Align 3-Hydroxy-3-isohexenylglutaryl-CoA lyase (EC 4.1.3.26) (characterized)
to candidate WP_090448390.1 BLS63_RS24350 hydroxymethylglutaryl-CoA lyase
Query= BRENDA::Q9I2A0 (300 letters) >NCBI__GCF_900100495.1:WP_090448390.1 Length = 302 Score = 488 bits (1255), Expect = e-143 Identities = 239/300 (79%), Positives = 270/300 (90%) Query: 1 MNLPKKVRLVEVGPRDGLQNEKQPIEVADKIRLVDDLSAAGLDYIEVGSFVSPKWVPQMA 60 M+LP++VRLVEVGPRDGLQNEKQPI VADK+RLVD+LSAAGL YIEVGSFVSPKWVPQMA Sbjct: 1 MSLPQQVRLVEVGPRDGLQNEKQPIGVADKVRLVDELSAAGLRYIEVGSFVSPKWVPQMA 60 Query: 61 GSAEVFAGIRQRPGVTYAALAPNLKGFEAALESGVKEVAVFAAASEAFSQRNINCSIKDS 120 GS EVFA I++ PGVTYAAL PNLKGFEAALE+GVKEVAVFAAASEAFSQ+NINCSI DS Sbjct: 61 GSREVFAQIQRLPGVTYAALTPNLKGFEAALEAGVKEVAVFAAASEAFSQKNINCSIADS 120 Query: 121 LERFVPVLEAARQHQVRVRGYISCVLGCPYDGDVDPRQVAWVARELQQMGCYEVSLGDTI 180 L+RF+P++EAA QH VRVRGY+SCVLGCPY+G+V +VAWVA+EL MGCYE+SLGDTI Sbjct: 121 LQRFLPLMEAASQHGVRVRGYVSCVLGCPYEGEVSAERVAWVAKELYAMGCYEISLGDTI 180 Query: 181 GVGTAGATRRLIEAVASEVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSSVAGLGGC 240 GVGTAG TR++ + V ++PR++LAGHFHDTYGQALANIYASLLEGI VFDSSVAGLGGC Sbjct: 181 GVGTAGKTRQMFDVVGRDIPRDKLAGHFHDTYGQALANIYASLLEGIGVFDSSVAGLGGC 240 Query: 241 PYAKGATGNVASEDVLYLLNGLEIHTGVDMHALVDAGQRICAVLGKSNGSRAAKALLAKA 300 PYAKGATGNVA+EDVLYLLNGL I TG+D+ L+ AGQRIC VLGK NGSR A+ALL A Sbjct: 241 PYAKGATGNVATEDVLYLLNGLGIGTGIDLDQLIAAGQRICTVLGKENGSRVARALLQVA 300 Lambda K H 0.318 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 380 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 300 Length of database: 302 Length adjustment: 27 Effective length of query: 273 Effective length of database: 275 Effective search space: 75075 Effective search space used: 75075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory