L-histidine catabolism in Rhizobium leguminosarum WSM1325

Best path

braC, braD, braE, braF, braG, hutH, hutU, hutI, hutF, hutG'

Rules

Overview: Histidine utilization in GapMind is based on MetaCyc pathways L-histidine degradation I (link) or II (link). These pathways are very similar. Other pathways in MetaCyc (III-VI) are not complete or are not reported in prokaryotes, so they are not included.

• all:
  • histidine-transport, hutH, hutU, hutI and hutG
  • or histidine-transport, hutH, hutU, hutI, hutH, hutF and hutG'
  • Comment: In pathway I, hutH deaminates histidine to urocanate, hutU hydrates it to 4-imidazolone-5-propanoate, hutI hydrolyzes it to N-formiminoglutamate, and hutG hydrolyzes it to formamide and glutamate. Formamide can be cleaved to formate and ammonia, but it could also be secreted, so this is not described. Similarly, formate may be oxidized to CO2 or secreted. Pathway II also involves conversion to N-formiminoglutamate, but then it is deaminated to N-formylglutamate by hutF, and hydrolyzed to formate and glutamate by hutG'.
• histidine-transport:
  • hisP, hisM, hisQ and hisJ
  • or PA5503, PA5504 and PA5505
  • or Ac3H11_2562, Ac3H11_2561, Ac3H11_2560, Ac3H11_2555 and Ac3H11_2554
  • or natA, natB, natC, natD and natE
  • or aapJ, aapQ, aapM and aapP
  • or braC, braD, braE, braF and braG
  • or hutV, hutW and hutX
  • or bgtA and bgtB
  • or BPHYT_RS24000, BPHYT_RS24005, BPHYT_RS24010 and BPHYT_RS24015
  • or permease
  • or Ga0059261_1577
  • or S15A3
  • or LAT2
  • or LHT
  • or SLC38A3
  • or PTR2
  • Comment: Transporters were identified using query: transporter:histidine:L-histidine:his

48 steps (36 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
braC	ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC	RLEG_RS16390	RLEG_RS15300
braD	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD)	RLEG_RS16415
braE	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE)	RLEG_RS16410
braF	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF)	RLEG_RS16405	RLEG_RS27560
braG	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG)	RLEG_RS16400	RLEG_RS30575
hutH	histidine ammonia-lyase	RLEG_RS28530	RLEG_RS28475
hutU	urocanase	RLEG_RS28540	RLEG_RS22985
hutI	imidazole-5-propionate hydrolase	RLEG_RS28525
hutF	N-formiminoglutamate deiminase	RLEG_RS28520
hutG'	N-formylglutamate amidohydrolase	RLEG_RS28535	RLEG_RS17895
Alternative steps:
aapJ	L-histidine ABC transporter, substrate-binding component AapJ	RLEG_RS08730
aapM	L-histidine ABC transporter, permease component 2 (AapM)	RLEG_RS08720	RLEG_RS34480
aapP	L-histidine ABC transporter, ATPase component AapP	RLEG_RS08715	RLEG_RS29285
aapQ	L-histidine ABC transporter, permease component 1 (AapQ)	RLEG_RS08725
Ac3H11_2554	ABC transporter for L-Histidine, permease component 2	RLEG_RS20610	RLEG_RS25950
Ac3H11_2555	L-histidine ABC transporter, substrate-binding component 2	RLEG_RS20605	RLEG_RS11315
Ac3H11_2560	L-histidine ABC transporter, ATPase component	RLEG_RS34565	RLEG_RS16245
Ac3H11_2561	L-histidine ABC transporter, permease component 1	RLEG_RS34650
Ac3H11_2562	L-histidine ABC transporter, substrate-binding component 1
bgtA	L-histidine ABC transporter, ATPase component BgtA	RLEG_RS26105	RLEG_RS25940
bgtB	L-histidine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
BPHYT_RS24000	L-histidine ABC transporter, substrate-binding component	RLEG_RS28495	RLEG_RS11315
BPHYT_RS24005	L-histidine ABC transporter, permease component 1	RLEG_RS28490	RLEG_RS34590
BPHYT_RS24010	L-histidine ABC transporter, permease component 2	RLEG_RS28485	RLEG_RS34585
BPHYT_RS24015	L-histidine ABC transporter, ATPase component	RLEG_RS28480	RLEG_RS34600
Ga0059261_1577	L-histidine transporter
hisJ	L-histidine ABC transporter, substrate-binding component HisJ	RLEG_RS11315	RLEG_RS34595
hisM	L-histidine ABC transporter, permease component 1 (HisM)	RLEG_RS11325	RLEG_RS34585
hisP	L-histidine ABC transporter, ATPase component HisP	RLEG_RS34600	RLEG_RS28480
hisQ	L-histidine ABC transporter, permease component 2 (HisQ)	RLEG_RS11320	RLEG_RS34590
hutG	N-formiminoglutamate formiminohydrolase
hutV	L-histidine ABC transporter, ATPase component HutV	RLEG_RS23930	RLEG_RS24025
hutW	L-histidine ABC transporter, permease component HutW	RLEG_RS23935	RLEG_RS15270
hutX	L-histidine ABC transporter, substrate-binding component HutX	RLEG_RS23940
LAT2	L-histidine transporter
LHT	L-histidine transporter
natA	L-histidine ABC transporter, ATPase component 1 (NatA)	RLEG_RS16405	RLEG_RS27560
natB	L-histidine ABC transporter, substrate-binding component NatB
natC	L-histidine ABC transporter, permease component 1 (NatC)
natD	L-histidine ABC transporter, permease component 2 (NatD)	RLEG_RS16360	RLEG_RS27550
natE	L-histidine ABC transporter, ATPase component 2 (NatE)	RLEG_RS16400	RLEG_RS30575
PA5503	L-histidine ABC transporter, ATPase component	RLEG_RS33120	RLEG_RS25940
PA5504	L-histidine ABC transporter, permease component	RLEG_RS33125	RLEG_RS00760
PA5505	L-histidine ABC transporter, substrate-binding component	RLEG_RS33115
permease	L-histidine permease
PTR2	L-histidine:H+ symporter
S15A3	L-histidine transporter
SLC38A3	L-histidine:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.