Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_092349201.1 BLU87_RS12690 ABC transporter ATP-binding protein
Query= uniprot:A0A165KC86 (260 letters) >NCBI__GCF_900107645.1:WP_092349201.1 Length = 258 Score = 234 bits (598), Expect = 1e-66 Identities = 117/252 (46%), Positives = 171/252 (67%), Gaps = 2/252 (0%) Query: 6 NEVVLKVAGISKRFGGLQALSDVGITIKRGQVYGLIGPNGAGKTTFFNVITGLYTPDAGT 65 NE++L ++KRFGGL A+ D+ + +K GQ+YG+IGPNGAGKTT FN ITG+Y + G+ Sbjct: 4 NELIL--TNVTKRFGGLTAVDDLSMKVKEGQIYGVIGPNGAGKTTVFNCITGIYKSEEGS 61 Query: 66 FELAGKPYEPTAVHEVAKAGIARTFQNIRLFAEMTALENVMVGRHIRTGSGLFGAVFRTK 125 G+ +++ GI RTFQ IRLF++M+ EN+M GRHI++ ++ + T Sbjct: 62 VNWNGEEIRGMTPYQIVDRGIVRTFQTIRLFSQMSVAENIMSGRHIKSKQKMWHGIVHTP 121 Query: 126 GFKAEEAAIAKRAQELLDYVGIGKFADYKARTLSYGDQRRLEIARALATDPQLIALDEPA 185 + +E + +E L++ + +FA ++L YG QRR+E+ARA+A +P L+ LDEPA Sbjct: 122 ASRRDELDNWCKVEEQLEFFQLSEFAATPTKSLPYGIQRRVEMARAMAVEPTLLILDEPA 181 Query: 186 AGMNATEKVQLRELIDRIRNDNRTILLIEHDVKLVMGLCDRVTVLDYGKQIAEGNPAEVQ 245 AG+N E + L E I +IR T+LLIEHD+ +VM + D +TV+++GK+IAEG PAEVQ Sbjct: 182 AGLNDKETLGLLETIYKIREKGVTVLLIEHDMDMVMEVTDYLTVINFGKKIAEGTPAEVQ 241 Query: 246 KNEKVIEAYLGT 257 KN VIEAYLG+ Sbjct: 242 KNPAVIEAYLGS 253 Lambda K H 0.319 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 193 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 260 Length of database: 258 Length adjustment: 24 Effective length of query: 236 Effective length of database: 234 Effective search space: 55224 Effective search space used: 55224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory