Align acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16) (characterized)
to candidate YP_426468.1 Rru_A1380 acetyl-CoA acetyltransferase
Query= reanno::Phaeo:GFF329 (391 letters) >NCBI__GCF_000013085.1:YP_426468.1 Length = 391 Score = 546 bits (1406), Expect = e-160 Identities = 271/390 (69%), Positives = 320/390 (82%) Query: 1 MTNVVIASAARTAVGSFGGAFAKTPAHDLGAAVLQAVVERAGIDKSEVSETILGQVLTAA 60 M +VVIA AARTA+G+F GAF PAH LG ++ + RA ++ +V E +LG VLTA Sbjct: 1 MNDVVIAGAARTAIGAFNGAFGAVPAHTLGEVAIREALNRAKVEAGDVDEVVLGHVLTAG 60 Query: 61 QGQNPARQAHINAGLPQESAAWSLNQVCGSGLRAVALAAQHIQLGDAAIVCAGGQENMTL 120 QGQNPARQA +NAG+P E A+ +NQ+CGSGLR VAL Q I+ GDA IV AGGQE+M++ Sbjct: 61 QGQNPARQAAVNAGIPVERTAYCINQLCGSGLRTVALGFQAIRNGDAKIVVAGGQESMSM 120 Query: 121 SPHAANLRAGHKMGDMSYIDTMIRDGLWDAFNGYHMGQTAENVAEKWQISREMQDEFAVA 180 +PHA++LRAG KMG +DTMI+DGLWDAFNGYHMG TAEN+A+KWQISR QDEFA A Sbjct: 121 APHASHLRAGIKMGPGELVDTMIKDGLWDAFNGYHMGTTAENIADKWQISRAEQDEFACA 180 Query: 181 SQNKAEAAQKAGKFADEIAAFTVKTRKGDIIVDQDEYIRHGATIEAMQKLRPAFAKDGSV 240 SQNKAEAA AGKF DEIAA T+KTRKGD++VD DE+ + G TIE++ K R AF KDG+V Sbjct: 181 SQNKAEAAIAAGKFKDEIAAVTLKTRKGDVVVDTDEHPKAGVTIESLAKARAAFRKDGTV 240 Query: 241 TAANASGLNDGAAATLLMSADDAEKRGIEPLARIASYATAGLDPSIMGVGPIYASRKALE 300 TA NASG+NDGAAA +LMSAD+A RGI PLARI S+ATAG+DP+IMG GPI ASR AL+ Sbjct: 241 TAGNASGINDGAAALVLMSADNARLRGIAPLARIVSWATAGVDPAIMGSGPIPASRLALK 300 Query: 301 KAGWSVDDLDLVEANEAFAAQACAVNKDMGWDPAIVNVNGGAIAIGHPIGASGCRVLNTL 360 KAGW++DDLDL+EANEAFAAQA AVN+DMGWD A VNVNGGAIA+GHPIGASG RVL TL Sbjct: 301 KAGWTIDDLDLIEANEAFAAQAIAVNRDMGWDTAKVNVNGGAIALGHPIGASGARVLVTL 360 Query: 361 LFEMKRRDAKKGLATLCIGGGMGVAMCVER 390 L EM +RDAKKGL TLCIGGGMG+A+CVER Sbjct: 361 LHEMIKRDAKKGLTTLCIGGGMGIALCVER 390 Lambda K H 0.316 0.130 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 490 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 391 Length of database: 391 Length adjustment: 31 Effective length of query: 360 Effective length of database: 360 Effective search space: 129600 Effective search space used: 129600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate YP_426468.1 Rru_A1380 (acetyl-CoA acetyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.7693.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-141 458.0 12.0 1.4e-141 457.8 12.0 1.0 1 lcl|NCBI__GCF_000013085.1:YP_426468.1 Rru_A1380 acetyl-CoA acetyltrans Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000013085.1:YP_426468.1 Rru_A1380 acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 457.8 12.0 1.4e-141 1.4e-141 1 385 [] 6 389 .. 6 389 .. 0.98 Alignments for each domain: == domain 1 score: 457.8 bits; conditional E-value: 1.4e-141 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaaglpe 72 i +a+Rt+ig+++g++ ++a++L++ +i+e+l+ra++++ ++dev+lG+vl+ag+++n+aR+aa++ag+p lcl|NCBI__GCF_000013085.1:YP_426468.1 6 IAGAARTAIGAFNGAFGAVPAHTLGEVAIREALNRAKVEAGDVDEVVLGHVLTAGQGQNPARQAAVNAGIPV 77 7899******************************************************************** PP TIGR01930 73 svpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakledqllkdl 144 + a+ +n++C+Sgl++val+ q+i+ G+a++vvaGG EsmS +p+ ++ r+++k+g +l d+++kd+ lcl|NCBI__GCF_000013085.1:YP_426468.1 78 ERTAYCINQLCGSGLRTVALGFQAIRNGDAKIVVAGGQESMSMAPHASHL---RAGIKMGPGELVDTMIKDG 146 *********************************************86665...69***************** PP TIGR01930 145 ...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..kkvvskDegi 211 + ++++mg+tAen+a+k++isR eqDe+a +S++ka++Ai++gkfkdei v++k++ ++vv++De++ lcl|NCBI__GCF_000013085.1:YP_426468.1 147 lwdAFNGYHMGTTAENIADKWQISRAEQDEFACASQNKAEAAIAAGKFKDEIAAVTLKTRkgDVVVDTDEHP 218 ***99*****************************************************99999********* PP TIGR01930 212 rpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivsaavagvdpeemg 283 ++++t+e+Lak ++af++ +g tvtAgN+s++nDGAaal+lms + a+ g+ plarivs+a+agvdp++mg lcl|NCBI__GCF_000013085.1:YP_426468.1 219 KAGVTIESLAKARAAFRK-DG-TVTAGNASGINDGAAALVLMSADNARLRGIAPLARIVSWATAGVDPAIMG 288 ****************95.9*.6************************************************* PP TIGR01930 284 lgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAiAlGHPlGasGarivlt 355 gp+pA++ aLkkag++i+d+dl+E nEAFAaq++av++++g d++kvNvnGGAiAlGHP+GasGar+++t lcl|NCBI__GCF_000013085.1:YP_426468.1 289 SGPIPASRLALKKAGWTIDDLDLIEANEAFAAQAIAVNRDMG-WDTAKVNVNGGAIALGHPIGASGARVLVT 359 ******************************************.88*************************** PP TIGR01930 356 llkeLkergkkyGlatlCvggGqGaAvile 385 ll+e+ +r++k Gl+tlC+ggG+G+A+ +e lcl|NCBI__GCF_000013085.1:YP_426468.1 360 LLHEMIKRDAKKGLTTLCIGGGMGIALCVE 389 **************************9886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (391 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 8.41 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory