Align L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (characterized)
to candidate YP_001325787.1 Smed_0092 bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase
Query= reanno::azobra:AZOBR_RS23695 (1235 letters) >NCBI__GCF_000017145.1:YP_001325787.1 Length = 1233 Score = 1620 bits (4194), Expect = 0.0 Identities = 853/1227 (69%), Positives = 961/1227 (78%), Gaps = 9/1227 (0%) Query: 10 AAPGEAAPFADFAPPIRPATELRAAITAAYRRPEPECLPFLFEQASLPPGVITAAAATAR 69 AAP APFADFAPP++P + LR AITAAYRRPE ECLP L E A+ AA+ TAR Sbjct: 13 AAP---APFADFAPPVQPQSTLRQAITAAYRRPETECLPPLVEAATQSKETREAASRTAR 69 Query: 70 KLITALRAKPRGRGVEGLIHEYSLSSQEGMALMCLAEALLRIPDHATRDALIRDKIAGGD 129 KLI ALR K G GVEGL+ EYSLSSQEG+ALMCLAEALLRIPD ATRDALIRDKIA G+ Sbjct: 70 KLIEALRGKHSGSGVEGLVQEYSLSSQEGVALMCLAEALLRIPDTATRDALIRDKIADGN 129 Query: 130 WQAHLGKGGSMFVNAATWGLLITGKLTSAGGEQALSSALTRLIARGGEPLIRRGVDFAMR 189 W++HLG S+FVNAATWGL++TGKLTS +++LS+ALTRLI+R GEP+IRRGVD AMR Sbjct: 130 WKSHLGGSRSLFVNAATWGLVVTGKLTSTVNDRSLSAALTRLISRCGEPVIRRGVDMAMR 189 Query: 190 MMGEQFVTGQTIQEALTNARTMEAEGFRYSYDMLGEAALTAEDAARYYADYVNAIHAIGT 249 MMGEQFVTG+TI+EAL ++ +E +GFRYSYDMLGEAA TA DA RYY DY +AIHAIG Sbjct: 190 MMGEQFVTGETIEEALKRSKELEGKGFRYSYDMLGEAATTAADAERYYRDYESAIHAIGK 249 Query: 250 ASAGRGVYEGPGISIKLSAIHPRYSRAQADRVMDELLPRVKALALLAKGYDIGLNIDAEE 309 ASAGRG+YEGPGISIKLSA+HPRY+RAQA RVM ELLP+VKALALLA+ YDIGLNIDAEE Sbjct: 250 ASAGRGIYEGPGISIKLSALHPRYTRAQAVRVMGELLPKVKALALLARKYDIGLNIDAEE 309 Query: 310 ADRLELSLDLMESLCFDPDLAGWNGIGFVVQAYGKRCPYVIDFLIDLARRSGHRLMIRLV 369 ADRLELSLDL+E LC D +L+GWNG+GFVVQAYGKRCP+V+DF+IDLARRSG R+M+RLV Sbjct: 310 ADRLELSLDLLEELCLDAELSGWNGMGFVVQAYGKRCPFVLDFVIDLARRSGRRIMVRLV 369 Query: 370 KGAYWDSEIKRAQLDGLPDFPVYTRKVYTDVSYVACARKLLAAPEAVFPQFATHNAQTLA 429 KGAYWD+EIKRAQLDGL DFPV+TRK++TDVSY+ACA KLLAA + +FPQFATHNAQTLA Sbjct: 370 KGAYWDAEIKRAQLDGLEDFPVFTRKIHTDVSYLACAAKLLAATDVIFPQFATHNAQTLA 429 Query: 430 TIYEMAGSDFQVGKYEFQCLHGMGEPLYKEVV--GPLKRPCRIYAPVGTHETLLAYLVRR 487 IY MAG DFQVGKYEFQCLHGMGEPLY+EVV G L RPCRIYAPVGTHETLLAYLVRR Sbjct: 430 AIYHMAGKDFQVGKYEFQCLHGMGEPLYEEVVGLGNLDRPCRIYAPVGTHETLLAYLVRR 489 Query: 488 LLENGANSSFVNRIADPAVPVDELVADPVAVARAIAPTGAPHALIALPRNLYAPERANSA 547 LLENGANSSFV+RI DP V +DEL+ADPV + R + GA H IALP L+ R NSA Sbjct: 490 LLENGANSSFVHRINDPKVSIDELIADPVEIVRGMPVVGARHDKIALPAELFGDSRTNSA 549 Query: 548 GIDLSDETELARLSAALSASAEMTWTAAPLLADGERAGQAQPVRNPADRRDVVGSVTEAS 607 G+DLS+E LA L+ AL ASA WT P LA G AG+ PV NP D RDVVGSVTE S Sbjct: 550 GLDLSNEETLASLTDALKASAANGWTCVPRLATGPVAGETLPVLNPGDHRDVVGSVTETS 609 Query: 608 EALVAEAFGHAVAAASAWAATPPEERAASLFRAADTMQERMPTLLGLIVREAGKSLPNAI 667 E A A AA+ WAA P ERAA L RAA MQ RMPTLLGLI+REAGKS NAI Sbjct: 610 EEDARRAARLAAEAAADWAAVSPSERAACLDRAAGLMQARMPTLLGLIIREAGKSALNAI 669 Query: 668 AEVREAIDFLRYYGAQVRDRFDNATHRPLGPVVCISPWNFPLAIFSGQIAAALAAGNPVL 727 AEVREAIDFLRYY Q R R HRPLGPVVCISPWNFPLAIF+GQIAAAL AGNPVL Sbjct: 670 AEVREAIDFLRYYAEQTR-RTLGPGHRPLGPVVCISPWNFPLAIFTGQIAAALVAGNPVL 728 Query: 728 AKPAEETPLIAAEAVRILHAAGIPAGALQLLPGAGEVGAALVGHEAVRGVMFTGSTEVAR 787 AKPAEETPLIAAE VRIL AGIPA ALQLLPG G +GAALV GVMFTGSTEVAR Sbjct: 729 AKPAEETPLIAAEGVRILQEAGIPARALQLLPGDGRIGAALVAAPETAGVMFTGSTEVAR 788 Query: 788 LIQRQLAGRLLPDGAPIPLIAETGGQNAMIVDSSALAEQVVGDVIASAFDSAGQRCSALR 847 LIQ QLA RL G PIPLIAETGGQNAMIVDSSALAEQVVGDVIASAFDSAGQRCSALR Sbjct: 789 LIQAQLADRLSLAGRPIPLIAETGGQNAMIVDSSALAEQVVGDVIASAFDSAGQRCSALR 848 Query: 848 ILCLQEDVADRTLAMLKGAMRELRIGNPDRLAVDVGPVISEEARATIAAHIEAMRAKGRN 907 +LCLQEDVA+R L MLKGA+ EL+IG DRL+VDVGPVI+ EA+ I HI MR GR Sbjct: 849 VLCLQEDVAERILTMLKGALHELQIGRTDRLSVDVGPVITSEAKENIEKHIARMRELGRK 908 Query: 908 VEFLPLPAETADGTFIAPTVIEIGGIHELEREVFGPVLHVVRFHRDDLDALVDSINATGY 967 VE + L +ET GTF+ PT+IE+ + +LEREVFGPVLHV+R+ RD LD LVD INATGY Sbjct: 909 VEQIGLASETDQGTFVPPTIIELEKLSDLEREVFGPVLHVIRYRRDGLDRLVDDINATGY 968 Query: 968 GLTFGLHTRIDATIERVTGRIGAGNVYVNRNTIGAVVGVQPFGGHGLSGTGPKAGGPLYL 1027 GLTFGLHTR+D TI VT RI AGN+Y+NRN IGAVVGVQPFGG GLSGTGPKAGGPLYL Sbjct: 969 GLTFGLHTRLDETIAHVTSRIKAGNLYINRNIIGAVVGVQPFGGRGLSGTGPKAGGPLYL 1028 Query: 1028 SRLLSRRPKGWLEFRGPDAARAAGLAYGEWLRAKGFTAEASRCAGYVARSAIGGGAELNG 1087 RL++ P L + +WL KG AEA + SA+G EL G Sbjct: 1029 GRLVATAPVP--PQHSSVHTDPVLLDFTKWLDGKGACAEAEAARNAGSSSALGLDLELPG 1086 Query: 1088 PVGERNLYELHGRGRVLLLPQTRTGLLLQLGAVLATGNSAAVDAPPDLAELLRGLPPALA 1147 PVGERNLY LH RGR+LL+P T +GL QL A LATGNS +DA L L+ LP + Sbjct: 1087 PVGERNLYTLHARGRILLVPATASGLYHQLAAALATGNSVVIDAASGLQPSLKDLPQTVG 1146 Query: 1148 ARVRTTADWRDVGPLAAVLVEGDRERVTAINRRVADLPGPILLVQAATAEALAAGRGEGY 1207 RV + DW GP A LVEGD ER+ A+N+ +A LPGP+LLVQAA++E +A + Y Sbjct: 1147 LRVSWSKDWAADGPFAGALVEGDGERIRAVNKAIAALPGPLLLVQAASSEEIAR-NPDAY 1205 Query: 1208 DLDLLLNERSVSVNTAAAGGNASLVAM 1234 L+ L+ E S S+NTAAAGGNASL+A+ Sbjct: 1206 CLNWLVEEVSASINTAAAGGNASLMAI 1232 Lambda K H 0.319 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 3769 Number of extensions: 166 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 1235 Length of database: 1233 Length adjustment: 48 Effective length of query: 1187 Effective length of database: 1185 Effective search space: 1406595 Effective search space used: 1406595 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 59 (27.3 bits)
Align candidate YP_001325787.1 Smed_0092 (bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase)
to HMM TIGR01238 (delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01238.hmm # target sequence database: /tmp/gapView.4469.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01238 [M=500] Accession: TIGR01238 Description: D1pyr5carbox3: delta-1-pyrroline-5-carboxylate dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4e-224 730.6 1.0 7.5e-224 729.7 0.1 1.7 2 lcl|NCBI__GCF_000017145.1:YP_001325787.1 Smed_0092 bifunctional proline d Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000017145.1:YP_001325787.1 Smed_0092 bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydr # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 729.7 0.1 7.5e-224 7.5e-224 1 497 [. 539 1033 .. 539 1036 .. 0.98 2 ? -1.3 0.1 0.03 0.03 180 196 .. 1115 1131 .. 1110 1138 .. 0.84 Alignments for each domain: == domain 1 score: 729.7 bits; conditional E-value: 7.5e-224 TIGR01238 1 dlygegrknslGvdlaneselksleeqllkaaakkfqaapivgekakaegeaqpvknpadrkdivGq 67 +l+g++r+ns+G+dl+ne++l+sl + l+++aa+ + +p ++ ++ ge+ pv np d++d+vG lcl|NCBI__GCF_000017145.1:YP_001325787.1 539 ELFGDSRTNSAGLDLSNEETLASLTDALKASAANGWTCVPRLATGPV-AGETLPVLNPGDHRDVVGS 604 79****************************************76666.58999************** PP TIGR01238 68 vseadaaevqeavdsavaafaewsatdakeraailerladlleshmpelvallvreaGktlsnaiae 134 v+e++++++++a a +a+a w a+ + eraa+l+r+a l++ +mp+l++l++reaGk+ naiae lcl|NCBI__GCF_000017145.1:YP_001325787.1 605 VTETSEEDARRAARLAAEAAADWAAVSPSERAACLDRAAGLMQARMPTLLGLIIREAGKSALNAIAE 671 ******************************************************************* PP TIGR01238 135 vreavdflryyakqvedvldeesakalGavvcispwnfplaiftGqiaaalaaGntviakpaeqtsl 201 vrea+dflryya+q + +l+ +++lG+vvcispwnfplaiftGqiaaal+aGn v+akpae+t+l lcl|NCBI__GCF_000017145.1:YP_001325787.1 672 VREAIDFLRYYAEQTRRTLGPG-HRPLGPVVCISPWNFPLAIFTGQIAAALVAGNPVLAKPAEETPL 737 ********************98.******************************************** PP TIGR01238 202 iaaravellqeaGvpagviqllpGrGedvGaaltsderiaGviftGstevarlinkalakredap.. 266 iaa++v +lqeaG+pa ++qllpG G +Gaal + + aGv+ftGstevarli+ +la+r lcl|NCBI__GCF_000017145.1:YP_001325787.1 738 IAAEGVRILQEAGIPARALQLLPGDGR-IGAALVAAPETAGVMFTGSTEVARLIQAQLADRLSLAgr 803 **************************9.*********************************875444 PP TIGR01238 267 .vpliaetGGqnamivdstalaeqvvadvlasafdsaGqrcsalrvlcvqedvadrvltlikGamde 332 +pliaetGGqnamivds+alaeqvv dv+asafdsaGqrcsalrvlc+qedva+r+lt++kGa++e lcl|NCBI__GCF_000017145.1:YP_001325787.1 804 pIPLIAETGGQNAMIVDSSALAEQVVGDVIASAFDSAGQRCSALRVLCLQEDVAERILTMLKGALHE 870 4****************************************************************** PP TIGR01238 333 lkvgkpirlttdvGpvidaeakqnllahiekmkakakkvaqvkleddvesekgtfvaptlfelddld 399 l++g+ rl dvGpvi +eak+n+++hi +m++ ++kv q+ l++ e+++gtfv+pt++el++l+ lcl|NCBI__GCF_000017145.1:YP_001325787.1 871 LQIGRTDRLSVDVGPVITSEAKENIEKHIARMRELGRKVEQIGLAS--ETDQGTFVPPTIIELEKLS 935 ********************************************99..999**************** PP TIGR01238 400 elkkevfGpvlhvvrykadeldkvvdkinakGygltlGvhsrieetvrqiekrakvGnvyvnrnlvG 466 +l++evfGpvlhv+ry++d ld++vd ina+Gyglt+G+h+r +et++++++r+k+Gn+y+nrn++G lcl|NCBI__GCF_000017145.1:YP_001325787.1 936 DLEREVFGPVLHVIRYRRDGLDRLVDDINATGYGLTFGLHTRLDETIAHVTSRIKAGNLYINRNIIG 1002 ******************************************************************* PP TIGR01238 467 avvGvqpfGGeGlsGtGpkaGGplylyrltr 497 avvGvqpfGG+GlsGtGpkaGGplyl rl+ lcl|NCBI__GCF_000017145.1:YP_001325787.1 1003 AVVGVQPFGGRGLSGTGPKAGGPLYLGRLVA 1033 ****************************986 PP == domain 2 score: -1.3 bits; conditional E-value: 0.03 TIGR01238 180 qiaaalaaGntviakpa 196 q+aaala+Gn+v+ a lcl|NCBI__GCF_000017145.1:YP_001325787.1 1115 QLAAALATGNSVVIDAA 1131 89**********98766 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (500 nodes) Target sequences: 1 (1233 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 22.35 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory