Align ABC transporter for L-Arginine and L-Citrulline, permease component 2 (characterized)
to candidate WP_090448709.1 BLS63_RS26100 ABC transporter
Query= reanno::pseudo3_N2E3:AO353_03050 (229 letters) >NCBI__GCF_900100495.1:WP_090448709.1 Length = 229 Score = 383 bits (984), Expect = e-111 Identities = 188/229 (82%), Positives = 213/229 (93%) Query: 1 MLKGYGAVILDGAWLTLQLALSSMALAIVLGLIGVALRLSPIRWLARLGDLYSTVIRGIP 60 ML GYG+ IL+GAWLTL LALSS+A+AI+LGL+G A RLSP++WLA LG+ Y+TVIRGIP Sbjct: 1 MLNGYGSTILEGAWLTLMLALSSVAVAILLGLLGAAFRLSPVKWLAALGETYATVIRGIP 60 Query: 61 DLVLILLIFYGGQDLLNRVAPLLGYDDYIDLNPLVAGIGTLGFIFGAYLSETFRGAFMAI 120 DLVLILLIFYGGQD++NRVAP+LGYD+YID+NP +AG+ T+GFIFGAYLSETFRGAFMAI Sbjct: 61 DLVLILLIFYGGQDMVNRVAPMLGYDEYIDINPFIAGVFTMGFIFGAYLSETFRGAFMAI 120 Query: 121 PKGQAEAGAAYGMSSFQVFFRVLVPQMIRLAIPGFTNNWLVLTKATALISVVGLQDMMFK 180 PKGQAEAG AYGMS +VFFR+LVPQMIR AIPGFTNNWLVLTKATALISVVGLQDMMFK Sbjct: 121 PKGQAEAGLAYGMSGAKVFFRILVPQMIRFAIPGFTNNWLVLTKATALISVVGLQDMMFK 180 Query: 181 AKQAADATREPFTFFLAVAAMYLVITSVSLLALRHLEKRYSVGVRAADL 229 AK AADATREPFTF+LAVAA+YLV+TSVSLLALR L+KRYSVG +AADL Sbjct: 181 AKSAADATREPFTFYLAVAALYLVLTSVSLLALRFLDKRYSVGTKAADL 229 Lambda K H 0.329 0.144 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 288 Number of extensions: 9 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 229 Length of database: 229 Length adjustment: 23 Effective length of query: 206 Effective length of database: 206 Effective search space: 42436 Effective search space used: 42436 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory