Align Methylmalonyl-CoA epimerase; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 (characterized)
to candidate WP_092483262.1 BM299_RS09150 methylmalonyl-CoA epimerase
Query= SwissProt::O58010 (136 letters) >NCBI__GCF_900115975.1:WP_092483262.1 Length = 133 Score = 161 bits (407), Expect = 4e-45 Identities = 80/132 (60%), Positives = 101/132 (76%), Gaps = 1/132 (0%) Query: 4 MFKRIDHVGIAVKNLEEAIKIWEG-LGFKVEEIEEVPDQKVKVAVIKVGENRIELLEATT 62 M K+IDH+GIAVK++ A +E LG KV E E V +QKVKVA I G++ +ELLE+TT Sbjct: 1 MIKKIDHIGIAVKDMAAAKAFYENVLGLKVTEEEVVEEQKVKVAFIPTGDSEVELLESTT 60 Query: 63 EDSPIAKFIEKRGEGIHHLAIRVENIESKLEELKQKGYKLIDEKPRVGAGGAKIAFIHPK 122 D PIA++IEK GEGI H+A RV+N+E +L +LK G +LIDEKPR GAGGAKIAF+HPK Sbjct: 61 PDGPIARYIEKNGEGIQHIAFRVDNLEERLAQLKAAGVRLIDEKPRRGAGGAKIAFLHPK 120 Query: 123 SVTGVLLELCER 134 + G L+ELCER Sbjct: 121 ATFGTLVELCER 132 Lambda K H 0.317 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 102 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 136 Length of database: 133 Length adjustment: 15 Effective length of query: 121 Effective length of database: 118 Effective search space: 14278 Effective search space used: 14278 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 42 (20.8 bits)
Align candidate WP_092483262.1 BM299_RS09150 (methylmalonyl-CoA epimerase)
to HMM TIGR03081 (mce: methylmalonyl-CoA epimerase (EC 5.1.99.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03081.hmm # target sequence database: /tmp/gapView.7394.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03081 [M=129] Accession: TIGR03081 Description: metmalonyl_epim: methylmalonyl-CoA epimerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-52 163.7 0.2 1.6e-52 163.6 0.2 1.0 1 lcl|NCBI__GCF_900115975.1:WP_092483262.1 BM299_RS09150 methylmalonyl-CoA Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900115975.1:WP_092483262.1 BM299_RS09150 methylmalonyl-CoA epimerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 163.6 0.2 1.6e-52 1.6e-52 1 129 [] 4 131 .. 4 131 .. 0.99 Alignments for each domain: == domain 1 score: 163.6 bits; conditional E-value: 1.6e-52 TIGR03081 1 kldhvaiavkdleeaaklyrdvlGakvseeeelpeqgvkvvflelgetklellepleedspiakflekk 69 k+dh++iavkd+ +a+++y++vlG+kv+eee ++eq+vkv+f+ g++++elle+++ d+pia+++ek+ lcl|NCBI__GCF_900115975.1:WP_092483262.1 4 KIDHIGIAVKDMAAAKAFYENVLGLKVTEEEVVEEQKVKVAFIPTGDSEVELLESTTPDGPIARYIEKN 72 79******************************************************************* PP TIGR03081 70 kgeGlhhialevddieaaletlkekgvrlldeepriGahGkkvaFlhPkdtgGvLielee 129 geG+ hia++vd++e+ l++lk +gvrl+de+pr Ga+G+k+aFlhPk t G L+el+e lcl|NCBI__GCF_900115975.1:WP_092483262.1 73 -GEGIQHIAFRVDNLEERLAQLKAAGVRLIDEKPRRGAGGAKIAFLHPKATFGTLVELCE 131 .*********************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (129 nodes) Target sequences: 1 (133 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 5.36 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory