L-arabinose catabolism in Paraburkholderia sp. CCGE1002

GapMind for catabolism of small carbon sources

L-arabinose catabolism in Paraburkholderia sp. CCGE1002

Best path

araF, araG, araH, xacB, xacC, xacD, xacE, xacF

Rules

Overview: L-arabinose utilization in GapMind is based on MetaCyc pathways L-arabinose degradation I, via xylulose 5-phosphate (link); III, oxidation to 2-oxoglutarate (link); and IV, via glycolaldehyde (link). Pathway II via xylitol and xylulose is not represented in GapMind because it is not reported in prokaryotes (link).

glycolaldehyde-dehydrogenase:

aldA
or aldox-large, aldox-med and aldox-small

all:

arabinose-transport, araA, araB and araD
or arabinose-transport, xacB, xacC, xacD, xacE and xacF
or arabinose-transport, xacB, xacC, xacD, KDG-aldolase, glycolaldehyde-dehydrogenase, gyaR and glcB
Comment: In pathway I, isomerase araA forms L-ribulose, kinase araB forms ribulose 5-phosphate, and epimerase araD forms D-xylulose 5-phosphate, which is an intermediate in the pentose phosphate pathway. In pathway III, the 1-dehydrogenase xacB (which acts on the furanose form, not the usual pyranose form?) forms arabino-1,4-lactone, lactonase xacC forms arbinonate, two dehydratases form 2-dehydro-3-deoxy-L-arabinonate and 2,5-dioxopentanonate (α-ketoglutarate semialdehyde), and dehydrogenase xacF forms 2-oxoglutarate, which is an intermediate in the TCA cycle. (Fitness data suggests that L-arabinose 1-epimerase or mutarotase is also involved, perhaps in creating the correct epimer for the 1-dehydrogenase, but is not included in GapMind.) Pathway IV begins as in pathway III, to 2-dehydro-3-deoxy-L-arabinonate, followed by KDG aldolase to pyruvate and glycolaldehyde; the glycolaldehyde is oxidized to glycolate and then to glyoxylate, and combined with acetyl-CoA by malate synthase, which is a TCA cycle intermediate. (Other pathways for glyxoylate assimilation are known but are not represented here.)

arabinose-transport:

gguA, gguB and chvE
or araF, araG and araH
or araS, araT, araU and araV
or xacG, xacH, xacI, xacJ and xacK
or xylFsa, xylGsa and xylHsa
or araUsh, araVsh, araWsh and araZsh
or araE
or BT0355
or Echvi_1880
Comment: Transporters were identified using query: transporter:arabinose:L-arabinose:L-arabinofuranose:L-arabinopyranose:beta-L-arabinose:CPD-12045:CPD-12046

40 steps (29 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
araF	L-arabinose ABC transporter, substrate-binding component AraF	BC1002_RS13345	BC1002_RS15875
araG	L-arabinose ABC transporter, ATPase component AraG	BC1002_RS13340	BC1002_RS15880
araH	L-arabinose ABC transporter, permease component AraH	BC1002_RS13335	BC1002_RS15885
xacB	L-arabinose 1-dehydrogenase	BC1002_RS13330	BC1002_RS24230
xacC	L-arabinono-1,4-lactonase	BC1002_RS13325	BC1002_RS31150
xacD	L-arabinonate dehydratase	BC1002_RS17315	BC1002_RS29330
xacE	2-dehydro-3-deoxy-L-arabinonate dehydratase	BC1002_RS21800
xacF	alpha-ketoglutarate semialdehyde dehydrogenase	BC1002_RS08120	BC1002_RS34220
Alternative steps:
aldA	(glycol)aldehyde dehydrogenase	BC1002_RS20725	BC1002_RS22645
aldox-large	(glycol)aldehyde oxidoreductase, large subunit	BC1002_RS00450	BC1002_RS28420
aldox-med	(glycol)aldehyde oxidoreductase, medium subunit	BC1002_RS28415	BC1002_RS00445
aldox-small	(glycol)aldehyde oxidoreductase, small subunit	BC1002_RS00455	BC1002_RS28410
araA	L-arabinose isomerase
araB	ribulokinase
araD	L-ribulose-5-phosphate epimerase
araE	L-arabinose:H+ symporter
araS	L-arabinose ABC transporter, substrate-binding component AraS
araT	L-arabinose ABC transporter, permease component 1 (AraT)
araU	L-arabinose ABC transporter, permease component 2 (AraU)
araUsh	L-arabinose ABC transporter, substrate-binding component AraU(Sh)	BC1002_RS18255	BC1002_RS08325
araV	L-arabinose ABC transporter, ATPase component AraV	BC1002_RS30435	BC1002_RS21755
araVsh	L-arabinose ABC transporter, ATPase component AraV(Sh)	BC1002_RS18260	BC1002_RS23905
araWsh	L-arabinose ABC transporter, permease component 1 AraW(Sh)	BC1002_RS18265	BC1002_RS12465
araZsh	L-arabinose ABC transporter, permease component 2 AraZ(Sh)	BC1002_RS18270	BC1002_RS12465
BT0355	L-arabinose:Na+ symporter
chvE	L-arabinose ABC transporter, substrate-binding component ChvE	BC1002_RS26230
Echvi_1880	L-arabinose:Na+ symporter
gguA	L-arabinose ABC transporter, ATPase component GguA	BC1002_RS26235	BC1002_RS23905
gguB	L-arabinose ABC transporter, permease component GguB	BC1002_RS26240	BC1002_RS23900
glcB	malate synthase	BC1002_RS07585	BC1002_RS08970
gyaR	glyoxylate reductase	BC1002_RS10795	BC1002_RS08390
KDG-aldolase	2-dehydro-3-deoxy-L-arabinonate aldolase
xacG	L-arabinose ABC transporter, substrate-binding component XacG
xacH	L-arabinose ABC transporter, permease component 1 (XacH)	BC1002_RS03670	BC1002_RS31125
xacI	L-arabinose ABC transporter, permease component 2 (XacI)	BC1002_RS03675
xacJ	L-arabinose ABC transporter, ATPase component 1 (XacJ)	BC1002_RS23830	BC1002_RS28100
xacK	L-arabinose ABC transporter, ATPase component 2 (XacK)	BC1002_RS28100	BC1002_RS03680
xylFsa	L-arabinose ABC transporter, substrate-binding component XylF	BC1002_RS32550
xylGsa	L-arabinose ABC transporter, ATPase component XylG	BC1002_RS32540	BC1002_RS21780
xylHsa	L-arabinose ABC transporter, permease component XylH	BC1002_RS32545	BC1002_RS29645

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources