GapMind for catabolism of small carbon sources

 

D-glucuronate catabolism in Cereibacter sphaeroides ATCC 17029

Best path

dctP, dctQ, dctM, uxaC, uxuB, uxuA, kdgK, eda

Rules

Overview: Glucuronate utilization in GapMind is based on MetaCyc pathways D-glucuronate degradation II (oxidation of 5-keto-4-deoxyglucarate, link), a related pathway via 5-keto-4-deoxyglucarate aldolase (link), or degradation via fructuronate (link). GapMind also includes a variation on the oxidative pathway with a glucarolactonase, as in Pseudomonas putida. MetaCyc pathway I (via L-gulonate and xylitol, link) is not reported in prokaryotes and is not described here.

18 steps (14 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
dctP D-glucuronate TRAP transporter, solute receptor component RSPH17029_RS10770 RSPH17029_RS17115
dctQ D-glucuronate TRAP transporter, small permease component
dctM D-glucuronate TRAP transporter, large permease component RSPH17029_RS10760 RSPH17029_RS17920
uxaC D-glucuronate isomerase RSPH17029_RS10775
uxuB D-mannonate dehydrogenase RSPH17029_RS10740 RSPH17029_RS17930
uxuA D-mannonate dehydratase RSPH17029_RS12260 RSPH17029_RS18320
kdgK 2-keto-3-deoxygluconate kinase RSPH17029_RS10785 RSPH17029_RS13575
eda 2-keto-3-deoxygluconate 6-phosphate aldolase RSPH17029_RS06600 RSPH17029_RS20365
Alternative steps:
dopDH 2,5-dioxopentanonate dehydrogenase RSPH17029_RS17095 RSPH17029_RS04350
exuT D-glucuronate:H+ symporter ExuT
garK glycerate 2-kinase RSPH17029_RS19190
garL 5-dehydro-4-deoxy-D-glucarate aldolase RSPH17029_RS18910 RSPH17029_RS12825
garR tartronate semialdehyde reductase RSPH17029_RS07320 RSPH17029_RS09040
gci D-glucaro-1,4-lactone cycloisomerase RSPH17029_RS20915 RSPH17029_RS18320
gudD D-glucarate dehydratase
kdgD 5-dehydro-4-deoxyglucarate dehydratase RSPH17029_RS17090 RSPH17029_RS12825
udh D-glucuronate dehydrogenase
uxuL D-glucaro-1,5-lactonase UxuL or UxuF RSPH17029_RS19585

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory