Align acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16) (characterized)
to candidate YP_004140539.1 Mesci_1329 acetyl-CoA acetyltransferase
Query= reanno::Phaeo:GFF329 (391 letters) >NCBI__GCF_000185905.1:YP_004140539.1 Length = 392 Score = 605 bits (1560), Expect = e-178 Identities = 301/387 (77%), Positives = 338/387 (87%) Query: 3 NVVIASAARTAVGSFGGAFAKTPAHDLGAAVLQAVVERAGIDKSEVSETILGQVLTAAQG 62 ++VIASAART VGSF GAFA TPAH+LGA V++ ++ RAG++ EV E ILGQVLTAAQG Sbjct: 4 SIVIASAARTPVGSFNGAFAATPAHELGAVVIRELLSRAGVEPGEVDEVILGQVLTAAQG 63 Query: 63 QNPARQAHINAGLPQESAAWSLNQVCGSGLRAVALAAQHIQLGDAAIVCAGGQENMTLSP 122 QNPARQA INAGLP+E+ AW LNQVCGSGLRA+AL Q I +GDA ++ AGGQE+M+LS Sbjct: 64 QNPARQASINAGLPKETTAWGLNQVCGSGLRAIALGMQQIAIGDARVIIAGGQESMSLST 123 Query: 123 HAANLRAGHKMGDMSYIDTMIRDGLWDAFNGYHMGQTAENVAEKWQISREMQDEFAVASQ 182 HA +LRAG KMGD IDTMI+DGLWDAFNGYHMG TAENVA ++QI+RE QD+FA+ASQ Sbjct: 124 HAQHLRAGVKMGDFKLIDTMIKDGLWDAFNGYHMGNTAENVARQFQITREDQDQFALASQ 183 Query: 183 NKAEAAQKAGKFADEIAAFTVKTRKGDIIVDQDEYIRHGATIEAMQKLRPAFAKDGSVTA 242 NKAEAAQKAGKF DEI A T+K +KGD IVDQDEYIRHGATI+AM KL+PAF KDG+VTA Sbjct: 184 NKAEAAQKAGKFKDEIVAVTIKGKKGDTIVDQDEYIRHGATIDAMTKLKPAFDKDGTVTA 243 Query: 243 ANASGLNDGAAATLLMSADDAEKRGIEPLARIASYATAGLDPSIMGVGPIYASRKALEKA 302 ANASG+NDGAA LLMS +A +RGI PLARI S+ATAG+DP IMG GPI ASRKALEKA Sbjct: 244 ANASGINDGAAGALLMSEAEAVRRGITPLARIVSWATAGVDPQIMGTGPIPASRKALEKA 303 Query: 303 GWSVDDLDLVEANEAFAAQACAVNKDMGWDPAIVNVNGGAIAIGHPIGASGCRVLNTLLF 362 GWSV DLDLVEANEAFAAQACAVNKDMGWDP+IVNVNGGAIAIGHPIGASG RV NTL+F Sbjct: 304 GWSVGDLDLVEANEAFAAQACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGARVFNTLVF 363 Query: 363 EMKRRDAKKGLATLCIGGGMGVAMCVE 389 EM+RR AKKGLATLCIGGGMGVAMCVE Sbjct: 364 EMRRRGAKKGLATLCIGGGMGVAMCVE 390 Lambda K H 0.316 0.130 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 522 Number of extensions: 8 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 391 Length of database: 392 Length adjustment: 31 Effective length of query: 360 Effective length of database: 361 Effective search space: 129960 Effective search space used: 129960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate YP_004140539.1 Mesci_1329 (acetyl-CoA acetyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.26126.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-145 470.3 9.4 2.6e-145 470.1 9.4 1.0 1 lcl|NCBI__GCF_000185905.1:YP_004140539.1 Mesci_1329 acetyl-CoA acetyltran Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000185905.1:YP_004140539.1 Mesci_1329 acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 470.1 9.4 2.6e-145 2.6e-145 1 385 [] 7 390 .. 7 390 .. 0.98 Alignments for each domain: == domain 1 score: 470.1 bits; conditional E-value: 2.6e-145 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaag 69 i +a+Rtp+g+++g+++ ++a++L+a vi+ell rag++p ++devilG+vl+a++++n+aR+a ++ag lcl|NCBI__GCF_000185905.1:YP_004140539.1 7 IASAARTPVGSFNGAFAATPAHELGAVVIRELLSRAGVEPGEVDEVILGQVLTAAQGQNPARQASINAG 75 789****************************************************************** PP TIGR01930 70 lpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakled 138 lp+++ a+ +n+vC+Sgl+A+al++q+i+ G+a+v++aGG EsmS + + + r+++k+g++kl d lcl|NCBI__GCF_000185905.1:YP_004140539.1 76 LPKETTAWGLNQVCGSGLRAIALGMQQIAIGDARVIIAGGQESMSLSTHAQH---LRAGVKMGDFKLID 141 *********************************************9975444...479*********** PP TIGR01930 139 qllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..k 202 +++kd+ + ++++mg+tAen+a++++i+Re+qD++al+S++ka++A+++gkfkdeiv v++kgk + lcl|NCBI__GCF_000185905.1:YP_004140539.1 142 TMIKDGlwdAFNGYHMGNTAENVARQFQITREDQDQFALASQNKAEAAQKAGKFKDEIVAVTIKGKkgD 210 *********99********************************************************99 PP TIGR01930 203 kvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivs 271 ++v++De ir + t++++ kLkpaf++ +g tvtA+N+s++nDGAa +llmse+ a + g+tplarivs lcl|NCBI__GCF_000185905.1:YP_004140539.1 211 TIVDQDEYIRHGATIDAMTKLKPAFDK-DG-TVTAANASGINDGAAGALLMSEAEAVRRGITPLARIVS 277 9************************95.9*.6************************************* PP TIGR01930 272 aavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAiA 340 +a+agvdp++mg+gp+pA++kaL+kag+s+ d+dlvE nEAFAaq+ av+k++g d++ vNvnGGAiA lcl|NCBI__GCF_000185905.1:YP_004140539.1 278 WATAGVDPQIMGTGPIPASRKALEKAGWSVGDLDLVEANEAFAAQACAVNKDMG-WDPSIVNVNGGAIA 345 ******************************************************.88************ PP TIGR01930 341 lGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 +GHP+GasGar+ tl+ e+++rg+k GlatlC+ggG+G+A+ +e lcl|NCBI__GCF_000185905.1:YP_004140539.1 346 IGHPIGASGARVFNTLVFEMRRRGAKKGLATLCIGGGMGVAMCVE 390 ******************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (392 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 8.37 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory