GapMind for Amino acid biosynthesis

 

Alignments for a candidate for dapF in Sulfurihydrogenibium azorense Az-Fu1

Align Diaminopimelate epimerase; DAP epimerase; EC 5.1.1.7; PLP-independent amino acid racemase (uncharacterized)
to candidate WP_228357427.1 SULAZ_RS04740 diaminopimelate epimerase

Query= curated2:A8MH77
         (280 letters)



>NCBI__GCF_000021545.1:WP_228357427.1
          Length = 292

 Score =  224 bits (571), Expect = 2e-63
 Identities = 111/274 (40%), Positives = 171/274 (62%), Gaps = 5/274 (1%)

Query: 5   FKKMQGTGNDFIVVKYDDFPFEEKLSQLAEKICDRHFGIGADGLLIVNPSSIADIRMDYY 64
           F K  G GND+IV+  ++  FE       +KICD H+GIG+DG+L+  PSS+AD ++  +
Sbjct: 10  FVKSHGLGNDYIVIDSENITFEIT-QDFIKKICDVHYGIGSDGILVKYPSSVADFKLRIF 68

Query: 65  NSDGSIAAMCGNGIRCFSKFVFDEGFLRTKQFSVETLDGIKEIAIIEEK---GTVKSVEV 121
           N DGS A   GNG+R F KF++D G+   ++F+VET  G+   A IEEK   G  K + V
Sbjct: 69  NPDGSEAEKSGNGLRIFCKFLYDYGYTNKEEFTVETKGGLVR-AKIEEKNKFGKAKVITV 127

Query: 122 NMGQVTYDTEKIPVVSEDGYFINKKITVGGQDFIITAVSMGVPHVIIFTEKLDLEQIKFF 181
           +MG+  ++  KIPV ++   F+ +K+ VG +++ +  VS+G PH +I  ++L+ E+IK +
Sbjct: 128 DMGKAIFEASKIPVKTDKKEFLGEKVKVGDKEYEVNCVSVGNPHCVIIKKELNEEEIKTY 187

Query: 182 GPLIEKHAIFPKKTNVNFVHRIDKDNIAVRTWERGAGYTLACGTGSTSAVAVANKLGLVN 241
           G LIE H +FP + NV FV  I +D   +  WERGAG+T A G+ S +  +V  K GL  
Sbjct: 188 GSLIENHPLFPNRINVQFVKPISEDQAEILIWERGAGFTYASGSSSCAVASVLVKKGLAK 247

Query: 242 NNVNVEVEGGNIKIKIKESGNLFMEGPAENICSG 275
           N++ +++ GG +KI + +  N+ M G  + IC G
Sbjct: 248 NDITIKMIGGELKITVDKDWNIRMTGEVQEICRG 281


Lambda     K      H
   0.321    0.140    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 221
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 280
Length of database: 292
Length adjustment: 26
Effective length of query: 254
Effective length of database: 266
Effective search space:    67564
Effective search space used:    67564
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate WP_228357427.1 SULAZ_RS04740 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

Software error:

../bin/blast/fastacmd -i /tmp/list.5850.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.5850.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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