Align long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211); acetyl-CoA C-acyltransferase (EC 2.3.1.16) (characterized)
to candidate YP_004140285.1 Mesci_1071 3-hydroxyacyl-CoA dehydrogenase NAD-binding protein
Query= BRENDA::Q64428 (763 letters) >NCBI__GCF_000185905.1:YP_004140285.1 Length = 688 Score = 326 bits (835), Expect = 3e-93 Identities = 226/716 (31%), Positives = 357/716 (49%), Gaps = 47/716 (6%) Query: 47 GDVAVIRINSPNSKVNTLNKEVQSEFVEVMNEIWANDQIRSAVLISSKPGCFVAGADINM 106 GDVAV+ I++P VN L+ V+ ++ + E+ +D +A++I+ FVAGADI Sbjct: 11 GDVAVVTIDNP--PVNALSFHVREPLMQALVEL-RDDASVAAIVIACAGRTFVAGADITE 67 Query: 107 LASCTTPQEAARISQEGQKMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRK 166 E I LE KP VAAI G+ LGGGLELA+ C +R+A K Sbjct: 68 FGKPMQQPELRAI-------VATLETIAKPTVAAIHGTALGGGLELALGCHFRVADAGAK 120 Query: 167 TVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVDPLG 226 LG+PEV LG+LPG GGT RLP++VG A M+++G I A A GLVD + + Sbjct: 121 --LGLPEVKLGLLPGGGGTVRLPRLVGAVKALKMIVSGAPIGATEAHAAGLVDAVFEG-- 176 Query: 227 PGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLMEKLTSYAMTIPFVRQQVYKTV 286 L AVNFA +A + + E L + + Sbjct: 177 ------------DLTTHAVNFAGEIARKGGPFTPVRDRNEGLKQADLA-------AFDVE 217 Query: 287 EEKVKKQTKGLYPAPLKIIDAVKTGLEQGNDAGYLAESEKFGELALTKESKALMGLYNGQ 346 + ++ +GL AP+ +V+ + DA AE F +L + +S+A L+ + Sbjct: 218 AADLARKARGL-EAPIACAQSVRNAVTLPFDAALAAERALFVKLVASDQSRAQRHLFFAE 276 Query: 347 -VLCKKNKFGAPQKTVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVF 405 K ++ + ++ ++GAG MG GIA + G +L +T+ L RG + Sbjct: 277 REAAKLPGKDIVKRRIARVGVIGAGTMGGGIAMAFANGGYSVILLETSHEALQRGLATID 336 Query: 406 KGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLAVKHKVLKEVESV 465 + + V + +L+ + + G DY D+++EAVFED+AVK +V ++E+V Sbjct: 337 RNYSVSVTRGSLSEDAKRQRLAQFKGSTDYVDLADCDLIVEAVFEDMAVKKEVFGKLEAV 396 Query: 466 TPEHCIFASNTSALPINQIAAVSQRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASA 525 I A+NTS L IN+IA + RP+ V+G+H+FSP + M+LLEI+ +KT+ D A++ Sbjct: 397 AKPGAILATNTSYLDINEIAGSTSRPQDVLGLHFFSPANVMKLLEIVRAEKTAPDALATS 456 Query: 526 VAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDALTTGFGFPVGAA 585 V + + GKV +VV GF R LA SE +L EG P ++D + T FG+P+G Sbjct: 457 VDLARRIGKVAVVVGVCHGFVGNRMLAARGSESEALLLEGATPSQIDQVFTDFGWPMGPF 516 Query: 586 TLADEVGIDVAQHVAEDLGKAFGERFGGGSVELLKLMVSKGFLGRKSGKGFYIYQSGSKN 645 + D G+D+ + G + + +G G+K+G+GFY+Y++G++ Sbjct: 517 QMGDLAGLDIGWR---------NRKARGQKAVIADTLCEQGRFGQKTGRGFYLYEAGART 567 Query: 646 KNLNSEIDNILVNLRLP--AKPEVSSDEDIQYRVITRFVNEAVLCLQEGILATPEEGDIG 703 + E++ ++ + P S E+I R + +NE L+EGI A + D+ Sbjct: 568 PVPDPEVEALIRDKAAENGIAPRAISAEEIVERTLYPLINEGAKILEEGIAARASDIDVV 627 Query: 704 AVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQ-FTPCQLLRDLANNSS 758 V G GFP GGP +V L G ++++RL + G F P LL+ +A S Sbjct: 628 WVNGYGFPIGKGGPMFWVGLEGPARIIERLEYWHQRTGKDVFKPAPLLKRMAETGS 683 Lambda K H 0.317 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1059 Number of extensions: 53 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 763 Length of database: 688 Length adjustment: 40 Effective length of query: 723 Effective length of database: 648 Effective search space: 468504 Effective search space used: 468504 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory