Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate WP_254914254.1 BZY95_RS19345 ABC transporter ATP-binding protein
Query= uniprot:Q1MCU3 (247 letters) >NCBI__GCF_002151265.1:WP_254914254.1 Length = 253 Score = 158 bits (399), Expect = 1e-43 Identities = 90/233 (38%), Positives = 134/233 (57%), Gaps = 1/233 (0%) Query: 10 PLLQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVV 69 PL+ G+ TYYG L GVD+ + GE +SL+G NG GK+T + +I G R G V Sbjct: 19 PLIDARGLHTYYGESHVLHGVDLRLMPGETLSLMGRNGMGKTTTLKSILGFVPPREGEVS 78 Query: 70 FEGRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLDNLKHFAEDVEKIFTL 129 +G+ R ++ R I PEGR IFP ++V E+L M A + +E++ Sbjct: 79 IKGKSTARRRPWQLIRQGIGYVPEGRGIFPGISVREHLIMAARPNERGESPWTLERVLDT 138 Query: 130 FPRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRKL 189 FPRL +R G LSGGEQQMLSIGRAL P +++LDE + GLAPLI I++ IR++ Sbjct: 139 FPRLGQRINHDGSFLSGGEQQMLSIGRALTTNPDMVILDEATEGLAPLIRDEIWKIIREI 198 Query: 190 NEAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKELLANPEVRAAYL 242 +A G+ +V++N L ++ + ++V G+V SG + L +P + +L Sbjct: 199 -KASGIASIIVDKNIDNLLEVADKHLLLVKGEVVYSGDSQGLREDPSILDTHL 250 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 168 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 253 Length adjustment: 24 Effective length of query: 223 Effective length of database: 229 Effective search space: 51067 Effective search space used: 51067 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory