKTRAVELLGTMQGGYNIATLVELLDDAELGSVAAEQLK 120 Query: 121 HTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRPALAEKLTVTVFKVTGETNTD 180 HTLLMFD F+DV EKAKAGN +AK V+QSWAD EWF RPA+AEK +++VFKVTGETNTD Sbjct: 121 HTLLMFDAFHDVAEKAKAGNAHAKAVLQSWADGEWFTKRPAIAEKYSLSVFKVTGETNTD 180 Query: 181 DLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVGPIKQIEALQQKGFPLAYVGDVV 240 DLSPAPDAWSRPDIPLHALAMLK AR+GIEP+Q G +GP+KQI A++ KGFP+AYVGDVV Sbjct: 181 DLSPAPDAWSRPDIPLHALAMLKMARDGIEPEQQGAIGPLKQIAAIKAKGFPVAYVGDVV 240 Query: 241 GTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGGKIAPIFFNTMEDAGALPIEVDVSN 300 GTGSSRKSATNSVLWF GDDIP+VPNKR GG C G KIAPIF+NTMEDAGALPIE DV+N Sbjct: 241 GTGSSRKSATNSVLWFFGDDIPYVPNKRAGGFCFGNKIAPIFYNTMEDAGALPIEFDVAN 300 Query: 301 LNMGDVIDVYPYKGEVRNHETGELLATFELKTDVLIDEVRAGGRIPLIIGRGLTTKAREA 360 L MGDVIDVYPY G+V H + +++ FELKT+VL+DEVRAGGRIPLI+GRGLT KAR Sbjct: 301 LEMGDVIDVYPYAGKVCKHGSDQVITRFELKTEVLLDEVRAGGRIPLIVGRGLTEKARAE 360 Query: 361 LGLPHSDVFRQAKDVAESDRGFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGPM 420 LGL S++F + A+S +GF+LAQKMVG+ACGV G+RPG YCEPKMT+VGSQDTTGPM Sbjct: 361 LGLGVSELFNKPVAPADSGKGFTLAQKMVGKACGVTGVRPGTYCEPKMTTVGSQDTTGPM 420 Query: 421 TRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH 480 TRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDF+ +RGGVSL+PGDG+IH Sbjct: 421 TRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDFMRSRGGVSLKPGDGIIH 480 Query: 481 SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM 540 SWLNRMLLPDTVGTGGDSHTRFP+GISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGK+ Sbjct: 481 SWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKL 540 Query: 541 QPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASA 600 QPGITLRDLVHAIP YAI++GLLTVEKKGK N FSGRILEIEGL +L VEQAFEL+DASA Sbjct: 541 QPGITLRDLVHAIPYYAIQKGLLTVEKKGKINAFSGRILEIEGLDELTVEQAFELSDASA 600 Query: 601 ERSAAGCTIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEA 660 ERSAAGCTIKL ++ I EYL SNI LL+WMI EGYGD RTLERR Q ME WLANP+L+ A Sbjct: 601 ERSAAGCTIKLPEKAIAEYLQSNITLLRWMIGEGYGDARTLERRAQAMEAWLANPQLMSA 660 Query: 661 DADAEYAAVIDIDLADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAA 720 DADAEYA V++IDLA+I +PILCAPNDPDDAR LS+VQGEKIDEVFIGSCMTNIGHFRAA Sbjct: 661 DADAEYAEVVEIDLAEINQPILCAPNDPDDARLLSSVQGEKIDEVFIGSCMTNIGHFRAA 720 Query: 721 GKLLDAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVA 780 GKLLD KG +PTRLW++PPT+MDA QLTEEGYY ++GK+GAR+E+PGCSLCMGNQARVA Sbjct: 721 GKLLDKVKGGIPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVA 780 Query: 781 DGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDT 840 ATVVSTSTRNFPNRLG GANV+LASAELAAVAA+ GKLPT EEY Y +D A D Sbjct: 781 ANATVVSTSTRNFPNRLGDGANVYLASAELAAVAAITGKLPTVEEYMAYAKDIDSMAADV 840 Query: 841 YRYLNFNQLSQYTEKA 856 YRYL+F+Q++++ E A Sbjct: 841 YRYLSFDQIAEFREAA 856 Lambda K H 0.317 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2127 Number of extensions: 76 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 865 Length of database: 866 Length adjustment: 42 Effective length of query: 823 Effective length of database: 824 Effective search space: 678152 Effective search space used: 678152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory