GapMind for catabolism of small carbon sources

 

L-arginine catabolism in Cereibacter sphaeroides ATCC 17029

Best path

bgtB, artP, rocF, odc, puuA, puuB, puuC, puuD, gabT, gabD

Rules

Overview: Arginine utilization in GapMind is based on MetaCyc pathways L-arginine degradation I via arginase (link); II via arginine succinyltransferase (link), III via arginine decarboxylase and agmatinase (link), IV via arginine decarboxylase and agmatine deiminase (link), V via arginine deiminase (link), VI (arginase 2, link), VII (arginase 3, link), VIII via arginase oxidase (link), IX via arginine:pyruvate transaminase (link), X via arginine monooxygenase (link), XIII via proline (link), and XIV via D-ornithine (link). Common intermediates are L-ornithine or L-proline. GapMind does not include pathways XI (link), which is poorly understood, or XII (link), which is not reported in prokaryotes.

71 steps (42 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
bgtB L-arginine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
artP L-arginine ABC transporter, ATPase component ArtP/HisP/AotP/BgtA RSPH17029_RS10165 RSPH17029_RS17510
rocF arginase RSPH17029_RS06535 RSPH17029_RS17500
odc L-ornithine decarboxylase RSPH17029_RS03580
puuA glutamate-putrescine ligase RSPH17029_RS10230 RSPH17029_RS01120
puuB gamma-glutamylputrescine oxidase RSPH17029_RS10235 RSPH17029_RS11565
puuC gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase RSPH17029_RS16005 RSPH17029_RS04350
puuD gamma-glutamyl-gamma-aminobutyrate hydrolase RSPH17029_RS09530 RSPH17029_RS03635
gabT gamma-aminobutyrate transaminase RSPH17029_RS04315 RSPH17029_RS20165
gabD succinate semialdehyde dehydrogenase RSPH17029_RS13480 RSPH17029_RS16005
Alternative steps:
AAP3 L-arginine transporter AAP3
adiA arginine decarboxylase (AdiA/SpeA)
aguA agmatine deiminase
aguB N-carbamoylputrescine hydrolase RSPH17029_RS17110
arcA arginine deiminase
arcB ornithine carbamoyltransferase RSPH17029_RS03670 RSPH17029_RS13430
arcC carbamate kinase
arg-monooxygenase arginine 2-monooxygenase
aroD L-arginine oxidase
artJ L-arginine ABC transporter, periplasmic substrate-binding component ArtJ/HisJ/ArtI/AotJ/ArgT RSPH17029_RS10170 RSPH17029_RS17495
artM L-arginine ABC transporter, permease component 1 (ArtM/HisM/AotM) RSPH17029_RS10180 RSPH17029_RS17505
artQ L-arginine ABC transporter, permease component 2 (ArtQ/HisQ/AotQ) RSPH17029_RS17505 RSPH17029_RS10175
aruF ornithine/arginine N-succinyltransferase subunit AruAI (AruF)
aruG ornithine/arginine N-succinyltransferase subunit AruAII (AruG)
aruH L-arginine:pyruvate transaminase RSPH17029_RS12225 RSPH17029_RS15545
aruI 2-ketoarginine decarboxylase RSPH17029_RS06560
astA arginine N-succinyltransferase
astB N-succinylarginine dihydrolase
astC succinylornithine transaminase RSPH17029_RS03665 RSPH17029_RS04315
astD succinylglutamate semialdehyde dehydrogenase RSPH17029_RS13480 RSPH17029_RS16005
astE succinylglutamate desuccinylase
atoB acetyl-CoA C-acetyltransferase RSPH17029_RS12120 RSPH17029_RS19805
braC ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC
braD ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD) RSPH17029_RS17970 RSPH17029_RS14875
braE ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE) RSPH17029_RS04845 RSPH17029_RS14870
braF ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF) RSPH17029_RS04855 RSPH17029_RS17980
braG ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG) RSPH17029_RS04835 RSPH17029_RS17985
Can1 L-arginine transporter Can1
CAT1 L-arginine transporter CAT1
davD glutarate semialdehyde dehydrogenase RSPH17029_RS13480 RSPH17029_RS04350
davT 5-aminovalerate aminotransferase RSPH17029_RS04315 RSPH17029_RS17535
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase RSPH17029_RS00050 RSPH17029_RS17990
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase RSPH17029_RS16015 RSPH17029_RS04435
gbamidase guanidinobutyramidase RSPH17029_RS17110 RSPH17029_RS15335
gbuA guanidinobutyrase RSPH17029_RS17500 RSPH17029_RS07860
gcdG succinyl-CoA:glutarate CoA-transferase RSPH17029_RS00595
gcdH glutaryl-CoA dehydrogenase RSPH17029_RS14905 RSPH17029_RS05935
glaH glutarate 2-hydroxylase, succinate-releasing (GlaH or CsiD)
kauB 4-guanidinobutyraldehyde dehydrogenase RSPH17029_RS16005 RSPH17029_RS04350
lhgD L-2-hydroxyglutarate dehydrogenase or oxidase (LhgD or LhgO) RSPH17029_RS10685
ocd ornithine cyclodeaminase
oraE D-ornithine 4,5-aminomutase, beta (E) subunit
oraS D-ornithine 4,5-aminomutase, alpha (S) subunit
ord 2,4-diaminopentanoate dehydrogenase
orr ornithine racemase
ortA 2-amino-4-oxopentanoate thiolase, alpha subunit
ortB 2-amino-4-oxopentanoate thiolase, beta subunit
patA putrescine aminotransferase (PatA/SpuC) RSPH17029_RS20165 RSPH17029_RS16010
patD gamma-aminobutyraldehyde dehydrogenase RSPH17029_RS16005 RSPH17029_RS04350
prdA D-proline reductase, prdA component
prdB D-proline reductase, prdB component
prdC D-proline reductase, electron transfer component PrdC
prdF proline racemase RSPH17029_RS15945
PRO3 pyrroline-5-carboxylate reductase RSPH17029_RS15160
puo putrescine oxidase
put1 proline dehydrogenase RSPH17029_RS04265 RSPH17029_RS07235
putA L-glutamate 5-semialdeyde dehydrogenase RSPH17029_RS04265 RSPH17029_RS16005
rocA 1-pyrroline-5-carboxylate dehydrogenase RSPH17029_RS04265 RSPH17029_RS16005
rocD ornithine aminotransferase RSPH17029_RS04315 RSPH17029_RS20165
rocE L-arginine permease
speB agmatinase RSPH17029_RS07860 RSPH17029_RS17500

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory