Alignments for a candidate for mctP in Sinorhizobium fredii NGR234

GapMind for catabolism of small carbon sources

Alignments for a candidate for mctP in Sinorhizobium fredii NGR234

Align Monocarboxylate transport permease protein (characterized)
to candidate YP_002824475.1 NGR_b22770 monocarboxylic acid permease

Query= SwissProt::Q1M7A2
         (491 letters)



>NCBI__GCF_000018545.1:YP_002824475.1
          Length = 502

 Score =  767 bits (1980), Expect = 0.0
 Identities = 378/486 (77%), Positives = 428/486 (88%)

Query: 1   MTTDINGTALAVFIFFFVLVTVMGFVASRWRKPETLAHIDEWGLGGRNFGTWITWFLVGG 60
           MT +I+ +ALAVFIFFFVLVT MGF ASRWR+ E+L H+DEWGLGGR FGTWITWFLVGG
Sbjct: 1   MTANIDMSALAVFIFFFVLVTAMGFFASRWRRAESLDHLDEWGLGGRKFGTWITWFLVGG 60

Query: 61  DFYTAYTVIAVPALVYTVGAYGFFALPYTIVVYPFVFMVMPVLWKRAKDFGYVTAGDVVH 120
           DFYTAYTVIAVPALVY +GAYGFFALPYTI+VYP VF VMP+LWK AK+ GYVTAGDVV 
Sbjct: 61  DFYTAYTVIAVPALVYAIGAYGFFALPYTIIVYPLVFAVMPLLWKAAKERGYVTAGDVVR 120

Query: 121 GQYGSRGLELAVAATGVIATMPYIALQLVGMTAVLKALGLHGELPLAIAFIVLALYTYSA 180
           G YGSR LELAVA+TGVIATMPYIALQL+GM   +KALGL GE+P+ +AF+VLALYTYS+
Sbjct: 121 GTYGSRALELAVASTGVIATMPYIALQLIGMEVAIKALGLTGEMPIVLAFLVLALYTYSS 180

Query: 181 GLRAPALIAFVKDIMIYIVVIAAVALIPSKLGGYANVFASADAAFQAKGSGNLLLGGNQY 240
           GLRAPALIAFVKDIMIYIVV+AA+ ++PSKLGGY+ VF++ADAAF+AKG G +LL   Q 
Sbjct: 181 GLRAPALIAFVKDIMIYIVVLAAIIVVPSKLGGYSAVFSAADAAFEAKGKGGILLDPAQL 240

Query: 241 VAYATLALGSALAAFMYPHTLTGIFASNSGKTIRKNAIMLPAYTLLLGLLALLGYMGHAA 300
           V Y TLALGSALAAFMYPHTLTGIFAS+   TIRKNA++LPAYTLLLGLLALLGYMGHAA
Sbjct: 241 VPYVTLALGSALAAFMYPHTLTGIFASSGANTIRKNAVLLPAYTLLLGLLALLGYMGHAA 300

Query: 301 NLKLDSANDVVPTLFKTLFSGWFSGFAFAAIAIGALVPAAVMSIGAANLFTRNFWKAYVD 360
            L+L S NDVVPTLFKTLF GWF+GFAF+AIAIGALVPAAVMSIGAANLFTRNFWK +V+
Sbjct: 301 GLQLQSNNDVVPTLFKTLFPGWFAGFAFSAIAIGALVPAAVMSIGAANLFTRNFWKVWVN 360

Query: 361 PDVSDAGEAKVAKITSLVVKVGALLVIIFLPTQFALDLQLLGGIWILQTLPALVFGLYTN 420
           PDV+ AGEA+VAKITS++VKVGAL+ I+ LPTQFALDLQLLGG+WILQTLPALVFGLYT+
Sbjct: 361 PDVTAAGEAQVAKITSMLVKVGALVAILLLPTQFALDLQLLGGLWILQTLPALVFGLYTS 420

Query: 421 WFRAPGLLAGWFVGFGGGTFLVWDAGWKPLHLISLGGEPFTVYTGLLALAANIAVAVVVN 480
           WFRAP LLAGW  GF GG+ LVW  G KPLH ++  G   +VYTGLLALA N+ VAVVVN
Sbjct: 421 WFRAPALLAGWAAGFIGGSALVWSDGLKPLHALAFDGVTVSVYTGLLALALNVTVAVVVN 480

Query: 481 ALLPAK 486
            ++PAK
Sbjct: 481 LVVPAK 486


Lambda     K      H
   0.327    0.142    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 911
Number of extensions: 45
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 491
Length of database: 502
Length adjustment: 34
Effective length of query: 457
Effective length of database: 468
Effective search space:   213876
Effective search space used:   213876
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources